Question 1 1.1 Silica gel chromatography: This is known as the stationary phase in column chromatography. Firstly‚ the tapered exit of the column is sealed using porous material. This porous material serves as support for the packing material‚ and prevents it from exiting the pipe. Thereafter‚ silica gel is compacted into the glass pipe to make the separating column. In finishing preparation of the column‚ the solvent which is used as the mobile phase is then passed through the dry column. Then the
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Title: Principles and Practice of Agarose Gel Electrophorsis Objectives: To detect the size ‚ shape and charge of the each dye solution. Methods: Casting the Agarose Gel In this experiment .8% solution was used. By using a 250ml flask the buffer solution was prepared. Using the equation to make enough solution for the intire lab class the equation had to be multiplyed by five. The contents of this equation were added to the 250ml flask and swirled to evenly distrubute it contents
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Bernardine Date Due: 02/08/2013 PURISIMA‚ Dio Mark Angelo Date Submitted: 02/08/2013 Experiment No. 9 AGAROSE GEL ELECTROPHORESIS OF DNA Abstract _____________________________________________________________________________ Agarose is a polymeric cross-linked polysaccharide extracted from the seaweed agar. Agarose is used widely in gel electrophoresis because it gels at a lower temperature‚ does not contain the inhibitors of virus growth frequently present in agar‚ and has more uniform
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Gel Electrophoresis Adventure Intro The final goal of this lab was to successfully measure the size of different samples of DNA by placing each sample into a well in agarose gel and running a current through a charged chamber. The DNA samples will move through the gel towards the positive charge. Ideally‚ the DNA will move and create and sequence of smallest to largest. This lab exposes us to DNA technology. Backround Gel electrophoresis is used to separate macromolecules like DNA or RNA by size or
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the gel after electrophoresis. Gel 1 Gel 2 Lane 7. This is Maddie’s (MCB) sample. Gel 2 Lane 6. This is Madi’s (MN) sample. From our sample of the gel electrophoresis‚ both Madi and me are homozygous positive (+/+) for the Alu gene. This can be determined by looking at the ladder and comparing our sample to it‚ to find out if we are homozygous or heterozygous. Discussion For this lab‚ DNA from our cheek cells were separated through PCR‚ and singled out through gel electrophoresis
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Board of Directors of Canada Haircare Inc‚ our company manufactures and sells Hair Care products. Vstron waterproof hair gel is our new product‚ and it is a revolution and development of hair gel and its functions. Vstron waterproof hair gel provides a natural and strong hold on all kinds of hair styles‚ and most importantly‚ it is waterproofed. By using Vstron waterproof hair gel‚ consumers will no longer worry about their messy looks after they get wet. Our philosophy is: high-quality‚ natural and
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Gel electrophoresis is a routine laboratory procedure in biochemical studies that takes advantage of a protein’s amphoteric nature to determine its molecular weight and charge by running the sample through a gel matrix under the influence of an electrically charged field. A popular example of gel electrophoresis is Sodium dodecyl sulfate-polyacrylamide electrophoresis or SDS- PAGE which has been used in this experiment to supposedly determine albumin and casein’s molecular weights respectively. The
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BI338 Lab 1 Measuring the Success of Pisum sativum leaf cell fractionation: SDS-Polyacrylamide Gel Electrophoresis and Western Blot Analysis Introduction In order to evaluate the success of cellular fractionation‚ there are various methods that can be implemented that target specific proteins and/or receptors specific to particular organelles. SDS-PAGE (sodium dedocyl sulfate- polyachrylamide gel electrophoresis) is a technique used to separate proteins based on their mass. As different proteins
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Carbopol 940 could yield clear gel with desired rheological property. Carbopol 940 was selected as a gelling agent based on preliminary experiments and previous reports due to its widespread use in pharmaceutical formulations and fast dispersion in water (Sahoo et al.‚ 2014; Sharma and Bedi‚ 2014; Pillai et al.‚ 2015). Moreover‚ it is speculated that Carbopol with low concentration can be safe for nasal administration (Khan et al.‚ 2010). Carbopol is known to have mucoadhesive properties. It
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to the sol-gel transformation by proposing that the micellar unit of the gel state is identical with that in the sol. According to these authors: "All that is necessary is to assume that the particles become stuck together or oriented into loose aggregates‚ which may be chance granules or‚ more probably threads." This conception is based on a study of sodium oleate‚ for which they found that in spite of the enormous change in viscosity involved in the change from sol to gel‚ such properties
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