compound. Thus‚ instead of preparing one large dilution‚ if we take a concentrated sample of a particular compound and perform a series of dilutions with it‚ we can obtain various concentrations of the same compound. In the context of this experiment‚ for gel electrophoresis and absorbance spectrophotometry‚ we need to prepare a series of
Premium Chemistry Solution Concentration
Page 1 Analysis of transcriptional regulation of the arabinose operon promoter using expression from a green fluorescent protein encoding reporter gene + + KIRA FERNANDEZ ‚ CLAIRE MARKEY * ‚ JESSE PIERATTI + Department of Biological Sciences ‚ and Department of Nutrition and Dietetics*‚ Messiah College‚ Mechanicsburg‚ PA 17055 Page 2 ABSTRACT This study investigates gene regulation and how environmental arabinose and/or glucose can interact with geno
Premium DNA Gene Bacteria
3.2 DNA Extraction The DNA will be extracted from the Nemipterus samples according to Wizard® Genomic DNA Purification Kit (Promega) protocols. The first step of is cells and nuclei will be lysed by adding 120 µl of 0.5 Molar ethylenediaminetetraacetic acid (EDTA) to 500 µL of Nuclei Lysis Solution in a eppendorf tube‚ then it will be chilled on ice until the solution turn cloudy. The second step is 0.5 cm of Nemipterus sample tissue will be minced to fine powder. The fine powder of fresh Nemipterus
Premium DNA Protein Molecular biology
molecule and formed hydrogen bonds with the silica gel (structure of O=S=O). In order to acquire the fluorenone crystals‚ ethyl acetate was introduced second because it is a polar solvent. Hydrogen bonds were formed between the ethyl acetate and the fluorenone molecules‚ so the fluorenone molecules were displaced from the silica gel adsorbent. Because ethyl acetate is held to the silica gel by hydrogen bonds between the oxygen atoms of the silica gel and the hydrogen atoms of the ethyl acetate‚ the
Premium Oxygen Chemistry Distillation
A 0.8% agarose gel was used as the medium for the DNA solutions because it has a better separation of large DNA fragments due to the larger pores throughout the gel. The electrodes on each end of the apparatus allowed the fragments to migrate across the gel. The opposite end of the DNA-containing wells has a positively charged electrode that attracts the DNA‚ which is negatively charged
Premium DNA Escherichia coli Bacteria
Extraction and Analysis of Plasmid DNA from E. coli Cells Introduction A plasmid is an extra-chromosomal element‚ often a circular DNA. Since a plasmid is by definition an extra-chromosomal element‚ it cannot make use of any origin of DNA replication in a chromosome (BP site). Meaning that DNA synthesis within a plasmid depends on having an origin of DNA synthesis of its own. Plasmids are often found in bacterial cells‚ in which they are used as transfer agents for transmitting various antibiotic
Premium Molecular biology DNA Plasmid
students in a BIOL 3600 laboratory. Buccal cells were extracted from the BIOL 3600 students‚ later purified‚ and then cut with two restriction endonucleases‚ MseI and RsaI. The now cut DNA was mixed with a blue loading dye and run on a 1.0% agarose gel (with added TAE buffer and Sybr Safe) at 110 volts. The finished product had bands of diverse base pair lengths‚ differing from student to student. During the RFLP analysis
Premium Rape Assault Criminal law
agarose was poured into the prepared gel-casting tray for 30 minutes until it became solid. The well-forming comb was then removed and 1X TBE buffer was added to fill in wells‚ to create a smooth buffer surface. Using a micropipette 25 µL of my dye sample mix was added to the well. The gel was run at 130 V for 30 minutes. The gel was stained for 10 to 15 minutes using ethidium bromide. The gel was viewed using UV transillumination and a photograph was taken of the gel. Analysis of Genotype and Allele
Premium DNA Gene Genetics
series of labs‚ we were able to extract and isolate our mitochondrial DNA‚ nuclear DNA‚ and cellular DNA to then amplify a part of an intron in the tissue plasminogen activator gene (TPA) through Polymerase Chain Reaction (PCR) and run on an agarose gel to detect the presence or absence of a transposable element known as an Alu element. The main purpose of this experiment was to use our class as a population‚ to test whether or not we were in Hardy-Weinberg Equilibrium. The Hardy Weinberg Law states
Premium Genetics Gene Evolution
“compositase” to find the identity of the plant in the pot. This process uses electric currents to draw DNA out from the wells formed in the gel because the DNA is negatively charged and it would try to move through the gel to get away from the negative side of the electric current. This allows the scientists to see it’s behavior as it moves through the gel as the smaller proteins move more quickly than the larger ones. Since DNA is colorless the scientists stain the sample in order to visualize
Premium Sexual intercourse Physician Academy Award for Best Actor