Abstract:Conjugation is a natural occurring process that involves the transfer of DNA from one cell into another through a physical connection between the cells. In the following experiment‚ two strains of Escherichia coli bacterial cells (donor F’lac+strs and recipient F-lac-strr) underwent conjugation to produce a transconjugant strain (F’lac+strr). MAC plates and streptomycin were utilized to determine if conjugation had occurred. When plated‚ the donor colonies appeared red and the recipient
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3. Study your gel electrophoresis results: a) Which solution sample contained a single dye: S1‚ S2‚ or S3? How do you know? S3 contained a single dye because it only had one band. b) The molecular weights for the dyes are 452.38 atomic units (au) for orange G‚ 669.98 au for bromophenol blue‚ and 538.62 au for xylene cyanole. How do these weights compare with your original conclusions about the weights of the dyes? Base on the order of movement of the dyes through the gel (yellow moved the
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and Wang 2011). The first objective of this experiment was to use two restriction endonucleases‚ EcoR1 and Xba1‚ to cut pUC19 and pBK-CMV. To ensure that the plasmids were successfully cut‚ analysis of the plasmid was carried out using gel electrophoresis. Gel
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Expressing and Purifying the Recombinant form of Green Fluorescent Protein (rGFP) from the E.coli strain using Ni2+ agarose affinity chromatography technology Abstract The purpose of this experiment was to express and purify the his6-tagged recombinant form of GFP (rGFP) from the organism E.coli using Ni2+ agarose affinity chromatography. The expression of rGFP was confirmed qualitatively using the UV light and was expressed in the E.coli strain BL21 (DE3) (-- removed HTML --) (-- removed HTML
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glass tube and an agar-water gel. In glass tube test‚ two cotton plugs submerse with two different substances – hydrochloric acid (HCL) and ammonium hydroxide (NH4OH) on the other end of the tube. By their molecular weight‚ NH4OH has a small molecular weight that diffuses at a faster rate. While the formation of a white cloud was first occurred at the end of the tube containing HCL with a large molecular weight. In agar-water gel test‚ using agar plate and agar-water gel‚ 2 different substrate were
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1. Identification of Some Macromolecules 1) What are 2 types of treatment controls‚ and explain each. Positive control: gives positive result if experimental conditions were followed correctly Negative control: gives negative result if experimental conditions were followed correctly 2) What are the most abundant elements in living material? Carbon‚ Hydrogen‚ Oxygen‚ Nitrogen‚ Sulfur and Phosphorus 3) What are the 4 major biological macromolecules? Carbohydrates (monosaccharides &
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Using Kanamycin Resistance Bacteria to find the Sources of Contamination of Three Chicken Farms Introduction: Kanamycin is a common antibacterial that interferes with bacterial growth‚ by inhibiting protein synthesis‚ and causing the mistranslation of mRNA. Kanamycin is commonly used in chicken feed to keep harmful bacteria from getting into the eggs and producing healthier chickens. Recently reports of severe gastroenteritis have been linked to eating raw or undercooked eggs. This has led to
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departments are amethocaine gel (or An Gel cream) and EMLA cream. Amethocaine gel is used exclusively in the general emergency department I work in for intravenous cannulation or venepuncture in children. Amethocaine gel is considered to be the more favourable choice in topical anaesthetics and replaced EMLA cream. In fact‚ the staff was given little choice but to use the Amethocaine gel as EMLA was completely removed from the department. As little education was given on Amethocaine gel when it was introduced
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Electrophoresis is commonly used to separate proteins using an applied electrical field. SDS-PAGE separates proteins by molecular weight‚ using sodium dodecyl sulfate (SDS; also known as lauryl sulfate) to denature them and a discontinuous polyacrylamide gel as a sieving matrix. The net charges of folded proteins are not dependent upon molecular weight. Rather‚ charge is determined by the sum of the individual amino acids’ R-group charges together with the molecular radius of the tertiary structure. Therefore
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biotechnology research company that is developing two different technologies in order to produce a solution to this problem. One is the PolySel© - an absorbent gel formula which can be used together with various kind of active substances. The other is an antimicrobial compound containing nanosilver. These two will combine to Ag+el©- an easy-to-use gel with significantly higher antimicrobial effect than competing products. The market is today dominated by a few large players who market a wide range
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