The technique of gene cloning has important applications in medicine‚ biotechnology‚ agriculture and research. Discuss two applications of this technique‚ which in your opinion‚ have had significant impact on society. Gene cloning is achieved by inserting a required gene into a vector DNA. This produces Recombinant DNA and when introduced to a host cell the vector is copied. It divides to produce lots of copies of the gene of interest (Willey‚ Sherwood and Woolverton‚ 2012). There are many applications
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The word gene comes from the word for origin’ in Greek. Genes are sections of DNA that determine the structure of a protein. They are the foundation of all life on earth. They form and decide the characteristics of every living creature and the genes that exist today are there because they have been successfully reproduced in the past. The DNA that determines the person that we grow into is created from half of each of our parents DNA. In the process of evolution certain genes have been eradicated
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article‚ “Elephant Genes Hold Cancer-Fighting Secret‚” the author‚ Laura Geggel‚ explains how researchers have recently discovered that cancer is less common in elephants than in humans because they have more copies of a gene that stops tumor growth. Researchers that understand how this gene has evolved and why it works to stop cancer growth in elephants may be able help find ways to treat or cure cancer in humans. Researchers conducted a study that focused mainly on the TP53 gene‚ which normally codes
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Longevity pathways converge on autophagy genes to regulate life span in Caenorhabditis elegans Autophagy‚ translated from Greek means to ‘self eat’. It is a basic catabolic mechanism involving cell degradation of unwanted or dysfunctional cellular components through the lysosome. Autophagy has been speculated to alter lifespan in organisms. Autophagy is triggered in response to varied stress and physiological conditions like food deficit‚ hyperthermia‚ etc. At molecular level‚ an autophagy pathway
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Examining the RNA Interference Mechanism in the dpy-13 Gene in C. Elegans Through Feeding Mehdi Misto Lab: Monday 1:00 – 4:50 PM 11 December 2012 Introduction: RNA interference‚ or RNAi‚ is a biological process in which RNA molecules reduce the gene expression of an organism. This is done typically by causing the destruction of specific mRNA molecules. RNAs are direct products of genes‚ these small RNAs can bind to other mRNA molecules to either increase or decrease their activity like in the example
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Application of Molecular Techniques for Detection of Disease Resistant Genes in Tomato Breeding Lines for Guatemala Objectives: 1. Evaluate and modify methods for detection of Fusarium Race 2 resistance gene. 2. Evaluation of two step protocol for detection of Mi-1 gene. 3. Verification of marker for Ty-1 and evaluation for marker in Guatemala breeding lines. (This is a Geminivirus that is transmitted by the whitefly) 4. Use of molecular markers to detect geminivirus resistance source for Gu143
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Using P-element Induced Male Recombination to Generate a Deletion in the DMAP1 Gene on Chromosome Two in Drosophila melanogaster Abstract: The goal of this study was to induce a deletion in the DMAP1 gene on chromosome two in Drosophila melanogaster through P-element mobilization. The DMAP1 gene may be an essential gene‚ however not much is known about it. We attempted to uncover the function of DMAP1 by creating a series of genetic crosses and selecting for brown-eyed non-stubble male flies
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Segregation. This experiment investigated the effects of complete and incomplete dominance on the behavior of two different genes for chlorophyll production in tobacco. Two sets of approximately 50-100 tobacco seeds were planted for one week. The plants were the F2 result of two sets of monohybrid F1 crosses Gg x Gg and CyCg x CyCg. A chromosome for these plants has two sets of genes. The gene at one locus has two alleles that code for normal chlorophyll production. Dominant allele represented as G produce
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"Gattaca"‚ written and directed by Andrew Niccol‚ depicts a futuristic world of a cold society‚ where one’s resume exists in their cells and genes. Although genes play a significant part to a successful life in this world‚ the path and success of one’s life is not determined solely on DNA as demonstrated by Eugene‚ Anton and in particular‚ Vincent who clearly "exceeds his potential". Vincent Freeman is cast into society’s redundant section as being an "invalid"‚ however he overcomes this burden
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1. Introduction Since the discovery of plasmid‚ various methods have been developed to isolate plasmid DNA. All the methods have one common and important target of isolating plasmid DNA of high quality and quantity in less time. These methods are not completely safe because of use of toxic chemicals compounds. The developed protocol for plasmid extraction is based on the alkaline lysis method of plasmid preparation (extraction at pH 8.0) with slight modifications. Cell lysis reagent sodium dodecyl
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