Title: pH and buffer solutions Aim This experiment was carried out to determine the role of buffer solution and the factor which affect the buffer capacity. Besides‚ this experiment was carried out to investigate the solubility of protein casein over a range of pH concentration. This experiment also was carried out to determine the isoelectric point of the casein and the effect of the isoelectric point toward the casein solution. Methods Verification of the Henderson-Hasselbalch equation
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The starting material for this lab was the dialyzed sample (stored at -20ᵒ C) from the previous lab. The CM sephadex resin (taken in a 50 mL tube) was already made swollen using Buffer C (20 mM HEPES‚ pH 7.9; 1 mM EDTA; 50 mM KCl). The dialyzed sample was thawed to the room temperature and gently poured over the resin. The tube was capped and kept on a rocker at room temperature for 1 hour. The tube was then centrifuged in a HS-4 rotor at 2500 rpm (1200g) for 5 minutes at 4ᵒ C. Supernatant was discarded
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EXPERIMENT NO. 1 INTRODUCTION TO LAB INSTRUMENTS. 1. BREADBOARD We should be familiar to the following things about a breadboard: * What is a breadboard and what is it used for? * How does it work? * Setting Up. * Limitations. What is a breadboard and what is it used for? A breadboard (or protoboard) is usually a construction base for prototyping of electronics. The term "breadboard" is commonly used to refer to a solderless breadboard (plugboard). It was designed by
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concentrations of solutes in and out of the cell. Filtration is what is used to remove solid particles and they can be removed by passing through a liquid and gas. The information above is very important because it is exactly what everything in this lab will be about. It explains in detail what every single little definition and important
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integral part of every job and not a separate entity. In this document‚ only health and safety aspects will be considered. The terms "job" and "task" are commonly used interchangeably to mean a specific work assignment‚ such as "operating a grinder‚" "using a pressurized water extinguisher‚" or "changing a flat tire." JHAs are not suitable for jobs defined too broadly‚ for example‚ "overhauling an engine"; or too narrowly‚ for example‚ "positioning car jack." What are the benefits of doing a Job Hazard
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put half a spoon of zinc fillings. We add 20 ml of 3M NaOH solutions in it. We placed the beaker on the hot plate‚ which we had turned on before. We put the penny into the beaker. After it started to boil‚ we removed the penny from the beaker‚ using tongs. DATA COLLECTION AND PROCESSING: Observations: The color changing process took longer than we had expected. We observed that after putting the penny in the beaker‚ the zinc powder surround the penny‚ which helped it changed its color. We
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Molar Mass Lab 3.09 Molar Mass of Compounds Purpose: The purpose of this experiment is to determine the formula of a copper sulfate hydrate by heating it up. Materials: Hydrate‚ crucible‚ balance‚ Bunsen burner and scale Procedure: First you measure the mass of the empty crucible and record that mass. Then you should fill the crucible with 3-5 scoops of the hydrate and record that mass as well. Heat the hydrate filled crucible above the Bunsen burner for 10 minutes for all f the water to evaporate
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The identification of acids and bases using a natural indicator Purpose: The purpose of this lab practice is to identify the pH of certain solutions and if they are acids or bases. Introduction: In chemistry‚ pH is a measure of the activity of the (solvated) hydrogen ion. p[H]‚ which measures the hydrogen ion concentration‚ is closely related to‚ and is often written as‚ pH. Depending on the pH solutions will be acids or bases. An acid is a chemical compound that dissociates in solution‚ releasing
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done. Once equilibrium is achieved‚ or when the beam is not moving at a horizontal position‚ we can calculate for the unknown forces applied through the utilization of this principle. We balance the system given the ample forces acting on it. In general‚ application of Newton’s Second Condition of Equilibrium is applied here. By applying it‚ we could get the magnitude of one force acting on it‚ considering the other forces of known magnitudes. Thus‚ if weight is one of the forces‚ we could really
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is involved with the cannon being fired then the force of gravity would cause the cannon to start to slow down‚ wouldn’t go as far and the direction or trajectory of the cannon ball would be a curved line going down. Purpose: The purpose of this lab is to give the student a better understanding about projectile motion and to understand Newtons theories and laws of motion. We will be firing the plastic ball from the projectile launcher three times from eight different angles from the ground &
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