Alyssa Caparelli Organic Chemistry 12A Professor Alston October 28‚ 2014 Isolation of Chlorophyll and Carotenoid Pigments from Spinach Purpose The purpose of this experiment was to isolate ß-carotene‚ chlorophyll-A‚ and chlorophyll-B from spinach using column chromatography. Spinach was dehydrated using ethanol‚ and the pigments were extracted with dichloromethane. The spinach extracts were dried using CaCl2. Then‚ the solid pigments were run through a column using a non-polar solvent‚ hexane
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Katerina Baeza Hernandez | | | | | | | |Zn |Mg |Cu |Pb | | |(+) clear liquid but the |(+) a black spot appeared on|(-) no reaction occurred |(-) no reaction | |Pb(NO3)2
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Wanni Lin Biology 110 March 2‚ 2015 DNA Lab BACKGROUND In this laboratory experiment‚ students were introduced to DNA electrophoresis. DNA electrophoresis is an instrument that many forensic scientists use to get a DNA fingerprint as an evidence for crimes. Not only can it be used for forensic science‚ people can use this for paternity test‚ as well as look for evolutionary relationships among organisms. Agarose is used to make the gel that the DNA fragments are going into. Since DNA particles are
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graduated cylinder‚ 50 mL graduated cylinder‚ small and large test tube‚ 50 mL and 150 mL beakers‚ 50 mL Erlenmeyer flask‚ digital top-loading balance‚ unknown liquids for density determination‚ irregular solids for density determination‚ safety glasses‚ Lab manual that was used for measurement. Introduction The International System of Measurement (SI) is used worldwide and has been adopted as the official system of measurement by most countries. It is commonly called the metric system. Our traditional
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Effects of Temperature‚ pH‚ Boiling‚ and Hydroxylamine on the Enzyme Peroxidase Extracted From Brassica rapa Abstract In this experiment the enzyme peroxidase was extracted from from a turnip‚ Brassica rapa‚ and tested under different conditions. The effects of temperature‚ boiling‚ pH‚ and a competitive inhibitor were tested. The enzyme was tested at temperatures of 4°C‚ 24°C‚ 32°C‚ and 48°C. As the temperature increased‚ so did the activity of the enzyme
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1 x 400mL±5% glass beaker 2 x 500mL±5% glass beaker 1 roll of tape Ball-point pen Stirring Rod Procedure 1. Put on safety goggles. 2. Fill 2‚ 500mL beakers fully with the 1L glycerol. 3. Put a piece of tape on each 100mL graduated cylinder‚ and using the pen‚ label ‘10°C‚20°C‚30°C‚40°C and 50°C’‚ respectively Procedure for 20° condition 1. Pour 50mL of glycerin from one of the 500mL beakers into the graduated cylinder labeled ‘20°C’ 2. Take the thermometer and place it in the ‘20°C’ graduated cylinder
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terms of their sterility and potential uses. Both solutions must be sterile because both could be put into a human body. Normal Saline is used in an IV so it enters the blood‚ you wouldn’t want the Nasal Irrigation in your IV so making sure you are using the correct solution is vital. With the Nasal Irrigation solution it can be used in a Nedi Pot so if the solution mixture is not sterile bacteria could enter the brain. 5. Why should saline for irrigation never be used for intravenous uses? If
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Title: Observation of Macroscopic Chemical Changes‚ Alice Kimbrell‚ Chemistry 101-DS01:74589‚ 5/31/2013. Purpose: In this lab‚ I will observe macroscopic changes that occur when mixing together certain chemicals and household cleaners. The mixing of the chemicals provided with each other and with acid/base indicators demonstrates how matter can change‚ and how chemistry can be seen with the naked eye. By mixing household cleaners with an acid/base indicator I hope to demonstrate how these
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Tori Suchy Physical Science Honors 1st block October 4‚ 2012 Balloon Powered Car Problem: In order to create a race car that can travel a minimum of eight meters powered by a nine inch balloon. We need incorporate Newton ’s Law of Motion including speed‚ velocity‚ and acceleration. Hypothesis: If a car were to be made out of homemade materials‚ then it would be possible for it to travel 8 meters powered by a nine inch balloon. Independent Variable: building the car from homemade materials
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Meal Worm Cellular Respiration Experiment Introduction Cellular respiration provides energy to all living organisms by collecting energy from food molecules. The cell then rearranges complex molecules to simpler molecules and uses that energy to make a molecule that stores energy called adenosine triphosphate also known as ATP. Cellular respiration can occur with or without oxygen. When it occurs without oxygen‚ it is called anaerobic respiration. There are very few organisms that can get
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