Introduction Transformation is a genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material from its surrounding through the cell membrane. The arabinose operon changes AraC from a repressor to an activator; in this experiment the pGLO plasmid has been designed with a modified operon so that in the presence of the arabinose the bacterial cells which have been transformed by the pGLO plasmid will fluoresce due to the production of GFP. SDS-PAGE
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NA transformation of E. coli: The two plasmids were added to individual tubes containing E. coli and one with no plasmids. The three samples of E. coli were heated in a 42°C water bath for 90 seconds to heat shock the bacteria so that the plasmids would be taken up by the E. coli. These samples were then incubated at 30°C for half an hour and then plated on LB agar. Each tube was plated on an LB plate and a LB + ampicillin plate. Ampicillin is an antibiotic that is effective against E. coli‚ both
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Genetic transformation happens when an organism is altered by the introduction of new genetic information which is merged into the organism’s genome. Bacterial transformation is a type of genetic transformation that was used in lab and mainly used due to the single celled nature of bacteria. In this lab‚ the engineered pGLO plasmid is integrated into E. Coli bacteria‚ and adds the genes which code for the proteins GFP in the modified bacteria’s genome (Hanahan‚ Studies on transformation of Escherichia
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Introduction Genetics is the study of units of heredity. Genes determine almost everything about what makes humans humans and genes are passed from parent to offspring‚ because genes have a strong influence on all organisms. Genetics a very broad scientific discipline and the father of genetics is Gregor Mendel. Gregor Mendel studies how genes were inherited in pea plants by crossbreeding thousands of those and discovering their patterns and characteristics. In 20th century the technology has advanced
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Activity 33 PS-2826 Latent Heat of Fusion Thermodynamics: phase change‚ latent heat of fusion‚ melting Qty 1 1 1 1 1L 0.5 L 1 Equipment and Materials PASPORT Xplorer GLX Fast-Response Temperature Probe (included with GLX) Basic Calorimetry Set (1 calorimeter cup) Balance Water‚ about 15 degrees warmer than room temperature Ice‚ crushed Towel Part Number PS-2002 PS-2135 TD-8557 SE-8723 Purpose The purpose of this activity is to determine the amount of thermal energy needed to change a specific
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The purpose of this experiment was understand the process of transformation and the effects on gene expression. The pGLO(-) culture had growth on the LB medium‚ while the LB amp and LB amp + ara mediums had no growth. It was expected that the LB medium had growth on the plate because it served as a control. The LB amp and LB amp + ara had no growth or glow under UV light because they were not successfully transformed and still contained the antibiotic ampicillin that prevented the growth of E. coli
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Specific Heat Lab Report “Here are the facts we confront; No one is against conservation. No one is against alternative fuel sources.” – J. D. Hayworth Research Question: Does the number of hydrocarbons that a fuel is composed of affect how much it will ignite? If so‚ consider the following: What is the quantity and concentration of the combustion that is released and how can that be measured in other (more coherent) means? These are the fuels that were seasoned throughout the
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Transformation in bacteria is the genotype alteration by the uptake of naked‚ foreign DNA from the environment. This concept of transformation was first discovered when Fred Griffith an experiment using mice and strains of pneumonia. Griffith concluded that a “principle” was transferred from heat-killed S strains to the R strains‚ which transformed them into deadly S strains. Oswald Avery later determined‚ through a series of experiments‚ that DNA was the “principle” that caused the R stains to become
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Reshmi Radhakrishnan‚ TA: Jared Tues Rm 24 Experiment 6 Purpose: To analyze if diploid yeast strain heterozygous for ARG8 ADE2 HIS3/arg8 ade2 his3 on chromosome XV and TRP1/trp1 on chromosome IV irradiated with UV light underwent mitotic recombination to form homozygous recessive ade2/ade2 mutants. The ade2/ade2 mutant are selected then plated on different media to observe if the homozygous recessive mutants were due to mitotic recombination. Controls: If all the red cells grew on the CSM-Trp
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Lab Report: Mendelian Genetics Introduction: In 1866 an Austrian monk‚ Gregor Mendel‚ presented the results of painstaking experiments on the inheritance patterns of garden peas. Those results were heard‚ but probably not understood‚ by Mendel’s audience. Now‚ more than a century later‚ Mendel’s work seems elementary to modern–day geneticists‚ but its importance cannot be overstated. The principles generated by Mendel’s pioneering experimentation are the foundation for genetic counseling so important
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