"Genetic transformation lab report using heat shock" Essays and Research Papers

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    main purpose of the experiment is to investigate heat transfer processes using the tubular heat exchanger which is a specific type of heat exchangers. Moreover‚ the primary goals are to compare the two types of flow cases‚ co-current flow and countercurrent flow as well as taking the analyses of their temperature changes through the graphical curves and observing the heat transmission via the operating apparatus. The working principles of the tubular heat exchanger is analyzed and the fluid temperate

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    Module : Heat Transfer – Free Convection and Radiation Laboratory Date : 22nd March 2012 CONTENTS INTRODUCTION 3 AIMS & OBJECTIVES 3 Objectives 3 To investigate Free Convection and Radiation 3 Theory 3 EXPERIMENT 3 Apparatus Used 3 Procedure 4 RESULTS‚ CALCULATIONS‚ OBSERVATIONS & CONCLUSIONS 5 Observations During Tests 5 Table 1 5 Table 2 5 Calculations 6 Calculating Power (Watts) 6 Calculating Heat Transfer Emissivity (Ɛ) 6 Emisssivity of a black body

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    Lab Report (Scientific Paper) 2: Bacterial Transformation;DNA Extraction Part I & II:Total Genomic Extraction & Plasmid Extraction;Electrophoresis By:Chris Foster Abstract: We conducted three experiments that included a Bacterial Transformation‚ a two process DNA extraction‚ and a final procedure using gel electrophoresis. The Bacterial Transformation lab was performed to prepare the plasmid into a bacteria and to use that bacteria to amplify the plasmid in order to make large quantities

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    Calorimetry and Specific Heat Tessa Williams Chemistry 111 11/13/13 Abstract: In this experiment‚ the specific heat and the density of an unknown metal was determined in order to identify the unknown metal. The average specific heat of the unknown metal was 0.197˚C and was determined using a calorimeter. The density of the unknown metal was 6.57 g/mL and was determined using a cylinder and displacement. Using the specific heat value of the unknown metal and its density‚ it

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    Dna Transformation Lab

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    Conclusions of DH5α DNA transformation with red colonies resistance to ampicillin and the lacZ gene Introduction: In this experiment‚ a plasmid with a gene that has resistance to the antibiotic ampicillin and has lacZ is used to transfer the resistance into E. coli bacteria in red colonies. This same technique is used to give diabetics their insulin‚ and to give dwarfs growth hormones. The point of this lab is to give the groups an idea how DNA can

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    E. Coli Transformation with a Plasmid DNA Containing the GFP Gene Introduction: Bacterial transformation is the process of bacteria taking in and expressing exogenous DNA. This has led to many other discoveries. In order for bacterial transformation to occur the bacteria must be in a certain physical state to be able to take in DNA. This is called competency and it allows the cell membrane to be permeable so DNA can pass through. Currently researchers are studying the transformation of E. Coli

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    In part A of the experiment the two values obtained for the specific heat capacity of the metal were about .0500 J/mol. This is a large deviation‚ with both of these values identifying very different metals. The average heat capacity identified the metal used as brass. The average heat capacity calculated was .3776 J/mol and the actual heat capacity of brass is .375 J/mol. The second run displayed the closest value to the actual value. A source of error that may have caused a large divide in the

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    Heat of Neutralization Lab   Objective:​  The objective of this lab was to measure‚ using a calorimeter‚ the energy changes  accompanying neutralization reactions.    Background:​  Chemical changes are always accompanied by a change in energy‚ typically as  heat. If the reaction releases heat  (ΔH  <  0) then the reaction is exothermic. If the reaction  absorbs heat  (ΔH  >  0) then the reaction is endothermic. The quantity of heat is measured  experimentally by having the reaction take place in an insulated container called a 

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    Abstract: The topic of this research involved the occurrence of genetic transformation in bacteria (E. Coli). More specifically‚ a previously prepared pGLO plasmid--which consisted of the gene to be cloned--was used to transform non-pathogenic bacteria. The pGLO plasmid contained a gene for the Green Fluorescent Protein (GFP) from a bioluminescent jellyfish and a gene for resistance to ampicillin‚ an antibiotic. Essentially‚ we wanted to determine the conditions of the bacteria that would glow.

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    Specific Heat Lab

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    Ten Minutes of Specific Heat Remi Adams East Rowan High School AP Environmental Science Abstract Specific heat and climate were the primary focus of this lab. Specific heat is defined as “the measure of the ability of a substance to change temperature”. The purpose for carrying out this lab was to determine the specific heat/rates of both soil and water‚ and then comparing them. Students also were to relate specific heat to climate. Students were to determine which substance expressed

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