there are distinct differences between the cell walls of gram-positive and gram-negative bacteria. In the case of gram-positive bacteria‚ the cell wall is comprised of 60-90% peptidoglycan and is very thick. There are numerous layers of teichoic acid bound with peptidoglycan thereby creating very thick cell membranes which causes the cell wall to take up large quantities of basic dye and appears purple. (Hands-on-Labs. (2012)). Conversely‚ gram-negative bacteria cell walls are much thinner with an
Premium Bacteria Gram staining Staining
microorganisms is a practice that aids in Increasing ones knowledge of a laboratory setting and it helps improve on Using sterile technique. Isolates of soil microbes can be categorized and Characterized based on a number of criteria ranging from gram-staining Which is done for this project to enumeration which is quantitative description Based on the amount of microbial colonies available. The Antimicrobial Activity of three different microbes were also tested and results were obtained
Premium Bacteria Microorganism Microbiology
By Felicia Jin ABC^2‚ Make-A-Wish and Chinese clubs sold Valentine grams and treats Feb. 9-13‚ bringing the “sweets to sweeties” and funding for good causes. The gifts were purchased during the week and distributed to classes today. ABC^2 (Academically Better Children’s Club) held a bake sale for the first time in the Main Hall and will be donating their proceeds to the St. Jude’s Hospital. “It’s hard to say how much money we’re trying to raise because it’s our first time‚ but we’re hoping for a
Premium Orchestra High school
Since it was gram positive‚ I knew my organism had a thick peptidoglycan and no outer membrane. After finding out my organism B was gram positive‚ I did the catalase test on it. After adding the two drops of H202 and seeing bubbles‚ I ruled out Streptococcus lactis. My organism did have the catalase enzyme; meaning it was
Premium Bacteria Staining Gram staining
The Urea test was used to test both Unknown Gram-positive and Unknown Gram-negative bacteria. One colony of bacteria was used from the Unknown 13A plate and Unknown 13B plate in order to inoculate the Urea Slant. After the slant was inoculated‚ the cultures were placed in an incubator at 35ºC for Gram-positive bacteria‚ and 37ºC for Gram-negative bacteria for a total of 48 hours. If the Urea test were positive‚ the color of the slant would have changed to a hot pink color‚ which indicated the bacteria’s
Premium Bacteria Microbiology Metabolism
is to distinguish and indentify an unknown bacterium. There are several tests that can help one eliminate and narrow down the options. The most useful test‚ and the very first one done‚ is a gram stain. This test will tell whether the bacterium is gram-positive or gram-negative. After the type of gram stain is identified‚ the tester has a wide array of differentiating tests at their disposal. Based on the results from these tests‚ and the numerous others that are available‚ one can accurately
Premium Bacteria Microbiology Meningitis
smear my bacteria on a liquid medium. I then proceeded to incubate the medium for 24-48 hours. 1. GRAM STAIN The next step I took in finding my unknown bacteria was to gram stain it. This is used to differentiate the bacteria. The different staining reagents are: crystal violet‚ grams iodine‚ acetone-alcohol‚ and grams safranin. Under the microscope it was a pink color‚ which means Gram Negative. Also‚ the shape was a rod. 2. KLIGER’S IRON
Premium Bacteria Microbiology Gram staining
Question 1 of 12 2.0 Points All of the following are considered microbes except A.viruses B.bacteria C.protozoa Correct D.worms Answer Key: D Question 2 of 12 2.0 Points Microbiologists investigate problems by designing and carrying out experiments. What is true about the scientific method? A.a hypothesis is the definitive explanation to account for the observation and therefore does not need to be tested B.a prediction is the factor that can change
Free Bacteria Staining Gram staining
taps. Women had to travel 3 km to the Suru River to get water. The Gram Sabha got together to discuss the problem. Many people came up with short-term and long-term suggestions for dealing with the problem at hand‚ e.g.‚ piping water from the Suru and making an overhead tank‚ deepening the hand pumps and cleaning the wells‚ conserving and recharging water through watershed development. After the discussion in the Gram Sabha‚ the Gram Panchayat discussed the suggestions and decided that the money it
Premium Water supply Local government in India Panchayati raj
the plate‚ it could be seen that the unknown had growth and formation of colonies. The Gram stain was chosen next to determine if the unknown was gram-negative or gram-positive. After looking at the gram stain under the microscope with the oil immersion it could be determined that the unknown was gram-negative due to the fact that the bacteria was pink in color and rod shaped. After determining the unknown was gram-negative‚ other bacteria’s were able to be eliminated from the options. The Triple Sugar
Premium Bacteria Microbiology Gram staining