04/29/2013 G-Unknown code Gram Positive-Micrococcus luteus Gram Negative- Klebsiella pneumoniae Unknown Paper The unknown project was a very good realization of me and my partner going out by our selves. The very first day of the Unknown project I was acquainted with Denise who was very friendly and was very nice as far as assisting me in the project. The first day we were introduced too various different forms of the unknown such as broth‚ Blood agar plate‚ MSA plate
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Intro to Microbiology: Gram stain: Gram + Gram - Exotoxin Except Listeria has endotoxin Endotoxin Except Capsules Strep pneumo VERY Dangerous: (Some Strange Killers Have Pretty Nice Capsules) 1. Salmonella 2. Strep 3. Kleibsiella 4. Hemophilus B 5. Pseudomonas 6. Nisseria-(largest capsule- most likely cause of any toxicity) 7. Citroacter Gram (+) Gram (-) N-acetyl muramic acid (Nam) + N-acetyl glucosamine (Nag) = Peptidoglycan wall Contains
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Dalandan (Citrus aurantium) as alternative decolorizers in Gram Staining bacteria. Kamias (Averrhoa bilimbi) has a high oxalic acid content‚ fruit used to remove stains from clothing and for washing hands‚ removing rust and stains from metal blades. Dalandan (Citrus aurantium) is an aromatic variety of citrus that produces highly bitter‚ acidic fruits. Escherichia coli‚ a gram-negative bacillus that will appear as blue or violet when Gram stained‚ because it has a thick cell wall that prevents the
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differences between the techniques‚ to able to read results and to have the knowledge for future use in various fields. -Simple stain helps with determining the sizes‚ shapes and arrangements of microorganisms. -Gram stain assists in identifying the type of microbes‚ whether they are gram positive or negative‚ which in turn tells us what kind of cell wall the microbes have. -Endospore stain tells whether microorganisms make endospores. -Acid-fast stain is helpful in recognizing the existence
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The conventional method was conducted by placing 2.0641 grams of salicylic acid in a dry 125 mL Erlenmeyer flask. In a fume hood‚ 4 mL of acetic anhydride and 5 drops of concentrated sulfuric acid were added to the flask. The contents were swirled in the flask and then heated for 20 minutes in a hot water bath. After the 20 minutes were up‚ 10 mL of cold water were added in 1 mL increments. The flask was cooled for 10 minutes and occasionally stirred. The crystals that formed were collected via filtration
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that inhabit the upper respiratory tract. Haemophilus species‚ Bordetella species‚ and Corynebacteria diphtheria are common causative agents of upper respiratory illness and served as a lead for this investigation. Corynebacterium diphtheriae is a Gram (+)‚ non-sporing‚ non-motile thin bacilli that can replicate without the invasion of deeper tissues. The agent secretes a powerful exotoxin that has the ability to break the epithelial barrier and travel to the heart which can result in myocarditis
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my unknown identification was to carry out a gram stain to observe the cell shape and arrangement. My unknown turned out to be gram positive bacteria cocci shaped with long chain cell arrangement. The unknown was gram positive because the cells stained blue-violet ‚this indicate that my unknown has ability to retain crystal violet-iodine after alcohol application on the cells which is a gram positive characteristic caused by more peptidoglycan than gram negative organisms on cell walls . To observe
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Exercise 5 Introduction: Many different species of bacteria look similar under the microscope and also have the same staining results (ex. Gram stain). To be able to differentiate between the different species‚ one can look at the metabolic differences (fermentation)‚ as well as the environmental condition differences (temperature‚ pH‚ oxygen requirements). Being able to manipulate these conditions in a controlled environment can help to correctly identify the exact bacteria. Different media
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cross walls). Some have slow gliding locomotion. (Rainis 43) When looking at a bacterial sample under a microscope‚ the experimenter has to stain the bacteria first. The stain serves to define the bacterial cells. All bacteria are either gram negative or gram positive. To stain a sample first the scientist has to “set” the sample. Setting the sample is the basis for all stains. To “set” or simple-stain a sample of
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ASEPTIC TECHNIQUES AND CELL COUNTING INTRODUCTION:- Microbes are single celled organisms and are so small that we cannot see with our naked eyes‚ microbes can only seen under microscopes .Microbes are the one of the oldest living form on earth‚ fossils of microbes were found which are said to be 3.5 billion years old . Microbe world: what is a microbe [online].[Accessed 9 April 2013].Available from: world wide web:‚microbes are found everywhere in rocks‚air‚water some of the microbes are even
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