Abstract: It is important to be able to identify pathogenic bacteria that may be causing harm. Tomato crops can be affected by several different pathogenic bacteria. By using Koch’s postulates‚ it was determined that Pseudomonas syringae was the bacteria causing rot. There are four criteria that must be met when using Koch’s postulates. They are that the organism must be fund in all infected‚ the organism must be isolated in pure culture then once reinnoculated in a healthy host‚ must cause the
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The tests used to confirm the fermentation were a gas production test‚ pH test‚ Gram stain‚ and turbidity test. The results showed during the conversion of milk to Kefir there was gas produced‚ a decrease in pH‚ more bacteria present‚ and an increase in absorbency. The results proved that fermentation occurred with three positive confirmatory results from the gas production‚ pH‚ and turbidity tests‚ while the Gram stain test acted as a negative confirmatory test. Introduction: The main goal of
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Microscopic observations were performed on four different microscope slides. Three of these observations were gram stains and the last observation was of an endospore staining. The unknown bacteria samples for the endospore stain were taken off of a nutrient agar plate and stained using the Bartholomew and Mittwer’s method. Gram stain samples were taken off of tryptic soy agar and stained using the gram stain procedure that is listed in the Willgoh and Bleakly manual (2). Physiological Tests All biochemical
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was correct. The findings are that we were able to identify‚ by process of elimination‚ the four different test bacteria. PROCEDURE - These procedures were followed for all four media test samples. Label test sample. Record Case study and Gram Stain results. For glucose testing: Select red glucose broth with durem tube. Inoculate red glucose media. Incubate for 24 hours. Record results. For lactose testing: Select red lactose broth. Inoculate red lactose media. Incubate for 24 hours
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different biochemical tests for the isolated organisim from the sputum sample‚ it was concluded that the unknown Gram positive organism was Streptococcus pyogenes. The sputum sample was first inoculated in the agar plates MAC‚ SBAP‚ CHOC and also a direct gram stain of the sample was performed. After a day of incubation‚ the MAC agar gave a negative result‚ therefore it is not a Gram-negative bacteria and non-lactose fermenting. The SBAP and CHOC showed growth of colonies. The CHOC agar had a mixed
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Geo-Engineering: Controlling the Climate and Weather Recall the pleasure derived from the last time you took a walk through a park on a day when the weather was perfect. The sunshine was bright‚ the temperature was perfect‚ and perhaps there was a slight breeze that left you the ultimate feeling of refreshment as it caressed your face. Or perhaps you can remember the last time you enjoyed the sounds and smells of a light April shower that left the trees and grass looking so green and the air
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testing in order to determine the identity of the unknown organism. The tests performed to identify the unknown organism included Gram Staining‚ Fluid thioglycollate/aero-tolerance test‚ Methyl Red-Vogues Proskauer‚ SIM test‚ Lysine Decarboxylase test‚ Lactose Fermentation test‚ Simmons Citrate test‚ and TSI test. The Gram staining technique showed that the organism was Gram negative and bacilli shaped. The fluid thioglycollate test showed that the organism was a facultative anaerobe‚ meaning ATP can
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identified and one result was inconclusive. The focus of this paper will be on the description of the just one of the three bacteria and some of its effects on humans. The first test done was a differential stain (gram stain) to determine the unknown microorganism cell shape and arrangement. The gram staining is done by first putting a sample of the unknown bacteria on a slide and it was heat fixed taking care not to over or under fixed the slide. The slide was flooded with crystal violet and let stand for
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Nyaya Panchayats Government proposes to establish Nyaya Panchayats in every Gram Panchayat or a cluster of Gram Panchayats‚ for providing a system of fair and speedy justice‚ both civil and criminal‚ to the citizens at their doorsteps‚ outside the formal judicial system. The Nyaya Panchayats are proposed to be constituted through the election of the Nyaya Panchas by people residing in the area to which the jurisdiction of the Nyaya Panchayats extends. Provisions are proposed for the reservation
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single bacterial colony from the culture. The remaining 3 parts will be conducted on that colony. In part (b)‚ it is shown that various staining techniques test for different characteristics. As the name suggests‚ a gram stain is conducted to identify the bacteria as gram negative or gram positive. Two other stains were carried out. To determine the motility of the bacterium‚ wet mounts of the bacterium were observed and the motility was confirmed by using soft agar plates and soft agar deeps for
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