PART ONE: GENERAL BACTERIA IDENTIFICATION Review the dichotomous key in Figure A‚ the bacterial shapes in Figure B‚ and the Gram stain information below. You will use all three to determine to which major group unknown bacteria belong. Gram Stain Results: Purple = Gram positive Red = Gram negative Neither purple nor red = No cell wall (neither Gram positive nor Gram negative) Review Figure C on the following page. Use Figure C to identify the type of arrangement displayed by the unknown
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positive staining. (3 marks) Positive staining is done by staining the cell walls of Gram-positive bacteria with crystal violet. The cell wall of Gram-positive bacteria is made up of a thick layer of peptidoglycan which undergoes dehydration during decolourisation causing the pores to shrink. This shrinkage of pores traps CV-I complex and stain the cell purple. 2. Using a flow chart‚ outline the process of gram staining. (5 marks) Flood the slide with crystal violet for 1 minute then rinse with
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Microbiology Lab Quiz #2 Study Guide Exercise 9 -- The Gram Stain Compare and contrast simple and differential staining techniques. Simple Staining Simple staining is useful in determining the basic morphology of an organism. Simple staining involves only one reagent i.e. crystal violet‚ basic fuchsin‚ or methylene blue. Simple dyes used to stain bacteria have a positive charge cationic (+)‚ therefore‚ they are attracted to the bacteria that are usually negatively charged anionic (-) Depends
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Robert Suran May 1 2013 Microbiology Abstract Over a three week time span I was able to successfully identify an unknown microorganism by combining the results of several tests. The first test was the Gram stain test which showed the microorganism was Gram positive and rod shaped. Using an Unknown Identification Flowchart‚ I proceeded to inoculate a Starch plate. In week 2‚ I analyzed the Starch plate and was able to determine that the results for Starch Hydrolysis were negative
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Unable to read the Gram Stain at first it was later confirmed that Unknown #7 was a gram negative bacteria. When introduced to Starch Unknown #7 produced no zone of clearing showing that it did not contain the enzyme amylase. When introduced to the Lipid Unknown #7 did produce a zone of clearing indicating that it did contain the enzyme lipase. When introduced to Casein Unknown #7 produced no zone of clearing indicating that it did not contain the enzyme protease. When streaked onto the Gelatin‚
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Staining Gram Staining and Acid Fast Staining Introduction: Differential Staining‚ one which facilitates differentiation of various elements in a specimen is a general term that can refer to a number of specific processes. Using multiple stains can better differentiate between different microorganisms or cellular components of a single organism. Gram’s Stain is a widely used method of staining bacteria as an aid to their identification. It was originally devised by Hans Christian Joachim Gram‚ a Danish
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types of staining through which the bacteria can be identified based on the color and shape. The staining methods used in the lab are Gram Staining‚ Capsule Staining‚ Endospore Staining‚ and Acid Fast staining. One of the most significant method of staining is the Gram Staining‚ as it is highly dependent (McCarthy‚ 25). In the specific experiment that was done‚ Gram Staining was used and the bacteria that was found was purple and round (cocci) shaped. Through this the bacteria was identified as Staphylococcus
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physiological class of bacteria capable of utilising agar as a sole carbon source. This ability is made available by the use of agarases - enzymes which break down agarose into oligosaccharides. This physiological class branches through genii‚ regardless of Gram Stain status or morphology. Through a review of scientific literature we can find identification methods‚ optimum conditions and the general function and location of agarolytic bacteria‚ as well as methods to culture them in vitro for study and experimentation
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test the purity of the unknown. After the plates were incubated and grown‚ the morphology was observed and recorded and a Gram stain was performed. After determining the Gram reaction‚ specific biochemical tests were performed. The biochemical tests were chosen from the unknown identification tables that were in the lab manual. Since the unknown #54 was determined to be a Gram positive rod‚ a thyoglycollate test was performed to determine aerobic activity. Table 1 lists the test‚ purpose‚ reagents
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POLHN Microbiology Student Name: SOKOPETI FAKAOSIFOLAU. Assessment 6 – Gram negative bacilli 1. Which of the following is not true of Enterobacteriaceae? a. Gram negative bacilli b. Oxidase positive c. Reduce Nitrate to Nitrite d. Ferment glucose with acid production 2. Which of the following organisms does not belong to the Enterobacteriaceae group? a. Shigella flexneri b. Eschericia coli c. Proteus mirabilis d. Pseudomonas aeruginosa 3. Name the four species of Shigella
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