LAB REPORT NUMBER TWO DATE: 3/25/2010 inal attachment Lab Experiment number 11 PURPOSE: To learn the Gram stain technique‚ the reason for the stain‚ and how to identify the results of the organisms stained. MATERIALS: Bunsen burner‚ inoculating loop‚ staining tray‚ glass slides‚ bibulous paper‚ lens paper‚ oil‚ and microscope METHODS: Apply Crystal Violet (Primary stain) for 1 minute. Rinse with D-water Apply Iodine (Mordant) for 1 minute. Rinse with D-water. Apply Alcohol (Decolorize) for
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the knowledge gained from the sessions in the laboratory‚ we can now integrate what we have learned to the process of finding out the unknowns given. Materials and Methods The professor gave out the unknown specimens. It contained one-‐gram positive and one-‐gram negative bacteria from the given list. I was assigned unknown A. The process of identification was achieved by utilizing procedures learnt during the semester. Procedures were followed as stated in the lab manual (1). Since the sample contained
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The Demographic Transition One attempt to summarize an observed voluntary relationship between population growth and economic development is the demographic transition model. It traces the changing levels of human fertility and mortality presumably associated with industrialization and urbanization. The first stage of that replacement process-and of the demographic transition model-is characterized by high birth and high but fluctuating death rates. As long as births only slightly exceed deaths
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Geology 101 Complete Final Exam Study Guide All Questions Answered Fall 2012 Chapter 1 – Earth in Context Understand the workings behind the scientific method. What are the different steps and the balances and checks associated with the scientific method. Recognizing the problem Collecting data Proposing hypotheses Test hypotheses Form theory The Earth began to differentiate forming a stratified Earth. How does density change with depth? What are these zones (not necessary to
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GEICO Culture: Nicely’s approach to the Four Functions of Management In the mid-1930s‚ at the height of the Great Depression‚ there weren’t many people with the foresight and courage to start up a new company. Yet the husband and wife team of Leo and Lillian Goodwin were up to the challenge. Confident that he could create a successful auto insurance business by marketing directly to carefully targeted customer groups‚ Leo Goodwin hammered out a business plan during his early career in Texas.
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Goule Selected as New NAWG CEO Washington‚ DC - The National Association of Wheat Growers (NAWG) announced today the selection of Chandler Goule as its new Chief Executive Officer. Goule‚ currently Senior Vice President of Programs at the National Farmers Union (NFU)‚ comes to NAWG with eleven years of agriculture policy experience on the House side of Capitol Hill and will assume the role of Chief Executive Officer beginning July 5. NAWG has been conducting a nation-wide search for a new Chief
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Unknown Organism Report Unknown Organism # 4 Klebsiella pneumonia Klebsiella pneumonia has a rod gram stain with the agar slant cultural characteristics being slimy‚ white‚ somewhat translucent‚ raised growth. It is small gram-negative coccobacilli that can cause lobar pneumonia. The litmus milk reaction is acid‚ gas‚ and curd with a variable reaction. Klebsiella can grow either in the presence or absence of oxygen because of its nature of being a facultative anaerobe. Oxygen is a preferred energy
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Bacterial Smears Are Fixed before Staining to? Answer It is important to heat fix the bacterial smear before staining so as to‚ kill the bacteria‚ firmly adhere the smear on to the microscopic slide to prevent washing off during staining‚ and to allow the sample to readily take up the stain. Reference: www2.hendrix.edu What is the purpose of heat- fixing the smear? It helps the cells adhere to the slide so that they can be stained. The purpose of heat fixing is to kill the organisms without
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Stainin g What is Cellular Staining? Cell staining is a technique that can be used to better visualize cells and cell components under a microscope. By using different stains‚ one can preferentially stain certain cell components‚ such as a nucleus or a cell wall‚ or the entire cell. Most stains can be used on fixed‚ or non-living cells‚ while only some can be used on living cells; some stains can be used on either living is or non-living What Cellularcells. Staining? The most basic reason that
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#3. Name the types of microscopes. Simple Microscopes Compound Microscopes Scanning Electron Microscope Transmission Electron Microscope #4. Identify these stains: (Functions and Reagents) Simple Stain- CULTURES: Staphylococcus epidermis slant Bacillus megaterium broth MATERIALS USED: Methylene blue‚ Distilled water‚ Slide‚ Inoculating Loop FUNCTION: To observe shape size‚ morphology‚ and arrangement. ---FROM SOLID: (slant) 1. Mark the smears on the underside
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