STAINING FOR BACTERIAL ISOLATION Submitted by: Cherry Luz L. Rezaga Submitted to: Dr. Tomas J. Fernandez Jr. CRITERIA OF REPORTING 1. Thorough research (20%) 1.1. Number of literature cited more than five (5) 1.2. Completeness of the topic (5) 1.3. Principle discussed (5) 1.4. Literature should be cited in the text (5) 2. Confidence (20%) 2.1. Eye to eye contact (5) 2.2. No reading (5) 2.3. Proper handling of visual aids (5) 2.4. Magnified voice (5) 3. Appropriateness of visual
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were notified under the NREGA with effect from April 1‚ 2008. Thus NREGA covers the entire country with the exception of districts that have a 100% urban population Key Stakeholders of NREGA comprises of Wage seekers‚ Gram Sabha‚ Panchayati Raj Institutions (specially the gram panchayat)‚ Programme Officer at the block level‚ District Programme Coordinator‚ State Government & Ministry of Rural Development. During the first six months of the financial year 2011-12 as per reports available with
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inoculating loop or needle‚ Bunsen burner‚ glass slides‚ lens paper‚ bibulous paper‚ regular tap water‚ along with reagents Crystal violet‚ Gram’s Iodine‚ 95% ethyl alcohol‚ and safranin. The first test I conducted was the gram stain. In preparation for the actual gram staining process I had to make a smear. To do this‚ I acquired a clean glass slide. I then placed one drop of water onto the center of the slide. Using the flame from my Bunsen burner I sterilized my loop by passing the malleable
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stain 1995 Del Mundo‚ Layah Basella rubra as a biological stain Experimental The alugbati extract turned out to be favorable stain for plant nuclei and organelles especially for Hydrilla verticillata and as substitute for crystal violet or safranin in Gram staining. The extract stained the bacteria red-orange and the plant nuclei‚ and the cytoplasm red and light pink‚ respectively. The study is limited to the ability of Basella rubra (alugbati) to be used as a biological stain 2011 Gel-O-Fury- Saavedra
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Lab Practical One Report Unknown Bacteria Staining Gram Acid Fast Spore The first practical for microbiology was staining of an unknown bacteria. Professor Goode provided an unlabeled bacteria in which each individual were to conduct gram‚ acid fast‚ and spore staining tests from memory. When the gram staining test was conducted a positive results was obtained. When views on oil bacteria appeared purple and rod-like. I noted this result and looked at the lab key to see what the results narrowed
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As a result of the tests ran‚ I was able to identify the unknown bacteria. For Unknown A‚ I ran six tests. I first isolated the bacterium from the second bacterium and found a clear growth (Table 1‚ Figure 1). Secondly‚ I ran a gram stain and found a gram positive‚ cocci bacterium (Table 1‚ Figure 2). Third‚ I ran a catalase test in which was negative (Table 1). From here‚ I determined a starch hydrolysis test would be necessary to distinguish between different bacteria. The result was negative (Table
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patterns. I chose to test a smaller‚ red colony for my experiments. Based on its gram reaction and oxygen requirements‚ the following tests were performed to reach my presumptive ID: Test Performed Result Gram Stain + Fluid Thioglycollate Strict aerobe Catalase + Nitrate Reduction + Growth 5% NaCl - Growth 7.5% NaCl + Gelatin Hydrolysis - Glucose Fermentation + My organism appeared to consist of gram-positive cocci‚ making it a member of Group 17 in Bergy’s
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Review for Microbiology Test #1 (Lesson 1-5) Lesson 1 What is the #1 killer of man worldwide? Heart Disease. What is the #3 killer in the US? Infectious Disease. What is the importance of MO in our world? List 6-8 reasons MO are important. Can’t live in Germ Free World‚ Keeps Economy running‚ Agriculture‚ Medication‚ Baking‚ Cosmetics‚ Paints‚ Fertilizers‚ Helps develop immune system‚ Decomposition of dead plants and animals to enrich the soil. What are the 4 groups of people most prone to
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getting the supplies in order for the experiments that I found much difficulty in. Questions A. List three reasons why you might choose to stain a particular slide rather than view it as a wet-mount. Gram stains provide a more intricate view of shape Gram stains provide better views of structure. Gram stains show changes in bacteria. B. Define the following terms: Chromophore: It is part of the molecule that makes color when light is passed through certain wavelengths Acidic dye: They are soluble
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was in our number seven vial would become a battle we were willing to take on. As I began the test of deciding if our little bacteria friend was gram positive or negative‚ Jordan my science teammate‚ was putting together a smear plate. In as little as ten minutes we had discovered by the pink oval shapes we were observing‚ our microbe friend was a gram negative rod. We had narrowed our search down to five! We decided next we would do oxygen along with a motility test. Along with those‚ we did a fermentation
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