I was able to come to the conclusion that my unknown is Klebsiella pneumoniae through the use of Bergey’s Manual. After performing a gram stain of my unknown I was able to determine that my unknown was a gram-negative rod. While testing the oxygen requirements my test tube had growth throughout the tube‚ indicating my unknown was a facultative anaerobe‚ which can grow in the presence or absence of oxygen. The most important test was whether my unknown was motile or nonmotile. My test tube only showed
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partner and I were given unknown number 3 in the laboratory. After performing various tests over the course of a few weeks on our unknown‚ we came to the conclusion that our unknown organism is Klebsiella pneumoniae (K. pneumoniae). K. pneumoniae is a gram negative bacillus shaped microorganism. We observed that K. pneumoniae is a nonmotile organism. We performed multiple tests on our unknown culture‚ therefore we are very confident that it is correctly identified. We identified that K. pneumoniae
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most important tests done was gram staining because it helped to limit the possible bacterium that it could have been. To be sure which bacterium was worked with we referred to the Bergey’s manual and compared the results to the possible bacterium. Results:
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GEO 100 – Environmental Geology - Final Exam Multiple Choice 1. Most astronomers believe that the universe a. has existed in its present state since the beginning of time. b. formed from a huge rotating cloud of gas. c. came into existence as the result of a “Big Bang.” d. is no more than five billion years old. 2. Most of the mass of the solar system is concentrated in a. Jupiter and Saturn. b. Earth. c. the planets. d. the sun. 3. The solar system formed about a. 5 million years ago. b. 4.5 billion
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for each of the unknown microorganisms. After both plates were properly inoculated and incubated at 37C for 24- 48 hours‚ and two separate morphologies were then observed and recorded‚ unknown A and unknown B‚ a Gram stain was then performed for each unknown. After determining the Gram reaction‚ alternate staining procedures were performed as needed. Unknown A required further endospore staining‚ whereas unknown B required no further staining procedures. Once all staining methods were complete
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(TLC) and consistent with its IR & 1HNMR spectra. The synthesized analogue was screened for antibacterial activity against one gram positive & two gram negative species. The compound exhibited good antibacterial effect towards gram negative species when compared to the standard ciprofloxacin. At the same time the analogue was retaining antibacterial activity towards gram positive species when compared to standard ciprofloxacin. The molecular docking studies showed a good correlation between their
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Differential Staining Questions: A. What is a differential stain? How is it different from a simple stain? 1. A differential stain is a stain that differentiates between gram-positive and gram-negative bacteria. Bacteria that stain purple are considered gram-positive and those that stain pink are considered gram-negative. 2. A simple stain is a stain that reacts with all microbes in and identical fashion. They are used to increase contrast. 3. They are different from one another because
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Gram Characteristics of Bacteria Introduction Bacteria are interesting little critters in that they are not easily visualized without some sort of stain. In classical methodology‚ two different kinds of stains have been used to identify bacteria: acidic and basic dyes. Acidic dyes are so called because they consist of a salt that has a cation that transfers no color‚ but the anion portion is colored and does give off color. Basic dyes consist of an anion that does not give off any color and a cation
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results‚ the isolated bacteria were tentatively identified as Agrobacterium strains. B.Characterization of Agrobacterium tumefaciens: Biochemical tests: biochemical features of the selected isolates were given in the table 1. The Gram reaction indicates selected isolated are Gram negative. All the six isolated are positive
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or clusters and form distinct colonies. Bacteria are classified on the basis of many characteristics. Morphological and physiological features such as cell shape‚ motility‚ formation of spores and other distinguishable structures‚ and reaction to Gram stain is a good start in identifying bacteria. Other staining techniques such as Acid Fast stain are also useful in determining species. More important in identification of a genus and species of bacteria are biological tests‚ including the determination
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