The Results from the Gram positive tests indicated that the unknown #4 was Streptococcus pyogenes. All seven tests on the unknown matched S. pyogenes perfectly. The blood agar plate proved the unknown to be β hemolytic‚ meaning the unknown bacteria was capable of complete hemolysis. This test separated the unknown into the β Streptococcus group‚ narrowing the possible bacteria to S. aureus or S. pyogenes. The Catalase test was used to determine if the unknown could break down hydrogen peroxide
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Antimicrobial Activity of Cilantro on Staphylococcus Epidermis and Escherichia coli Abstract Antimicrobial effects of cilantro against the gram positive bacteria S. epidermis and the gram negative bacteria E. coli were looked at. We predicted that cilantro would act as an antibiotic against S. epidermis but not to E. coli because S. epidermis does not contain an outer membrane whereas E coli. does. We predicted that the semi-permeable outer membrane of E. coli would protect the bacteria from
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the cell wall synthesis promoting osmotic instability‚ usually bactericidal. It hinders or kills bacteria. Ceftriaxone sodium is a broad-spectrum bactericidal cephalosporin antibiotic. Ceftriaxone is active in vitro against a wide range of Gram-positive and Gram-negative organisms‚ which include β-lactamase producing strains. Ceftriaxone is indicated in the treatment of the following infections either before the infecting organism has been identified or when known to be caused by bacteria of established
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antibiotic that works on gram negative bacteria. Gram-negative bacteria are bacteria that do not retain crystal violet dye in the Gram staining protocol whereas Gram-positive bacteria are those that are stained dark blue or violet by Gram staining. The bacteria we used were E-coli that are a gram negative bacteria and the only antibiotic out of Penicillin and Streptomycin that will work is Streptomycin. Penicillin is also effective antibiotic but it will only work on bacteria that Is gram positive because
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‚ (2016) in vitro antimicrobial activity of Sudanese Cucumis melo L. fixed oil was investigated. Agar cup diffusion assay was performed against two fungal species (Candida albicans and Aspergillus niger)‚ two gram positive bacteria (Staphylococcus aureus and Bacillus subtilis) and two gram negative bacteria (Escherichia coli and Pseudomonas aeruginosa). Ampicillin‚ gentamycin and clotrimazole were used as positive controls. Antimicrobial activity was shown by all the species except E.coli and P.
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Escherichia coli and Staphylococcus aureus. The effectiveness of the chemical agent was determined by the size of the zone of inhibition around the disc incubated on soft nutrient agar seeded with the two bacteria on separate plates. For E. coli‚ a gram negative organism‚ the zone of inhibition was the largest with Lysol (0.2mm). Next‚ Scope has slightly smaller zone of inhibition‚ 0.15mm‚ on the organism. Hydrogen peroxide‚ Wescodyne and 70 percent Ethyl Alcohol had no affect on the bacteria. Inhibition
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DIFFERENT TYPES OF AGAR Mac Conkey‘s Agar plate MacConkey (also McConkey) agar is a culture medium designed to grow Gram-negative bacteria and stain them for lactose fermentation. It contains bile salts (to inhibit most Grampositive bacteria‚ except Enterococcus and some species of Staphylococcus)‚ crystal violet dye (which also inhibits certain Gram-positive bacteria)‚ neutral red dye (which stains microbes fermenting lactose)‚ lactose and peptone. QUALITY CONTROL Results after 24 hrs at
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colonies that are circular and moist. Microbes A are gram-negative cocci. B had moderate growth of yellow punctiform colonies that are circular and moist. Microbes B cells are gram-positive cocci. C had abundant 2mm growth of pink colonies that are circular and have a mucoid consistency. Microbes C are gram-negative rods bacilli. D had abundant growth of 2mm yellow colonies that are circular and have a mucoid consistency. Microbes D are gram-negative bacilli that gave off a fishy smell. Swarming
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IMPORTANT BACTERIA | GRAM-POSITIVE BACTERIA * Alpha and gamma hemolysis on blood agar * Bacillus cereus colonies on blood agar * Beta-hemolysis on blood agar (S.aureus) * Beta-hemolysis on blood agar * Beta-hemolytic colonies on blood agar * Clostridium perfringens Gram stain * Corynebacterium diphtheriae Gram stain * Corynebacterium pseudotuberculosis on blood agar * Enterococcus faecalis * Enterococcus faecalis on blood agar * Enterococcus faecalis Gram stain * Enterococcus
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In general‚ members of the genus Vibrio are Gram-negative‚ motile and most of the members have a single polar flagellum‚ when grown in liquid medium (Kaysner & DePaola‚ 2004). Vibrio cholerae lives naturally in riverine and is the causative agent of cholera outbreaks and epidemics. Vibrio cholerae is comma-shaped. Initial isolates are slightly curved‚ whereas they can appear as straight rods upon laboratory culturing. Vibrio cholerae is a facultative anaerobe and can undergo respiratory and fermentative
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