WESTERN BLOTTING (Written Report) I. PRINCIPLE Western blotting is a method for identifying a specific protein in a complex mixture and simultaneously determining its molecular weight. In Western blotting‚ proteins are electrophoresed into a gel‚ as the proteins migrate through the gel they are separated based upon size and charge. Characteristically‚ smaller proteins migrate through the gel faster than larger proteins. Sufficiently separated proteins in an SDS-PAGE can be transferred to a
Premium Western blot Molecular biology Gel electrophoresis
Nagib Gonzalez World Civ Section F: The east and west differences emerged from the Greco-Persian war. The idea was that the east stood for despotism and that the west stood for freedom. The eastern and Western conceptualizations still exist but the idea of the East and West in a literal sense is a thing of the past. Today‚ both the Western and Eastern Civilizations influence each other heavily. Western civilization can be compared to modern day Capitalism and the Eastern Civilization can be considered
Premium Asia Western culture Orientalism
LaNeisha Hamilton World War II through 1970’s Hist 105 Professor Stayer March 3‚ 2013 In this paper I will go into details explains two major historical turning points that occurred during the period of World War II through the 1970’s‚ and the effect it had on today’s society‚ economy‚ politics and culture. In this paper I will also give two reasons why in the late 30’s Americans wanted to remain out of the European conflict. In this paper I will talk about the role women played
Premium Lyndon B. Johnson Richard Nixon World War II
THE COLD WAR NUCLEAR WEAPONS OF THE 1980 ’S The pope quickly organized a meeting to prepare the world for a weapon that would destroy all life on earth. "Pope Innocent II organized the conference in 11391" because of a crossbow. Approximately 800 years from this conference‚ the Cold War has begun. The potential of mass destruction could occur at any moment. More efforts for mining and technology went toward constructing nuclear weapons. Missiles‚ such as‚ the Tomahawk® Cruise Missile and
Premium Nuclear weapon Cold War Plutonium
Ana Decastro Western Civilization I Professor Sarah M. Lane The crusades were religious wars promoted by the Catholic Church to regain entrance in holy places in Jerusalem in the thirteenth century. Both‚ Muslims and Jews had similar opinions about the crusades differing from the Christians who approved of them. Pope Urban‚ organizer of the papal monarchy and the Roman Curia‚ was a faithful believer of the crusades. At his speech in Clermont in 1095‚ he warned Europeans to accept the crusades
Free Crusades Christianity Israel
Writing about Literature Reading literature like Beowulf and Emerson’s "Heroism" is just one stop along the way to becoming an English scholar. Writing about literature is a third essential element. In the assignment for this lesson‚ you will synthesize the texts you have read to write a short essay; this means you will combine details from Beowulf and "Heroism" to show your understanding. When writing about what you’ve read‚ you need to consider the general idea of the text and the specific
Premium Sentence Phrase Question
2013 Richard Parker Analysis Being in unfamiliar surroundings after a tragedy can really take a toll on a person. Learning to cope with it could mean bottling up your feelings‚ becoming angry‚ or in Piscine Patel’s case befriending a four hundred and fifty pound Bengal tiger. Depending on which of Pi’s stories you believe‚ Richard Parker is either a real tiger or he’s simply a very developed figment of Pi’s imagination. After analyzing the stories‚ it is clear that Richard Parker was simply
Premium English-language films Religion Psychology
Justin Wannop Mrs. Kinley Language Arts 9 December 21st ‚ 2012 Richard Parkers attributions to Pi’s Survival Life of Pi is an intriguing story about a young indian boy named Pi‚ who embarks on an incredible journey across the Pacific ocean from India to Canada on a lifeboat. On his adventure‚ Pi is forced to confront and overcome the most daunting of obstacles and face some of the toughest survival tasks‚ all while accompanied by a 450 pound bengal tiger. His perspective of the tiger changes
Premium The Narrative of Arthur Gordon Pym of Nantucket Pacific Ocean Tiger
The Western Evils The infiltration of western ideals into the Japanese Culture had forever changed customs and traditions of the Japanese society as a whole. Yet was it as it was stated in a 1941 pamphlet issued by the Japanese Ministry of Education entitled “The Way of the Subjects.” “this country has been widely seeking knowledge in the world since the Meiji Restoration‚ thereby fostering and maintaining the prosperity of the state. With the influx of European and American culture in this
Premium Japan Sociology Meiji period
Effect Of Western Culture On Indian Youth By Moderator .. Published 04/3/2006 GD TOPICS WITH ANSWERS Rating: ” Effect Of Western Culture On Indian Youth " Replies: Posted By: pavani How there is head and tail for a coin‚ there is both positive and negative impact of western culture on India and especially on Indian youth‚ In past in India men were our traditional dresses‚ but now it is entirely changed‚ now the Indian youth moving with jeans‚ t-shirts‚ minis‚ micros
Free Western culture Culture Family