Purpose: To find out the densities and to find out the name of the unknown metals. (Based on the extensive and intensive properties) Check up the words mass‚ volume‚ density‚ extensive properties‚ and intensive properties. Where do the units for mass and volume) come from and what do they mean? What is the density of distilled water? What is Archimedes principle? Does temperature affect the density of a solid? Liquid? Gas? Materials: Safety glasses‚ 10‚ 25 or 50 mL graduated cylinders
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In this lab‚ we performed a genetic transformation through the process of gene transfer. Gene transfer involves the insertion of a gene into an organism. The gene to be inserted is usually contained in a plasmid‚ which is relatively small‚ circular non-chromosomal DNA molecule typically found in bacteria. Once the plasmid containing the gene is inserted into the organism‚ it is absorbed into the organism’s own genetic code. After this occurs‚ the newly introduced gene begins coding for proteins‚
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IB SL Biology Lab Molecular Biology: Transformation and Electrophoresis Christina Qi 2/16/07 Aim: How can a plasmid be engineered to include a foreign piece of DNA and how does gel electrophoresis separate DNA molecules present in a mixture? Hypothesis: If the pGLO plasmid is inserted into competent Escherichia coli cells‚ then the transformed bacteria will be resistant to ampicillin and will glow green under UV light. If samples of DNA are cut using certain restriction
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Stacy Hernandez Period 1 Mrs. Riley AP Biology Osmosis and Diffusion Lab I. Introduction: Diffusion is vital to many life functions of a cell‚ it allow the transportation of vitally important nutrients and compounds without the expenditure of excess metabolic energy. To explain diffusion‚ it is as if a bottle of perfume is opened at one end of the room‚ then in a short amount of time a person at the other end of the room can detect the scent of the perfume; this is the process of diffusion
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Expression and Purification of Human Platelet-Derived Growth Factor Beta (PDGF-B) Rachel C. Hermina-Stewart* and Elsie I. Parés-Matos† *Industrial Biotechnology Program‚ †Department of Chemistry University of Puerto Rico-Mayagüez Campus rachel.hermina@upr.edu December 14‚ 2009 ABSTRACT. Platelet-Derived Growth Factor (PDGF) is one of many proteins that regulate cellular growth and division. The PDGF family consists mainly of five different isoforms called as PDGF-A‚ PDGF-B‚ PDGF-C and PDGF-D. Three
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well their ability to be modified to express proteins of interest. A pGLO plasmid contains genes for the green florescent protein (GFP) as well as the gene for ampicillin resistance known as beta-lactamase. It also contains a gene regulation system (operon) that has the ability to control expression of the GFP gene in transformed cells known as araC. The source of GFP is naturally founds within a
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Introduction: The method for purifying solids is done by observing the melting point. This is important because if there is an impure substance the melting point would be depression and the melting range would be broader. By finding the melting point of a known substance one can then observe an unknown substance’s melting point to see if the points are the same and try to figure out the unknown. Experimental Section: The first known substance we used was Biphenyl. 68 degrees Celsius is the
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Experiment #4A- A Separation and purification Scheme Amena Alkeswani‚ PHCC Dhanlal Murally‚ PHCC September 27‚ 2012 Abstract: There are millions of different organic compounds. Most of them are found in mixtures and in order to achieve a pure form they need to be separated‚ isolated‚ and purified. However‚ there are endless numbers of possible mixtures‚ which make it impossible to have a pre-designed procedure for every mixture. So chemists often have to make their own procedures. The purpose
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African Diaspora and the World After reading the short story “Diaspora” by Joanne Hyppolite‚ I got a vivid understanding of how diaspora can have an effect on one’s identity. Over the past month we have studied many theories and concepts concerning race‚ gender‚ and politics. There are three theories that capture the essence of Joanne Hyppolite’s worldview as a Haitian growing up in America: intersectionality‚ identity‚ and diaspora. Individuals oftentimes experience the theory of Intersectionality
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University of Texas at Tyler Lab 3C: Purification of L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally
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