Title of the lab: Transformation : Bacterial Genetics Purpose of the lab: The pupose of the lab was to transfor a bacterial E. Coli by using the green flurescent protein from the jellyfish. Another important that was fferdone by making the cell competency‚ meaning that it will be able to take on additional DNA. This was done when the plasma was added. Materials: 1. 37 o C water bath 2. Ice 3. Sterile transfer pipette 4. Foam tube rack 5. Transformation solution (CaCl2) 6. pGLO plasmid 7. Sterile
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will broaden range &may increase melting point Lab Questions 1. Define the following A. Melting point B. Intermolecular forces C. Hydrogen bonding 2. Arrange the following IMF according to there strength Dipole-Dipole interaction forces‚ Hydrogen bonding‚ Van der Waals forces 3. How does the atmospheric pressure affect the melting point? 4. What is the purpose of determining the melting point in this lab? 5. Why is this method not used for finding the melting
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introduction of new genetic information which is merged into the organism’s genome. Bacterial transformation is a type of genetic transformation that was used in lab and mainly used due to the single celled nature of bacteria. In this lab‚ the engineered pGLO plasmid is integrated into E. Coli bacteria‚ and adds the genes which code for the proteins GFP in the modified bacteria’s genome (Hanahan‚ Studies on transformation of Escherichia coli with plasmids‚ 1983). To see the reaction of this plasmid on the
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Back Laboratory Program SAFETY IN THE CHEMISTRY LABORATORY 786 Pre-Lab Extraction and Filtration 790 1-1 Mixture Separation 792 1-2 Water Purification 794 3-1 Conservation of Mass 798 4-1 Flame Tests 801 Pre-Lab Gravimetric Analysis 828 13-1 Separation of Pen Inks by Paper Chromatography 830 13-2 Colorimetry and Molarity 834 14-1 Testing Water 838 804 7-1 Separation of Salts by Fractional Crystallization 806 7-2 Naming Ionic
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1. Why are spindle fibers important for mitosis? (5 points) Spindle fibers work as a path for chromosomes to travel(Campbell‚ 2009). 2. State the four bases that make up DNA. (4 points) a. (A)-Adenine b. (G)-Guanine c. (T)-Thymine d. (C)-Cytosine (Campbell‚ 2009). 3. What are the two base pairs? (2 points) a. AG b. CT 4. Answer the following questions: a. Define the term crossing over. (3 points) The phase in meiosis where chromosomes exchange segments of genetic materials (Campbell‚ 2009). b. Explain
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vesicles and to observe the effects of mutations in nerve transmission in Caenorhabditis elegans. C. elegans is a species of nematode that has a simple nervous system and has synaptic vesicles that can be easily tagged with green fluorescent protein (GFP) and viewed under fluorescence microscopy. The experiment was to observe three strains of C. elegans and determine which of the phenotypes observed showed certain mutations and then do a comparison
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Transformation Lab Report Introduction Transformation is the transfers of virulence from one cell to another‚ through the transferring of genetic material. It was originally postulated in 1928 through the works of Federick Griffith‚ a British microbiologist. Griffith observed that the mutant form‚ non-virulent form‚ of the bacteria Streptococcus Pnumoniae could be transformed into the normal‚ virulent form‚ when injected into mice along with heat killed normal forms. He concluded that somehow
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Writing of Lab Reports __________________________________________________________________________ W hy should I bother writing lab reports in the correct way?” The Foundation Programme is designed to prepare you for undergraduate studies at UTAR which will require the writing of lab reports all years generally. At the end of your third year‚ you may have an opportunity to work on scientific projects which will culminate in an official scientific report. Depending on the quality of your report
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The purpose of lab number thirteen was to examine the response of the daphnia to a chemical stimulant. Also examined was how human resting heart rates differed from the daphnia’s. Stimulants are any substances that raise the levels of activity in the body. Stimulants could be a number of things such as physical or chemical. Physical stimulus usually deal with something that affects the five senses such as sight‚ hearing these could be attributed to loud noises or too much light going into your eye
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Margaret E. Vorndam‚ M.S. Version 42-0038-00-01 Lab Report Assistant This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions‚ diagrams if needed‚ and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information in an editable
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