"Gfp purification lab" Essays and Research Papers

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    LAB REPORT FOR EXPERIMENT #2: PURIFICATION OF ACETANILIDE BY RECRYSTALLIZATION Your name TA’s name Your Partner’s name Lab Section OBSERVATIONS: A. SELECTING A RECRYSTALLIZATION SOLVENT | |Solubility Test (cold) |Solubility Test (hot) | |Water |insoluble |soluble | |pet ether |insoluble

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    oil purification

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    OIL PURIFICATION A SCIENCE INVESTIGATORY PROJECT CHAPTER I Background of the Study INTRODUCTION Oil used in cooking foods is commonly derived from vegetables. Cooking oil is commonly used for frying and oil used for frying is usually thrown away. The recycling of cooking oil also provides a form of revenue for restaurants‚ which are sometimes compensated by cooking oil recyclers for their used deep fryer oil. Cooking oil recycling also results in less used oil being disposed

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    CON 280 GFP PAPER

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    Shayna Purcell Professors Karla Williams and Scott Gilbreth Source Selection and Administration of Service Contracts CON 280 18-043 6 July 2015 Government-Furnished Property (GFP) As a Contract Administrator my job actually deals with government-furnished property often. The definition as used by the FAR is “property in the possession of‚ or directly acquired by‚ the Government and subsequently furnished to the contractor for performance of a contract. Government-furnished property includes

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    DATE:23 RD JANUARY‚2014. TITLE: SEPARATION AND PURIFICATION OF ORGANIC COMPOUNDS AIMS: To isolate organic‚ inorganic and component from a given sample. To become acquainted with various separation methods. To examine the solubility behavior of the various compounds in a mixture using different solvents

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    system for the construction of enhancer trap lines in Arabidopsis. (Lovell‚ 2013) GAL4-GFP enhancer trap system It’s a system for GAL4 targeted gene expression. An enhancer trap vector bearing a modified GAL4-VP16 gene was inserted randomly into the Arabidopsis genome by Agro bacterium mediated transformation. Cell specific activation of the GAL4-VP16 gene by cellular enhancer results in activation of a linked GFP gene‚ allowing simple characterization of expression patterns. Targeted expression of

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    Purification of an Ester

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    Wintergreen". http://www.petrik.com/PUBLIC/library/misc/glossary_of_org_chem.htm#ESTERS Aim To synthesise the ester 3-methylbutyl ethanoate( isopentyl acetate) and to identify the product by its boiling point. Method The ester was prepared by the lab assistant in the fume cupboard by reacting 3-methylbutan-1-ol (iso-pentanol‚ 25mL)‚ and acetic anhydride (24mL plus 3 drops of concentrated sulfuric acid). A strong exothermic reaction occurs. Once the reaction mixture is cooled in the fume cupboard

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    Purification of Infliximab[3] begins at Stage 1 with the filtration of the harvest material using a spin filter. The harvest is then clarified and undergoes 3 main chromatographic steps. Firstly‚ at Stage 2‚ the direct protein capture by Protein A affinity chromatography helps to remove a large majority of the impurities whicn include viruses and media components. At Stage 3‚ prior to cation exchange chromatography‚ the purified material in the eluted stream is then subjected to freezing‚ thawing

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    Experiment 55: A Separation and Purification Scheme The final lab report/procedure write-up will consist of the following: Report guide: 1. List the names‚ structures‚ and amounts of each compound in your mixture. 2. Draw a flow chart (like pg 687 in PLKE) showing how you separated and isolated your two compounds. Be sure to include the structures of the compounds at each step. 3. Write a procedure. This should include exactly how you performed your separation. It should have enough

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    Colegio de San Juan de Letran College of Liberal Arts and Sciences Experiment #3 SEPARATION AND PURIFICATION OF ORGANIC COMPOUNDS: CRYSTALLIZATION Gomez‚ Paola Anne M. DOP: 2 July 2013 Student no. : 4120403 DOS: 9 July 2013 Group: Carcinogenic Remarks: _____________________________________________________________________ Engr. C. D. Sanchez Instructor THEORETICAL DISCUSSION Crystallization is a technique which chemists use to purify solid compounds. It is

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    Experiment B2 Chromatography for Protein Purification Name Matric No. Group : : : Date of Expt. : GRADE : A. Learning objectives 1. 2. 3. 4. Establish chromatographic assay to determine protein concentrations in a mixture. Appreciate the importance of resolution in protein chromatography. Understand the tension between purity and yield in protein chromatography. Understand the importance of mass balance closure in protein purification. B. Introduction I. Fast Protein Liquid Chromatography

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