Student societies by location Belgium Flemish Belgians In Flanders‚ student societies play a unique role in student life. Student societies there have traditionally been politically active‚ and they played a significant part in the 1960s division of the Catholic University of Leuven into separate Flemish and Walloon universities. A student society in Flanders is led by a praesidium. The head of the praesidium (and the society) is the praeses. Alternative spellings are presidium and preses
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Synthesis and Purification of Acetylsalicylic Acid (ASA or Aspirin) Background Salicylic acid is a phenol as well as a carboxylic acid. It can therefore undergo two different types of esterification reactions‚ creating an ester either with the hydroxyl or with the acid. In the presence of acetic anhydride‚ acetylsalicylic acid (aspirin or ASA) is formed. Correspondingly‚ an excess of methanol will form methyl salicylate‚ which is also an analgesic. In this experiment‚ we shall use
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Organic Chemistry I LAB EXAM: FINAL BROMINATION OF BENZENE SYNTHESIS AND PURIFICATION OF BROMOBENZENE: PROCEDURE DATA TABLE Chemical Boiling point C Melting Point C Density g/mL Solubility Benzene 80.1 5.5 0.88 Slightly in H2O Toluene 110.6 -93 0.87 Slightly in H2O Bromobenzene 155-156 -30.8 1.50 Insoluble Dibromobenzene 220.40 87.31 0.96 Insoluble MATERIALS: Graduated cylinder Weight scale Buchner funnel Filter flask Rubber stopper Hot plate Thermometer Conical funnel Various
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Lab #5: Refraction of Light Theory: Refraction can be defined as the bending of a wave when it enters a medium which causes it to have some reduced speed. In terms of light‚ refraction occurs when the ray passes through some medium which slows its speed; such as water or glass. In this instance the ray tends to bend towards the normal of the medium. The amount of bending or refraction which occurs can be calculated using Snell’s Law (). Objective: To measure the index of refraction of Lucite
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BIO-RAD). Objectives a. To understand one of the most commonly used techniques for introducing DNA into E. coli cells and its use in molecular cloning. b. To become familiar with the concept of using green fluorescent protein (GFP) as a molecular tag for studying gene expression in bacteria and other organisms. c. To understand some of the mechanisms by which bacteria regulate gene expression. Introduction Genetic transformation is a process by which competent
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The Purity and Purification of Solids- Part 2- Recrystallization Courtney Percival CH 337 Laboratory‚ Section 037 Department of Chemistry Portland State University‚ Portland OR ABSTRACT INTRODUCTION Recrystallization is a commonly used tool in laboratories when dealing with impure solids. "An effective recrystallization is one in which 1) a high degree of purity is achieved as well as 2) a maximum recovery of sample".1 Solubility‚ the tendency of a substance to dissolve in a liquid‚ is an
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Anytime you can stop a person from lighting up a cigarette‚ whether they resent the fact or not‚ you’re doing them a favor”- (Poland). In Poland’s article‚ Smoking‚ Stigma‚ and the Purification of Public Space‚ he addresses the many problems smokers go through in a day. From public ostracization because of health concerns‚ to restrictions on when and where they can smoke in public spaces. Smokers tend to smoke more often when surrounded by other smokers‚ as well as hide the fact that they smoke the
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Discuss a range of analytical techniques used for the purification of proteins What are proteins? Proteins are the main building blocks of life. They are essential for the body and have many different roles . Proteins are made from sequence of amino acids. Protein structure is determined by their sequence of amino acids‚ which are linked by peptide link. Proteins are made from about 50 to 2000 amino acid residues. Figure 1 Why purify proteins? They are many reasons why a biochemist
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Title: Purification of Green Fluorescent Protein Introduction: Transformation is used to introduce a gene coding for a foreign protein into bacteria. Hydrophobic Interaction Chromatography (HIC) is used to purify the foreign protein. Protein gel electrophoresis is used to check and analyze the pure protein. Research scientists use Green Fluorescent Protein (GFP) as a master or tag to learn about the biology of individual cells and multicultural organisms. This lab introduces a rapid method
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1. Describe the mechanism by which green fluorescent protein (GFP) is able to emit fluorescent light and why ultraviolet light is required to visualise it. (5 marks) When GFP is hit with UV light‚ the chromophore is hit by a photon. This changes the chromophore from ground state (A) to A*‚ which is a highly excitable state. Due to such a highly excitable state not being able to remain so for very long‚ the A* state chromophore emits a proton‚ lowering its state to I*‚ the energetic I. This I* state
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