Toxicology Lab 1. In this investigation‚ a wide range of concentrations of Sodium Chloride (NaCl) solution were created and the effects that they had on radish seeds were tested. This ultimately created a doseresponse experiment in which it was detectable whether or not radish seeds were a reliable bioassay for the toxicity of NaCl. The goal of this experiment was to determine a correlation between toxicity and seed germination/radicle
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Coli bacteria. We began the lab by first obtaining two sterile microcentrifuge tubes‚ one was label “GFP+” while the other one was “GFP–” because this one served as a control meaning nothing goes inside this one. With gloves on 25 microliters competent cells‚ which is e. Coli bacteria compete in calcium chloride and heat shock was
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Lab 5 The Diffraction Grating Chinua McDonald Objective: To measure the wavelength of light with a diffraction grating. Theory: The two types of diffraction gratings are the transmission and reflection gratings. They are made by ruling on a piece of glass or metal a number of evenly spaced lines with a fine diamond point. Diffraction phenomena can be analyzed in terms of Huygens’ principle‚ according to which every point on the wave front of a wave should be considered as a source
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indicated by a very pale pink color. To calculate the molarity of NaOH‚ the following equation was used MNaOH x VNaOH = MKHP x VKHP therefore the molarity was .125 M. INTRODUCTION This lab experiment covers the preparation of standard solution and the acid/base titration. The first part of the lab is to prepare a standard solution of Potassium hydrogen per. A standard solution is a solution of known concentration‚ in which it is prepared using exacting techniques to make sure that the molarity
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Bacterial Transformation Lab Report Backround: The plasmid pGLO contains an antibiotic-resistance gene‚ ampR‚ and the GFP gene is regulated by the control region of the ara operon. Ampicillin is an antibiotic that kills E. coli‚ so if E. coli‚ so if E. coli cells contain the ampicillin-resistance gene‚ the cells can survive exposure to ampicillin since the ampicillin-resistance gene encodes an enzyme that inactivates the antibiotic. Thus‚ transformed E. coli cells containing ampicillin-resistance
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The Expression and Purification of Recombinant Green Fluorescent Protein (rGFP) From E. coli strain‚ BL21(DE3)‚ Using Ni2+-Agarose Affinity Chromatography Abstract: The purpose of these series of experiments was to express and purify recombinant Green Fluorescent Protein (rGFP) from the E. coli strain‚ BL21(DE3) by beginning with its purification via a Ni2+-agarose affinity chromatography column. The His6 tag of the rGFP bound to the Ni2+-agarose column and washes and elutions were obtained
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Constituent of “Panacetin” Recrystallization& Melting-Point Measurement Organic Chemistry Lab Introduction: Panacetin purportedly includes sucrose‚ aspirin‚ and acetaminophen. However‚ the accuracy of Panacetin’s contents has been called into question by the Association for Safe Pharmaceuticals. Therefore‚ the lab must discover the accuracy of the ingredients listed on Panacetin’s label. The unknown in this experiment is presumed to be something
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Oil Precious Ahmed ID# 6463614 Section 07‚ Friday afternoon Lab experiment performed on: Friday‚ January 19‚ 2013 Lab report submitted on: Friday‚ January 24‚ 2013 Introduction Before the synthetic aspirin of today‚ salicylic acid‚ which is the important ingredient found in aspirin‚ was extracted naturally from methyl salicylate found in Wintergreen oil‚ which could be found in certain plants. The purpose of this lab experiment is to prepare salicylic acid from the natural starting material
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Yes 84mL After Sand Separation Brown No Garlic‚ yes No No 67mL After Charcoal Adsorption and Filtration Cloudy Yes Yes No No 61mL Science Concepts & Questions: What percent of the foul water was lost during purification? 39% What volume of water was lost during purification? 39mL Why does the “pure water” conduct electricity and the tap water does not? The “pure water” conducts electricity and the tap water does not because the “pure water” has dissolved such as salt in it. What process
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Moment of Inertia and Rotational Motion Garret Hebert PHY 2311 Tues 1:00 garret.hebert@hindscc.edu Abstract: During this lab we will study what rotational Inertia is and how different shapes of masses and different masses behave inertially when compared to each other. We will specifically study the differences of inertia between a disk and a ring. We will use increasing forces to induce angular acceleration of both a disk and a ring of a certain mass. We will then then measure the differences
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