"Gfp purification lab" Essays and Research Papers

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    Lab for Chemistry

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    Discussion The purpose of the Density Lab was to determine the identity of four unknown solids and two unknown liquids by calculating their densities and comparing them to a density chart‚ taking into account error analysis and finally classifying the substances. In order to calculate this density‚ we first found the mass of the container that was to be holding the substances. We then found the volume of the substance‚ and lastly determined the mass of the container and substance. We subtracted

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    The alchemy lab

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    Shavonna Bailey Barbara Transue English 102A MW 6:30 – 8:40 pm Alchemy The alchemy lab proposed that Alchemy is a craft. The law is that in order to create something new‚ it must die first. You cant be reborn without dying first. This "becoming" is how they explain alchemy. Its process can also be expressed by the traditional formulas of initiation: the suffering‚ death‚ and resurrection of the god or the neophyte‚ represented by the substances in the crucible or by the material of the craftsman

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    LAB

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    Introduction Chemical kinetics is the study of reaction rates. A reaction rate is the speed of the change in either reactants or products over a period of time. General kinetic rate equation is: Where [A] and [B] are the concentration of the species in the reaction. The variable k is the rate constant‚ which is a function of time and catalyst presence. The variables m and n are the order of reaction for their respective species concentration. The higher the value of the reaction order the

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    Maggot Lab Mystery Lab

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    In this lab we are going to be observing the decomposition of piglets over a month’s time. There are theory questions that have been given to us before and after the lab. We look back at our original theory to see where we went wrong‚ and then correct it. The lab was disgusting‚ surprising‚ and very interesting. The first questioned to be answered is which piglet decomposes faster‚ a piglet that is in its natural state‚ that is burnt‚ that is buried‚ and that is buried in a wooden box? With

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    Lab Report Enzyme Lab

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    Abstract: The Enzyme Lab results where when the liver was frozen‚ its reaction was fast‚ and when it was hot‚ it was slow‚ and the liver that was at room temperature reacted slowly to medium. Introduction: The Enzyme Lab is to conduct investigations to determine the most favorable conditions for the most efficient enzyme activity. Variables to be used testing include temperature‚ pH values and surface area. Enzymes are proteins that speed up the rate of chemical reactions‚ which would otherwise

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    plasmid gets cut by the restriction enzyme which is an enzyme that cuts a DNA molecule. Recombinant DNA is the DNA being inserted into the E.Coli. The purpose of the lab is to see if we can make the E.Coli glow and resistant to ampicillin. In the lab we were transforming a north american jellyfish by the name Aequorea victoria to produce GFP‚ which is a fluorescent protein which causes them to glow green only if it has its friend arabinose sugar “ARA” and if it’s under an ultraviolet light . We are expecting

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    aspirin lab

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    Introduction Table of Contents Introduction Materials Chemicals Equipment Safety Containers Measuring Devices Other Equipment Procedure Synthesis of Aspirin Crystalizing the Aspirin Recrystallizing the Crude Aspirin Finding the Melting Point Range Safety Precautions Acetic Anhydride Sulphuric and Salicylic Acid Heating Observations Mass of Aspirin Synthesized Melting Point Calculations Percentage Yield Maximum Yield Crude Product Final Product Melting Range Percentage

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    Isolation and Purification of Lyngbya majuscula on Nutrient-enriched Agar Plates A Special Problem Isolation and Purification of Lyngbya majuscula on Nutrient-enriched Agar Plates ABSTRACT Lyngbya majuscula samples were obtained from the Phycology Laboratory stock culture of the UPV Institute of Aquaculture. Five (5) mm fragments of these were inoculated into agar plates that used 1% agar concentration enriched with varying concentrations (1.0%‚ 1.5%‚ and 2.0%) of Hughes‚ et

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    Lab Report Chemistry Lab

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    Since the Grignard reagent can easily react with water‚ all glassware including the 25 ml round bottom flask‚ magnetic stir bar‚ 3 and 5 ml conical vial‚ 50 mL Erlenmeyer flask‚ claisen adapter‚ drying tube and 5 glass pasteur pipets were first added to a 250mL beaker and placed in the oven for 30 minutes.  After the completion of the thirty minutes‚ 0.150 g of shiny magnesium turnings and a stir bar was first added to the round bottom flask and the claisen adapter along with the drying tube packed

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    repressor to an activator; in this experiment the pGLO plasmid has been designed with a modified operon so that in the presence of the arabinose the bacterial cells which have been transformed by the pGLO plasmid will fluoresce due to the production of GFP. SDS-PAGE is a standard technique for determining the abundance and molecular weight of a protein using an anionic detergent (sodium dodecyl sulfate or SDS) which gives the molecules a net negative charge. The charged molecules are pipetted into a gel

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