PhysioEx™ Lab: Cell Transport Mechanisms and Permeability Activity 1: Simple Diffusion Chart 1 Dialysis Results (Average Diffusion rate in mM/min) Solute Membrane (MWCO) 20 50 100 200 NaCl (—) 0.0150 0.0150 0.0150 Urea (—) (—) 0.0094 0.0094 Albumin (—) (—) (—) (—) Glucose (—) (—) (—) 0.0040 1) Which solute(s) were able to diffuse into the right beaker from the left? NaCl‚ Urea‚ Glucose 2) Which solute(s) did not diffuse? Albumin 3) If the solution in the left beaker
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Once the second 100 mL beaker reached 80°C and this was determined based on the glass thermometer. This heated solution was then poured immediately into the vacuum filtration system. The vacuum filtration system was set-up and ran identical to the experiment listed above. 10 mL of the 80°C saturated calcium hydroxide solution was measured using a 10 mL graduated cylinder. The 10 mL of the 80°C saturated calcium hydroxide solution was
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REVIEW SHEET Lab Report – Lab 3 Addendum – Cell Anatomy and Physioligy PhysioEx Worksheet Mark R. Graham 1455 Betty Court‚ Orange Park FL 32073 BSC2085C - Anatomy and Physiology I - 333738 Fall Term 2010 Larry Chad Winter lwinter@fscj.edu Submitted - 9/18/2010 NAME Mark Graham Cell Transport Mechanisms and Permeability Activity 1: Simulating Dialysis (Simple Diffusion) Chart 1 – Dialysis Results |Membrane (MCWO)
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minutes‚ then add dropwise a solution of 0.63 g sodium acetate in 2.5 mL distilled water. Heat the resulting mixture on a hot plate to between 60 and 70oC for 15 minutes‚ with stirring‚ then cool to room temperature. Isolate the product by suction filtration and wash it with 6.0 mL of cold distilled water. Suction dry for 15 minutes‚ then dry in air. Determine % yield. Recrystallize about 0.2 g of the crude
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Simple Diffusion 1. The following refer to Activity 1: Simulating Dialysis (Simple Diffusion). Which solute(s) were able to pass through the 20 MWCO membrane? According to your results‚ which solute had the highest molecular weight? ______________________________________ Which solute displayed the highest rate of diffusion through the 200 MWCO membrane? __________________________ Using the data from Chart 1‚ explain the relationship between the rate of diffusion and the size of the solute.
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8. Set up gravity filtration system using a short stem funnel (glass) lined with folded filter paper‚ covered with a watch glass and placed on top of the 125 mL Erlenmeyer flask –the flask should have a few milliliters of water so it will remain warm while using the hotplate. It is vital that the solution remain hot during filtration. If the temperature drops‚ crystals will form. Before filtration moisten the filter paper with small amount of boiling water and after filtration rinse filter paper
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the cell and the entire body. To further understand these mechanisms‚ which can be further described as passive and active transport‚ five experiments were conducted. These tests were done over simple diffusion‚ facilitated diffusion‚ osmosis‚ filtration‚ and active transport by changing and observing different variables and how they affect transport through the membrane. Obtained was the understanding of the changing of the molecular weight cut off in a membrane‚ and how it does not play a part
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Simple Diffusion Activity 1: Simulating Simple diffusion 1. What is the molecular weight of Na+? 22.99 or 23 2. What is the molecular weight of Cl-? 35.45 3. Which MWCO dialysis membranes allowed both of these ions through?50‚100‚ 200 4. Which materials diffused from the left beaker to the right beaker? Urea‚ NaCl and glucose diffused 5. Which did not? Why? Albumin was too large to diffuse into the right beaker. Activity 2: Simulating Dialysis 6. What happens to the urea concentration
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FOUR There is a direct relationship between the MWCO and the filtration rate; the higher the MWCO‚ the higher the filtration rate. Powdered charcoal is the solute that did not appear in the filtrate when using any MWCO membranes. As pressure increases‚ the filtration rate also increases‚ as long as the MWCO does not decrease. Since we have no control over the MWCO of a living membrane‚ we can increase the rate of filtration by increasing the pressure. The molecular weight of glucose
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giants consisting of a portfolio of 60 business units. In 2006 the total corporate revenue was $11 billion. One of its units is the filtration units which develop next generation of products and technologies. But after two high profile new product failure‚ the unit had lost confidence. By 2006‚ it was losing $6 million annually. Peter Vyas‚ manager of filtration unit has to decide whether to support or reject the request for $2 million in funding for RIMOS (residential irrigation mini-oxidation
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