product was formed‚ and a bubble in the Durham tube indicates that a gas was formed. In this study‚ carbohydrate fermentation tests for the presence of glucose‚ maltose‚ lactose‚ and sucrose in Proteus Vulgaris were performed. The results were then compared to the results of prior experiments. Materials and Methods: Four tubed broths (Glucose‚ Maltose‚ Lactose‚ and Sucrose) are obtained. The tubes were inoculated with Proteus Vulgaris‚ and were incubated for 24 hours at 35°C. After incubation
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experiment was to investigate the effect of different amounts of a substrate on the respiration rate of yeast and to compare this to the effect of different amounts of glucose on the rate of yeast respiration. The substrate which I chose to further investigate was fructose. Fructose is a fruit sugar which is one of the three‚ along with glucose and galactose‚ dietary monosaccharides that are directly absorbed into the bloodstream during digestion. Materials: 2% yeast solution Large beaker Small
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Identifying Biological Molecules with Colorimetric Chemical Tests Objectives Perform a series of accurate tests on biological molecules to detect the presence of carbohydrates and proteins‚ as well as the action of an enzyme on specific molecules. Introduction Unknown biological molecules can be correctly identified using various tests‚ including colorimetric chemical tests. Colorimetric tests are when a chemical reagent‚ such as Benedict’s‚ is used on a substance or solution and a certain
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The benedicts reagent test was designed to expose the presence of reducing sugars. I stated before that many of the substances I tested changed colors‚ the color being an indication of the amount in each substance. Distilled water‚ diet soda‚ and glucose‚ all resulted in blue solutions‚ which is what we started with‚ this meant that these substances did not contain reducing sugars. Sucrose changed slightly‚ indicating that‚ while very low‚ there was some presence of reducing sugars. Pine apple juice
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it in the boiling water bath for 8 minutes. Do the same for semi-skimmed milk and skimmed milk. 3. Once all 3 of the test tubes are left to cool in the air‚ observe the colours. It will be a good idea to set up a range of colour standards from glucose concentrations of 1%‚ 2%‚ 3%‚ 4% and 5% so that you can relate the colours observed to these concentrations. 4. A positive result would be from green to yellow to brick-red colour. Method (testing for non-reducing sugars) 5. Make up the
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mainly in the mitochondria and the reactants in this process are oxygen and glucose and the main product in this process is ATP as well as waste products which include carbon dioxide and water. Almost all organisms perform cellular respiration. There are two types of cellular respiration
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Lactase is an enzyme that splits up the disaccharide lactose into monosaccharides glucose and galactose‚ but its function diminishes with age resulting in a wide use of lactase supplements. This enzyme functions under specific conditions‚ so we investigated the effect different salt concentrations have on the enzyme activity. Serial dilutions were performed to prepare a lactase solution as well as twenty percent‚ fifteen percent‚ and five percent concentrations of NaCl. Three different treatments
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Microbiology Enzymology and Catalytic Metabolism Hereditary Fructose Intolerance (HFI) Hereditary Fructose Intolerance (HFI) is a genetic condition people are born with‚ usually without previous family history. Individuals with this condition have difficulty metabolizing fructose and/or foods containing fructose. The individuals liver and kidneys attempt to use this sugar for energy and due to the incomplete breakdown of fructose‚ toxic byproducts are produced which eventually leads to serious
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Investigating the effect of different concentrations of glucose on the percent change in mass of Gummy bears according to their respective colours In this experiment osmosis is the main process taking place. Osmosis is the diffusion of water molecules of a solvent which pass through a semi permeable layer and in most cases are due to a concentration gradient meaning that the water molecules travel from an area of low concentration to one of higher concentration. In this investigation‚ gummy bears
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Glycolysis Regulation Task 3 (D4) Glycolysis is the breakdown of glucose into a compound called pyruvate. A net of two ATPs are produced as the process uses two ATPs and produces four. Glycolysis consists of ten chemical reactions; each reaction is catalysed using a different enzyme. Oxygen is not required during glycolysis so it is considered anaerobic respiration. Glycolysis must be regulated so that energy is produce only when required. During glycolysis there are three enzymes that catalyse
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