Stress and Cellular Membranes Bio Lab SBI 4U1 Due: Sept 24th‚ 2010 For: Ms. Barton By: Ameema Saeed Introduction: (See attached lab) Purpose: (See attached lab) Hypotheses: Tap water will cause no damage to the membranes. The extreme temperatures of the water cooled to 0°C‚ and heated to 94-100°C will greatly damage the membranes. Extreme heat will be more damaging than extreme cold. The more concentrated the solvent‚ the more damage it will cause to the membranes‚ therefore the solvent
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1 Experiment Membrane Transport Objectives ► Referring to energy‚ what two ways can substances enter a cell? What is active transport? What is passive transport? How is osmosis related to diffusion? How can we demonstrate active transport? How can we demonstrate Brownian movement? How can we demonstrate diffusion (2 ways)? How can we demonstrate osmosis (3 ways)? In terms of relationships between substances‚ how can we define “hypertonic”‚ “isotonic”‚ and “hypotonic”? What is the relationship
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Beer’s Law is an empirical relationship that relates the absorption of light to the properties of the material through which the light is travelling. In turn‚ absorbance is proportional to concentration and the higher the concentration‚ the higher the absorbance. This experiment incorporated Beer’s Law and is focused on determining the stress that various alcohols have on biological membranes. Using five solutions of differing alcohol concentration for each of the three alcohols; methanol‚ ethanol‚
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Transport Across The Plasma Membrane Intracellular Fluid (ICF) – The two thirds of your body fluid contained inside body cells. (Intra = within). The cytosol of the cell. Extracellular Fluid (ECF) – Fluid outside the body cells. (extra = outside). Interstitial Fluid – The ECF in tiny spaces between cells (inter = between). Plasma- the ECF in blood vessels. Lymph- The ECF in lymphatic vessels. Solute – Any material dissolved in fluid. Solvent- The fluid a Solute is dissolved in. Concentration
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Membrane Transport Christopher Gaita‚ Deija Williams‚ Elisabeth Johnston & Megan Lade University of Phoenix (Online Campus) Amy Sullivan Introduction: Membrane Transport • What is membrane transport • Types – – – – Diffusion Osmosis Active Transport Endocytosis/Exocytosis Photo Courtesy Of: http://hyperphysics.phy-astr.gsu.edu/hbase/biology/celmem.html Osmosis • Example: A semipermeable membrane bag containing a 30% sugar solution is placed in a beaker of pure water. – Diffusion or osmosis
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Lab #1: Cell Membrane Prepared for Gary V. Lawrence Biology 0983 By Zane Jeffels Partners: Lily Juno‚ Huynh‚ and Lin Yan Sun Preformed: October 11th‚ 2010 Due: October 25th‚ 2010 King Edward Campus- Rm. 3275 Vancouver Community College Abstract The purpose of this lab was to determine if hemolysis would occur‚ and how long it would take to occur to red blood cells when blood suspension is introduced to solutions prepared at different temperatures
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BioLab3 Cell Membranes Lab Report Student Name: I. Diffusion Define the following terms. Solvent Solute Solution. Diffusion Concentration gradient Dynamic equilibrium EXERCISE 1 – Factors influencing rate of diffusion Predict how molecule size and temperature will affect the outcome of this experiment. Record the data from the information in the lab. Potassium Permanganate R.T. Methylene Blue R.T. Time (min) Total Diameter
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Resting membrane potentials Definition: Large nerve fibers when not transmitting nerve signals is about 90 millivolt. That is‚ the potential inside the fiber is 90 millivolts more negative than the potential in the extracellular fluid on the outside of the fiber. The Na+-k+ pump also causes large concentration gradients for sodium and potassium across the resting nerve membrane. These gradients are the following: Sodium ( outside): 142 mEq/L Sodium ( inside): 14 mEq/L Potassium ( outside):
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Purpose: The purpose of this lab is to test the effects of osmosis on eggs in hypertonic solutions and hypotonic solutions. Hypothesis: If the corn syrup is a hypertonic solution‚ then the egg’s size will shrink. If the water is a hypotonic solution‚ then the egg will swell. Materials: Styrofoam cups (2 per group) Styrofoam cups to weigh down egg (2) Balance Paper towels Vinegar (enough to cover egg) Distilled water Corn syrup Fresh eggs (2 per group) Procedure: Day
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affect the beetroot cell membrane‚ I will keep the temperature constant throughout the experiment by using a water bath and measuring the desired temperature using a thermometer. PH: I will make sure that the pH is stable (constant) and only the temperature varies this is to be done by using buffer in every test tube so as to maintain pH balance for each beetroot sample and insure that pH does not become a variable. PH is important for maintaining the integrity of the cell membrane as integral proteins
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