TRIPLE SUGAR-IRON AGAR TEST Triple sugar-iron (TSI) agar test- designed to differentiate among the different groups or genera of the Enterobacteriaceae‚ which are all gram-negative bacilli capable of fermenting glucose with the production of acid a. Differentiation is made on the basis of differences in carbohydrate fermentation patterns and hydrogen sulfide production. To facilitate observation of carbohydrate utilization patterns- TSI agar slants contain lactose and sucrose (1%) concentrations
Premium PH Glucose Enzyme
Pathology: The study of disease Etiology: The study of the cause of a disease Pathogenesis: The development of disease Infection: Colonization of the body by pathogens Virulence - the ability to infect a host Pathogenicity - the ability to cause disease in a host Outbreak – (localized epidemic) – more cases of a particular disease than expected in a given area or among a specialized group of people over a particular period of time. Epidemic – large numbers of people over a wide geographic
Premium Epidemiology
properly as to avoid any additional growth of bacteria. (Becton‚ 2004) 2) This protocol is for samples analyzed in a doctor’s office or for a routine laboratory culture that were midstream samples for urine culture or for urinary tract infections. Negative macroscopic screened urines do not routinely require microscopic examination of urine sediment. Microscopic examination of urine sediment may be warranted when macroscopic examination is positive for blood (Hb)‚ protein (greater than a trace)‚ nitrite
Premium Clostridium difficile Urinary tract infection
Microbiology Home Assignment Student Name: Aisha Mohamed K-215810920 Lecturer Name: Dr. Faruk Sarkinfada Task 1: Describe the different classes of bacteria based on: a. Cell wall composition • :Gram negative . • Gram positive. b. Nutritional requirements : • Autotroghs:take nutrition from carbon dioxide. • Heterotrophs take the nutrition from organic carbon(carbs‚lipids‚protein) • Prototroph: take nutrition from sunlight. • Chemotrophic take nutrition from chemicals. c. Oxygen requirement
Premium Carbon dioxide Oxygen Photosynthesis
Microbiology Lecture Exam #1 Outline Chapter 1: Microbiology Introduction I. Microbiology Definition II. History of Microbiology (Christian Gram‚ Robert Koch‚ Antony van Leeuwenhoek‚ Robert Hook‚ Edward Jenner‚ Louis Pasteur‚ Elie Metchnikoff‚ Dmitri Iwanowski‚ Paul Ehrlich‚ Alexander Fleming) II. Scope of Microbiology A. Infectious Agents 1. Prions 2. Viroids 3. Viruses B. Prokaryotes
Premium Bacteria
2.1 Sanitation Methods There are Four Methods that conducted on the laboratories in order to detect the presence of microorganisms. There are Rodac Method‚ Swab Method‚ Rinse Method‚ and lastly Open Dish Method and it will be discussed in detail below. 2.1.1 Rodac Method The purpose of this Standard Operating Procedure is to describe a program that will adequately measure the efficacy of disinfection of Rooms and equipment in each laboratory‚ RODAC plates can detect the presence or absence of
Free Bacteria Agar plate Microbiology
Vocabulary words II: 1. Closed system - a region that is isolated from its surroundings by a boundary that admits no transfer of matter or energy across it 2. Undefined media - basal or complex media 3. Z-streak - is a technique used to isolate a pure strain from a single species of microorganism‚ often bacteria 4. Selective media – a growth medium or culture medium is a liquid or gel designed to support the growth of microorganisms or cells‚ or small plant 5. Differential media -are
Free DNA Bacteria Metabolism
Chapter 5 – Short answer a) Phosphorylation - the addition of phosphate to a chemical compound b) What are the 3 mechanisms of phosphorylation used by organisms? • Substrate level phosphorylation – ATP is generated when a high-energy phosphate is directly transferred from a phosphorylated compound (substrate) to ADP • Oxidative phosphorylation – electrons are transferred from a group of organic compounds to a group of electron carriers (NAD+ and FAD); occurs in inner mitochondrial
Free DNA Bacteria
Major concepts to review for Exam 1: 1.) know the elements of life – and how the reduction and oxidative of some of these drive bioenergetics 2.) Know the general time line for biology and evolution on the Earth; What major events altered evolution especially in terms of microbially driven changes 3.) Carl Woese – what did he contribute? What kind of biological molecules are best to generate phylogenetic trees‚ and why? How does this compare to enzymes? 4.) What defines a species (formal definitions)
Premium Archaea Bacteria DNA replication
REVIEW SHEET‚ EXAM 3 Chapter 6‚ Nutrition and Microbial Growth Define the four major modes of nutrition in microorganisms and distinguish among them in terms of energy and carbon sources The four major modes of nutrition are: 1. Photoautotrophs: (ex. Plants‚ some protozoa‚ & alegae) -microorganism which use carbon dioxide as a carbon source and light energy from the environment to make their own food. 2. Chemoautotrophs: -microorganism which uses carbon dioxide as a carbon source and
Premium Metabolism Cellular respiration Bacteria