isolate and identify two unknown bacteria from a mixed culture provided to us by our instructor. This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. Each test performed‚ provided us with some key information about the unknown microbes in question and how the bacteria function. Materials and Methods Over a two week period‚ eight prepared types of test media were provided to identify the assigned unknown mixed cultures. Not
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Micro Biology Lab Gram Staining Lab Report Purpose The purpose of this lab was to experiment with a staining technique called Gram Staining. Gram Stains is used to identify and classify bacteria‚ it is a differential stain that allows you to classify bacteria as either gram-positive or gram-negative. The foloowing is a breif summerary of the gram staining procedure. Apply the primary stain Apply Mordant Apply decolorizing agent Apply the secondary stain Bacteria that
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we have learned in the lab to identify two unknown organisms. PROCEDURE I was given unknown #76. I performed the streak plating method with my unknown organisms. To perform this exercise I needed a TSA plate and labeled the bottom of it with my name‚ group number and organism. I also divided the bottom into three sections. After that‚ I sterilized my inoculating loop using the bunsen burner flame. After letting the loop cool‚ I reached into my unknown test tube with my loop and grabbed the organisms
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Unknown Bacterium #22 Purpose The purpose of this experiment was to find the identity two unknown bacteria in the given test tube. Materials •Bacterial Loop •Bunsen Burner • Petri Dish with agar •Crystal Violet •Gram’s Iodine •95% Ethanol •Safrin •Glass Slides •Microscope •Unknown bacteria in test tube Procedure On the first day‚ one plate was streaked qualitatively and left it in the
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Unit 6 Lab Report 1. Objective: To find the percent composition of water in an unknown hydrate. 2. Materials: Goggles‚ apron‚ evaporating dish‚ hot plate‚ and stirring rod 3. Procedure: get the mass of a evaporating dish‚ put a sample of the hydrate onto the evaporating dish and mass the dish with hydrate. Put the dish with hydrate on the hot plate on high heat. Break up any clumps that form during the heating process. Observe any color
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pathogen in the student’s blood stream would be an enzyme immunoassay (EIA) or Immonofluorescent assay (IFA) .Specimens yielding positive or equivocal results should be tested further by using a standardized western blot assay. Specimens that are negative by a sensitive EIA or IFA do not need further testing. 5: What are the characteristics of this pathogen.(Morphology‚biochemistry‚and pathogenicity).How do the possible pathogens in the case study differ? Ans: Borrelia burgdorferi is a spirochete
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| Gram Negative Unknown | Biology 3444-006 | | Lena Wallace | 11/7/2011 | | Abstract: The purpose of this lab was to identify an unknown bacteria culture using differential tests. The identification of the unknown culture was accomplished by identifying the bacteria based on its specific metabolic characteristics and morphology. It is suggested that culture 11 is a sample of Enterobacter aerogenes. Introduction: This experiment was centered on metabolic and biochemical testing
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Identification and Diagnosis of Unknown Enteric Bacteria in an Infected Patient Using an Enteropluritube Brooke Addario 2/16/2015 BIO 488C - Section J TA – Jacob Karsten Introduction: Enteric bacteria are members of the Enterobacteriaceae family of microorganisms. These gram negative‚ rod shaped‚ facultative anaerobes are found in the intestinal tracts of humans and animals. Enteric bacteria can be pathogenic; however‚ they are also a normal part of human bacterial flora and are therefore
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to develop and adjust to altering environments. That is why it is so significant to be competent to identify microorganisms. This study was conducted by applying techniques and experiments cultured in microbiology lab for the identification of the unknown microorganism. Introduction: Identification of bacteria is significant because they are not only beneficial but potentially hazardous as well. The identification of bacteria can lead to breakthroughs in healthcare regarding treatment of old and new
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GRAM STAINING EXPERIMENT CONDUCTED ON 9/29/2013 Introduction: The Gram stain is a useful stain for identifying and classifying bacteria. The Gram stain is a differential stain that allows you to classify bacteria as either gram positive or gram negative. This gram stain technique was discovered by Hans Christina Gram in 1884. The gram stain procedure separates all bacteria into one of two groups - into gram-negative bacteria which do not stain purple and into gram-positive
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