I. Title Identification of an Unknown Plasmid In this experiment‚ we determined the phenotypic capability of an unknown plasmid along with its size. With the use of gel electrophoresis‚ we analyzed the gel photograph by using a standard DNA marker‚ Lambda HindIII‚ and came to a conclusion based on our results. II. Abstract Two experiments were done to identify an unknown plasmid. The success of these experiments came from the use of modern day technology involving gel electrophoresis
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purpose of the unknown bacteria lab assignment was to select an unknown bacteria culture and‚ through a series of metabolic tests‚ identify which bacteria genus resided in the pure culture received. A nutrient broth inoculated with bacterial culture (numbered 45‚ henceforth referenced as U45) was selected and a streak plate was made to isolate a pure culture for use throughout the assignment. From the streak plate‚ several slides were made to determine the morphology of unknown 45. A Gram stain‚ used
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we have learned in the lab to identify two unknown organisms. PROCEDURE I was given unknown #76. I performed the streak plating method with my unknown organisms. To perform this exercise I needed a TSA plate and labeled the bottom of it with my name‚ group number and organism. I also divided the bottom into three sections. After that‚ I sterilized my inoculating loop using the bunsen burner flame. After letting the loop cool‚ I reached into my unknown test tube with my loop and grabbed the organisms
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The purpose of Module 11A was to test for the presence or absence of a particular set of functional groups through the use of wet chemical tests. In this manner‚ Unknown A which was a colorless solution‚ was first tested with 2‚4-DNP which after mixing for a few seconds formed a bright yellow precipitate. Although this confirms the presence of either a ketone or aldehyde group‚ one simple chemical test does not completely specify the presence or absence of other functional groups. Therefore‚ a second
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EXPERIMENT 1 IDENTIFICATION OF UNKNOWN COMPOUNDS Date of experiment : 7th August 2012 Venue: ME204 INTRODUCTION Chemical reactions are regularly categorized into 3 types: oxidation-reduction (redox reaction)‚ precipitation (double displacement) and acid-base reaction (double displacement) Type 1: Oxidation-Reduction Reactions Oxidation-reduction processes include the movements of electrons form oxidants to reductants‚ which lead to increases in oxidation
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Unknown Bacteria Lab Report Introduction The purpose to this lab was to identify an unknown bacteria from a mixed culture provided to us by our instructor. This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. Each test performed‚ provided us with some key information about the unknown organism in question and how the bacteria function. Over a two week period‚ eight prepared types of test media were provided to identify
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It Smells Like Proteus vulgaris Microbiology Lab Report for Unknown Robert Bhowanidin MCB 2010L / Section 1290 October 24th‚ 2013 The following report will describe both my journey to find my unknown as well as the results that led to my discovery. Before I start‚ I will say that I am 100% positive that my unknown (which was #31) is none other than Proteus vulgaris. My data and the ensuing results from them simply cannot be disputed. From my first batch of results‚ Proteus vulgaris reared
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Unknown Bacterium #22 Purpose The purpose of this experiment was to find the identity two unknown bacteria in the given test tube. Materials •Bacterial Loop •Bunsen Burner • Petri Dish with agar •Crystal Violet •Gram’s Iodine •95% Ethanol •Safrin •Glass Slides •Microscope •Unknown bacteria in test tube Procedure On the first day‚ one plate was streaked qualitatively and left it in the
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The conventional method was conducted by placing 2.0641 grams of salicylic acid in a dry 125 mL Erlenmeyer flask. In a fume hood‚ 4 mL of acetic anhydride and 5 drops of concentrated sulfuric acid were added to the flask. The contents were swirled in the flask and then heated for 20 minutes in a hot water bath. After the 20 minutes were up‚ 10 mL of cold water were added in 1 mL increments. The flask was cooled for 10 minutes and occasionally stirred. The crystals that formed were collected via filtration
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A Critical Analysis of ‘The Tale of the Unknown Island’ by José Saramago. | EN4903 | By Mr Drew Eaglesham | A Critical Analysis of ‘The Tale of the Unknown Island’ by José Saramago. ------------------------------------------------- ------------------------------------------------- Kirkus in 2010 called this story ‘a mischievous and thoughtful satire on ruling elites and bold dreamers‚ cast in the form of revisionist fairy-tale.’ This review could easily be applied to another story of Saramago’s
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