Gram staining From Wikipedia‚ the free encyclopedia A Gram stain of mixed Staphylococcus aureus (Staphylococcus aureus ATCC 25923‚ Gram-positive cocci‚ in purple) andEscherichia coli (Escherichia coli ATCC 11775‚ Gram-negative bacilli‚ in red)‚ the most common Gram stain reference bacteria Gram staining (or Gram’s method) is a method of differentiating bacterial species into two large groups (Gram-positive and Gram-negative). The name comes from its inventor‚ Hans Christian Gram. Gram staining
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Microbiology Unknown 5/7/2013 Microbiology Unknown #94 The purpose of this report was to identify an unknown bacterium by applying methods that were previously learned and conducted in microbiology lab class. It is possible to identify a mixed culture by running experiments on the unknown bacteria and taking what we already know and applying it to the results. It helps to slowly eliminate any bacteria that do not correspond with the results of certain tests. An unknown test tube labeled 94 was
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staining; B. enumerate the common stains; C. differentiate between an acidic dye and a basic dye; D. compare simple‚ differential and special stains; and E. list the steps in preparing a Gram stain and describe the appearance of a gram-positive and gram- negative cells after each step.\ II. SUBJECT MATTER: Biological Techniques Topic: Staining A. Materials: * cartolina- used during the activity in lesson proper(flashcards) * iodine-
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Biology 205 MW 2:10pm. Unknown Organism Paper The unknown organism tested in the Mesa lab was collected from in between the toes of my puppy‚ Riley. The original culture grown consisted of dozens of visibly different organisms of varying colony colors and growth patterns. I chose to test a smaller‚ red colony for my experiments. Based on its gram reaction and oxygen requirements‚ the following tests were performed to reach my presumptive ID: Test Performed Result Gram Stain + Fluid Thioglycollate
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Identification of Unknown # 15 Abstract. One of the most fundamental differential staining techniques used in the study of bacteriology is gram staining. There are two main types of bacteria‚ gram negative and gram-positive. The purpose of this experiment was to perform a variety of tests to identify the bacteria contained in the unknown sample labeled number 15. The following are the tests that were used to identify the two different bacteria. The
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GRAM STAIN THEORY: The most widely used staining procedure in microbiology is the Gram stain‚ discovered by the Danish scientist and physician Hans Christian Joachim Gram in 1884. Gram staining is a differential staining technique that differentiates bacteria into two groups: gram-positives and gram-negatives. The procedure is based on the ability of microorganisms to retain color of the stains used during the gram
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Micro Biology Lab Gram Staining Lab Report Purpose The purpose of this lab was to experiment with a staining technique called Gram Staining. Gram Stains is used to identify and classify bacteria‚ it is a differential stain that allows you to classify bacteria as either gram-positive or gram-negative. The foloowing is a breif summerary of the gram staining procedure. Apply the primary stain Apply Mordant Apply decolorizing agent Apply the secondary stain Bacteria that
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Identification of Two Unknown Species of Bacteria in Tube #72 Introduction: There are many reasons for knowing the identity of microorganisms. The reasons range from knowing the causative agent of a disease in a patient‚ so as to know how it can be treated‚ to knowing the correct microorganism to be used for making certain foods or antibiotics. This study was done by applying all of the methods that have been learned so far in the microbiology laboratory class for the identification of an unknown bacterium
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on human samples collected from different body sites. The tests are used to detect and identify any microorganisms capable of causing disease. Knowing of unknown microorganism is important on how this microorganism works and how it is structured‚ means knowing how it can affect humans. The purpose of this study was to identify an unknown bacterium by applying all methods that were previously conducted and learned in the medical microbiology laboratory class. **Materials
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The Gram staining method is named after the Danish bacteriologist who originally developed it in 1882‚ Hans Christian Gram. The Gram staining process is one of the most important staining techniques in microbiology. It is almost always the first test performed for the identification of bacteria. The primary stain of the Gram’s method is either crystal violet or methylene blue‚ each almost equally effective. The microorganisms that retain the crystal violet and iodine mixture appear purplish brown
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