Bacterial Transformation Lab Report Backround: The plasmid pGLO contains an antibiotic-resistance gene‚ ampR‚ and the GFP gene is regulated by the control region of the ara operon. Ampicillin is an antibiotic that kills E. coli‚ so if E. coli‚ so if E. coli cells contain the ampicillin-resistance gene‚ the cells can survive exposure to ampicillin since the ampicillin-resistance gene encodes an enzyme that inactivates the antibiotic. Thus‚ transformed E. coli cells containing ampicillin-resistance
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in tea‚ coffee‚ cocoa‚ OTC medication‚ and cola drinks.It stimulates the brain and the central nervous system‚ so that a person feels less tired and more alert. The purpose of this lab is to determine the amount of caffeine in tea. The major component of tea is cellulose which is a polymer of glucose. Materials Lab coat Goggles Graduated cylinder 1 tea bag (1.615g) 50 mL beaker Ice Gloves Hot plate 500 mL Erlenmeyer flask 30 mL DI water Wash glass Ca2CO3 DCM Funnel Stopper
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Formal Scientific Lab Report Osmosis Katy Hunter 10-26-2012 Abstract: The objectives of this lab was to be able to create models of cells with the dialysis tubing to show us how the plasma membrane is selectively permeable‚ to study the effects of osmosis on a model cell‚ and to foresee the effect of solute concentration on osmosis. In order to achieve these objectives‚ we had to fill the dialysis tubing with either water‚ or different amounts of sucrose. We then tied off the tubes and put
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Those colonies were subjected to a Gram-stain to ensure that P. larvae‚ a Gram-positive bacterium was isolated (Figure 7). However‚ there was a presence of other bacteria visible on the Gram-stain. Therefore‚ the catalase negative colonies were streaked onto MYPGP agar to further isolate P. larvae. The pale colonies that grew on those plates were subjected to a
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A. Goal and Purpose: Session 1: In this lab‚ we will achieve a simple Friedel-Crafts alkylation of anthracene. The choice of anthracene as an aromatic substrate stems from two considerations. First‚ there is a question of regioselectivity. Second‚ anthracene and its derivatives are highly visible under UV light. Session 2: In this lab‚ we will complete a partial conversion of 9-acetylanthracene using m-chloroperoxybenzoic acid (mCPBA). We will also determine by NMR‚ the regiochemistry of the
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SCIE211 Phase 2 Lab Report Title: Speciation Instructions: You will need to write a 1-page lab report using the scientific method to answer the following question: • What would happen if a species within a population were suddenly split into 2 groups by an earthquake that creates a physical barrier like a canyon? When your lab report is complete‚ post it in Submitted Assignment files. Part I: Use the animated time progression of speciation to help you write up your lab report. Part II: Write
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GAUTENG DEPARTMENT OF EDUCATION SENIOR SECONDARY INTERVENTION PROGRAMME English FAL Grade 12 SESSION 15 (LEARNER NOTES) LITERATURE: STUDYING DRAMA Learner Note: It is difficult to discuss a specific DRAMA in this session‚ since all schools are not doing the same prescribed work. This session will focus more on introductory notes on the study of drama and will include extracts from some of the prescribed plays. It is‚ however‚ very important that you know the basics of studying drama – once you
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I had # 8 organism‚ Bacillus subtilis. Bacillus subtilis is a Gram-positive bacteria‚ rod-shaped and catalase positive. In order to figure out what is my unknown organism for this assignment‚ I had to perform series of tests. First was the glucose‚ lactose‚ and sucrose test. Through these three tests‚ I was able to detect the ability of the microorganism to ferment a specific carbohydrate. Fermentation reactions are observed by the color change in pH. In class‚ we used phenol red as the pH indicator
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allow an object to move is dissipated into heat energy and will not return to the system once the movement stops. Specifically‚ this lab will calculate the coefficient of friction. Unlike most coefficients in Physics‚ friction behaves differently depending on whether the object is at rest or at motion.
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Our main goal was to test organisms’ resistance and susceptibility to bacteria‚ and whether the cell wall of bacteria exposed the membrane to pathogen or protect it from pathogens. I thought P. aeruginosa was gram-positive and therefore it would be susceptible. That was my hypothesis.We divided antiseptic agar plate into quadrants and divided antibiotics agar plate into six areas. We wrote the organisms’ name‚ date‚ and the temperature on the bottom of the agar plates. For antiseptic‚ our group used
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