Gram Staining Gram Staining is a method of differentiating bacterial species into two large groups (Gram-positive and Gram-negative). Gram Staining is a way to separate one large group of bacteria into two. Crystal violet is used to dye the cells‚ which is the primary stain. Those that retain the color are grouped as Gram-positive‚ and those that do not retain the color are grouped as Gram-negative. Many of the Gram-negative bacteria are pathogenic‚ making this process useful for detecting infections
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future use in various fields. -Simple stain helps with determining the sizes‚ shapes and arrangements of microorganisms. -Gram stain assists in identifying the type of microbes‚ whether they are gram positive or negative‚ which in turn tells us what kind of cell wall the microbes have. -Endospore stain tells whether microorganisms make endospores. -Acid-fast stain is helpful in recognizing the existence of mycolic acid in cell walls. -Capsule stain reveals the existence of capsules.
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Medical Mycology: Yeast and Pneumocystis| Reading Assignment:|Mahon‚ Chapter 10‚ pgs 215-219‚ Chapter 27‚ pgs 626-629‚ 634-636‚ Appendix B Lecture Notes: Medical Mycology| |U of W Tutorial on Mycology (organisms listed in objectives)‚ www.medtraining.org[->0]| _____________________________________________________________________ 1. Discuss the difference between yeasts and molds. Fungi seen in the clinical laboratory can be generally separated into two groups based on the appearance of the
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There can be intentional stains (such as wood stains or paint)‚ indicative stains (like food coloring or adding a substance to make bacteria visible under a microscope)‚ natural stains (such as rust on iron or a patina on bronze)‚ and accidental stains (like spilling ketchup on your shirt.) While the types of stains are very different in application‚ they all form in the same basic ways: The primary method of stain formation is surface stains‚ where the staining substance is spilled out onto the
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Identification of Unknown # 15 Abstract. One of the most fundamental differential staining techniques used in the study of bacteriology is gram staining. There are two main types of bacteria‚ gram negative and gram-positive. The purpose of this experiment was to perform a variety of tests to identify the bacteria contained in the unknown sample labeled number 15. The following are the tests that were used to identify the two different bacteria. The
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Name SOLUBILITY CURVES Answer the following questions based on the solubility curve below. Which salt is least soluble in water .. at 2O° C? 2. How many grams of potassium chloride can be dissolved in 200 g of water at 80° C? IO 3. At 40° C‚ how much potassium _ __nitrate coin be dissoiu$tl ^n 30D.g of water? ------W- ’1 80 70 ...- O --60 0 5© 40 4. Which salt shows the least change 30 In solubility from 0° - 100° C? 20 10 At 30° C‚ 90 g of sodium
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difference between a gram positive bacteria and gram negative bacteria is the differences in cell wall composition. Prokaryotes known as eubacteria have three basic forms: rods‚ cocci and spiral. The bacterial cell wall is the single most important contributor to cell shape. In addition to shape of cell wall‚ presence or absence of flagellum‚ and if present‚ positions of flagellum‚ the eubacteria can be classified according to Gram Stain. First and foremost‚ gram positive bacteria are
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An Essay On Poop Stains Issues surrounding poop stains can never be over analyzed. I find my self constantly drawn back to the subject of poop stains. While it is becoming a hot topic for debate‚ it is important to remember that ‘what goes up must come down.’ Since it was first compared to antidisestablishmentarianism much has been said concerning poop stains by so called ’babies’‚ whom I can say no more about due to legal restrictions. With the primary aim of demonstrating my considerable intellect
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Cucumber as stain remover For kitchen sinks A research report Submitted to zamboanga Del Sur National High school Special science curriculum as an entry for the science fair S.Y 2013-2014 Researchers: 1. Dia-una‚ Rachell 2. Lagang‚ Ella Mae 3. Villarin‚ Mitzi 4. Dumanjug‚ Hassan 5. Balimbingan‚ Muhader 6. Canencia‚ Rosevick 7. Manupac‚ Aldemmer 8.
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juice stain on six cotton tee shirt cut outs. I used scissors to cut small pieces of fabric in to square shapes. I researched and found that cotton holds stains very well. To remove each stains from the fabric I used baking soda‚ ketchup‚ Gatorade‚ lemon juice‚ detergent‚ and just water. The investigation I conducted was‚ “What household items can you use to remove a stain the best.” For my procedure‚ I cut out six pieces of a cotton tee-shirt and applied one tablespoon of a grape juice stain. One
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