Sarcina lutea‚ Pseudomonas fragi‚ Micrococcus luteus‚ Alcaligenes faecalis‚ Clostridium sporogenes‚ and Micrococcus roseus. There were several qualitative tests that could be conducted to determine the identity of the unknown species‚ for example‚ Gram staining‚ Fermentation‚ Catalase‚ Oxidase‚ Starch Hydrolysis‚ Litmus Milk‚ MOI medium‚ and the Gelatin Test. All tests and techniques used were performed in accordance with The Microbiology Lab Manual. Materials and Method: A stock broth culture
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colored colony. Gram-staining was utilized as a first step to differentiate between gram-positive and gram-negative. After determining that it was a Gram negative single bacillus‚ biochemical tests performed and their purpose and results are shown in table and flow chart form. Table 1: biochemical Test Results Test | Purpose | Reagents | Observations | Results | Gram stain | To determine the Gram reaction of the bacterium | Crystal violet‚ Iodine‚ Alcohol‚ Sarasin | Pink rod | Gram-negative rods
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Since it was gram positive‚ I knew my organism had a thick peptidoglycan and no outer membrane. After finding out my organism B was gram positive‚ I did the catalase test on it. After adding the two drops of H202 and seeing bubbles‚ I ruled out Streptococcus lactis. My organism did have the catalase enzyme; meaning it was
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as a Counterstain in Gram Staining Investigators: Astilla‚ Yasmin Marie Cala‚ Maria Victoria Bianca Ko‚ Mariell Lizardo‚ Francis Dominic Mariano‚ Maria Cristina Querol‚ Maria Zennah Quinto‚ Camille Rosales‚ Ana Mariella Valmonte‚ Kyla Nicole Villalon‚ Gian Angelo 4MT-2 Statement of the Problem Can Basella rubra fruit extract be used as a counterstain in Gram staining? Introduction & Background According to H. J. Croxx‚ when microscopists first began to use stains in the sixties and seventies
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included: Gram stain‚ mannitol salt agar‚ coagulase tube test‚ and an antimicrobial susceptibility test. The tests selected were based on the results of a gram stain. Gram staining‚ the most commonly used differential stain‚ allows for the fast and easy detection between gram negative and gram positive organisms based on the presence or absence of peptidoglycan. A gram positive organism resists the decolorization process due to a large amount of peptidoglycan and appears a purple color. A gram negative
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the plate‚ it could be seen that the unknown had growth and formation of colonies. The Gram stain was chosen next to determine if the unknown was gram-negative or gram-positive. After looking at the gram stain under the microscope with the oil immersion it could be determined that the unknown was gram-negative due to the fact that the bacteria was pink in color and rod shaped. After determining the unknown was gram-negative‚ other bacteria’s were able to be eliminated from the options. The Triple Sugar
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different cultures were observed. In order to proceed identification‚ those cultures were isolated as separate organisms‚ using inoculating streak technique and incubated until the next class period. The expected result was supposed to show Gram positive and Gram negative bacterium growing on a separate media. Having checked two Nutrient plates‚ signs of visible growth of two different bacterium were present. Due to the fact‚ that one type of bacteria didn’t appear to be fully
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biotechnicalfieldsThe cell wall is the basis for classification of bacteria according to the Gram stain. Accordingto the chemical make up of bacterial cell wall‚ a staining procedure‚ Gram stain‚ helps usclassify bacteria into two subgroups‚ The cell wall can stain either positive or negative‚depending on its chemistry. If the bacteria stains positive it will retain a purple/blue color. If the bacteria stains negative‚ the bacteria will not retain the purple/blue color‚ but rather have a pinkish/red
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test the purity of the unknown. After the plates were incubated and grown‚ the morphology was observed and recorded and a Gram stain was performed. After determining the Gram reaction‚ specific biochemical tests were performed. The biochemical tests were chosen from the unknown identification tables that were in the lab manual. Since the unknown #54 was determined to be a Gram positive rod‚ a thyoglycollate test was performed to determine aerobic activity. Table 1 lists the test‚ purpose‚ reagents
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What is a differential stain? How is it different from a simple stain? 1. A differential stain is a stain that differentiates between gram-positive and gram-negative bacteria. Bacteria that stain purple are considered gram-positive and those that stain pink are considered gram-negative. 2. A simple stain is a stain that reacts with all microbes in and identical fashion. They are used to increase contrast. 3. They are different from one another because Differential stains affect specific microbes
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