conducting a Gram stain on my bacteria. I prepared a smear by placing a drop of water onto the center of a slide and then removed a loop full of bacteria to place within the water droplet. I use the aseptic treatment while doing this making sure to flame the inoculating loop before and after transferring bacteria. It was important to get this done correctly because the test determines the stain as well as the shape of the cells. After looking at my stain under the microscopic I came to the conclusion that
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Which of the following stains is used frequently to identify Mycobacterium and other bacteria whose cell walls contain high amounts of lipids? A. Gram stain B. Schaeffer-Fulton stain C. Acid-fast stain D. Lipidialar stain E. Spore Stain 2. Which of the following stains is used to classify microorganisms based on their cell wall content? A. Capsular stain B. Gram stain C. Spore stain D. Negative stain E. Methylene blue 3. In the Gram stain‚ Gram-positive bacteria stain with a _________ color due
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Microbiology 5/14/2009 Introduction The purpose of this study is to differentiate and identify two unknown organisms provided by the instructor in a nutrient broth. It is only known that the two organisms are from vomit; one is gram-positive and the other is gram-negative. It is necessary to first separate the two organisms by inoculating a nutrient agar plate using the streak-plate method. The initial streak-plate procedure was performed and placed in the incubator at 37◦C for 24-48 hours
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survivability in artificial gastric juice ABSTRACT: Two probiotic drinks were investigated in vitro to test their ability to survive acidic conditions and their probiotic factors. Both the products: Actimel and Yakult contain gram-positive bacteria‚ but Actimel also has a gram-negative bacteria. The ability to survive was investigated by adding artificial gastric juice to the products and incubating at different times. Actimel and Yakult were both able to survive the gastric juice. Actimel produced
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number 8 was given out by the lab instructor. The goal at this point was to determine unknown gram positive vacteria. The procedures performed consisted of sterile technique in addition to being followed as stated in the referenced course laboratory manual by Matar (1) ‚ unless otherwise noted. Not all of the tests were performed on every culture. However‚ there are as some of the tests were used only for gram (+) others were even more specific and used only for cocci bacteria .The first procedure have
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Questions A. What is a differential stain? How is it different from a simple stain? the use of two or more stains to categorize cells into groups. simple stain uses one dye and shows only structure‚ morphology and size. a differential stain uses two or more dyes and is used to show morphology and distinguish cell types B. What is the difference between Gram-positive and Gram-negative cell walls? Structure: Have thick layer of peptidoglycan
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battery of tube tests and plates designed to lead to identification of two unknown bacterial species‚ from the combination of all results. A sample of bacteria was used‚ labeled “Sample 4”‚ from which both species was to be obtained‚ one gram positive and one gram negative. Table 1 is a list of the possible bacteria to be identified; the basic ideas and practice of identification of an unknown sample of bacteria are important for a microbiologist to develop. Not only is proper procedural practice
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Choice 1. Which of the following stains is used frequently to identify Mycobacterium and other bacteria whose cell walls contain high amounts of lipids? Gram stain Schaeffer-Fulton stain Acid-fast stain Lipid stain Spore Stain 2. Which of the following stains is used to classify microorganisms based on their cell wall content? Capsular stain Gram stain Spore stain Negative stain Methylene blue 3. In the Gram stain‚ Gram-positive bacteria stain with a _________ color due to the incorporation
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glass slide. Set the slide to air dry. Either heat fix or methanol fix. (do not fix it both ways) Stain the slides. Properly dispose of any wastes.(waste methanol‚ materials contaminated with bacteria‚ broken glass) Part II. Atlas Sections In your Lab Atlas you will need to read Section 5: Bacterial Cellular Morphology and Simple Stains and Section 6: Bacterial Cell Structures and Differential Stains and then answer the following questions: 2. What is the third important feature of microscopy?
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Gram Characteristics of Bacteria Introduction Bacteria are interesting little critters in that they are not easily visualized without some sort of stain. In classical methodology‚ two different kinds of stains have been used to identify bacteria: acidic and basic dyes. Acidic dyes are so called because they consist of a salt that has a cation that transfers no color‚ but the anion portion is colored and does give off color. Basic dyes consist of an anion that does not give off any color and a cation
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