microorganisms is a practice that aids in Increasing ones knowledge of a laboratory setting and it helps improve on Using sterile technique. Isolates of soil microbes can be categorized and Characterized based on a number of criteria ranging from gram-staining Which is done for this project to enumeration which is quantitative description Based on the amount of microbial colonies available. The Antimicrobial Activity of three different microbes were also tested and results were obtained
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the lid back on. Never set the lids of the tubes or plates on the table when removed for inoculation of bacteria(5). The first thing to check off the list was the gram stain and the shape of the bacteria. Next‚ the lab manual gave further specifics for each type of biochemical tests(5). Except for determining the shape and the gram stain‚ the rest of the tests are on agar plates‚ in agar tubes‚ and in broth tubes. The name of each test performed are listed below in the order the tests were done in
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accessible. Conclusions – The experiment was interesting as the first portion consisted of a virtual microscope‚ it allowed for a different magnification view and I was able to view these as often as necessary. The other three exercise were not difficult to do‚ however it was getting the supplies in order for the experiments that I found much difficulty in. Questions A. List three reasons why you might choose to stain a particular slide rather than view it as a wet-mount. Gram stains provide a more
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particular order. Name this Procedure Identify the two types of bacteria present by shape and gram stain. In a gram stain what is the primary stain? The mordant? The counterstain? How does this differ from a simple stain? Identify the shape and gram stain of the bacteria present in this gram stain. Identify the shape and gram stain of the bacteria pictured. Think on this one! Name the gram stain and shape of the bacteria pictured. Name the genus of the organism pictured. Note the size when
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Having the ability to collect and identify unknown microorganisms is vital in health and medicine. This capability is important for a variety of reasons‚ such as knowing the causative agent of disease‚ knowing if the microorganism obtains any beneficial properties and knowing the correct microorganism to use to create a successful antibiotic. Implementing the experimental methods learned thus far in the microbiology laboratory allowed an unknown bacterium to be identified as a result of this study
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to stain microorganisms was a significant advance in microbiology. Stains serve purposes such as it differentiate microorganisms from their surrounding environment and it allows detailed observation of microbial structures at high magnification. (http://inst.bact.wisc.edu/‚ © 2006-2013 Microbiology Laboratories) Gunasekaran (2005) defined staining as the method of artificially producing color in microorganisms to allow for the visualization under the microscope. He further stated that stains are
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Biology 2020 Microbiology Study Guide for first exam The history and scope of microbiology 1) What are the organisms/entities of study in the field of microbiology? * Bacteria * Archaea * Eukarya * Viruses and Prions (Acellular entities) 2) Who was the first human to publish extensive descriptions of microorganisms? * Antony van Leeuwenhoek 3) How did Louis Pasteur dismantle the theory of spontaneous generation in bacteria? * Swan neck flask experiment: Flask
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pinkish in color. The first test we did was basic stain using a heat fixed emulsion which kills the bacteria and allows them to adhere to the slide and thickens their protein for better staining. I then covered the heat fixed emulsion with crystal violet for one minute. The stain was then rinsed with distilled water. The stain was then pat dry with bibulous paper then observed under oil immersion to determine morphology. Upon completion of the basic stain organism appeared to be cocci that was in a line
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physiology‚ antibacterial susceptibility‚ selective media‚ and biochemical provide results. Both the unknown isolate and members of the Micrococcus genus were shown to be obligate aerobes. By using staining methods‚ this proved that the organism is gram positive. Morphology‚ such as‚ orange pigmentation and coccus shape provide similarities to the Micrococcus genus. Physiological tests were shown to be obligate aerobe‚ mesophile‚ neutrophile‚ and osmotolerant organism. Biochemical reactions such as
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covering it with a cover glass using no stain. It is best viewed with as little illumination as possible and must be done quickly because of drying. Due to the limited stability of Trichomonas‚ the wet mount is not a suggested test choice in an outpatient location where transportation of the specimen to the lab is involved Exercise 2: Simple stains Define acidic and basic dyes. What is the purpose of each? Acidic dye also recognized as a negative stain‚ is defined as a negatively charged dye
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