order to identify an unknown bacterium. An unknown bacterium was handed out by Dr. Honer. The appropriate tests were prepared and applied. The first procedure that was done was the gram stain. Under a microscope‚ if the gram stain is purple‚ the bacterium is gram positive‚ if the stain is red‚ it is gram negative. The next test was the fermentation tests for glucose‚ sucrose and lactose. If an organism is able to ferment the carbohydrate‚ there will be acid produced‚ which will turn the broth
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bacteria are not gram positive‚ but this lab will examine only this kind because of their influence in human health. Staphylococcus Aureus is a gram-positive coccus bacterium found in humans. It is frequently found on the mucus membrane in the nasal cavity and on the skin. It also infects many people in the hospital‚ also called a nosocomial infection. S. aureus has a large association with clinically significant infections and
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master my skills of using the Gram stain‚ testing for catalase‚ testing for coagulase and using the oxacillin screen. Now I am going to take you with me on the journey to the unknown. As I recall from earlier that semester‚ the first test that needed to be performed was the Gram stain. The Gram stain is a staining technique that aids in distinguishing cell wall characteristics and the cellular morphology. Bacteria will stain Gram positive‚ which is a purple color‚ or Gram negative‚ which is a pink
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procedure. Do not include any observations or results in this section> Observations and Results Part I: Bacterial Growth Result + or - Appearance after Incubation Gram Stain N/A OF Glucose Broth OF Glucose Broth with Oil Indole Production Test Malonate Bacterium identified as: Assigned bacterium was: Discussion & Conclusion Based upon your results‚ is your bacteria sample a strict aerobe‚ strict anaerobe‚ facultative anaerobe‚ or microaerophile?
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Plasmodium‚ Trypanosoma Algae: Diatoms‚ Spirogyra‚ Volvox Helminths: Tapeworm‚ fluke Fungi: Methylene blue stain of yeast cells (Saccharomyces)‚ sporangiospores of Rhizopus‚ conidiospores of Penicillium Staining Labs (3-6‚ 3-7‚ 3-8‚ and 3-9) – Identify and correctly interpret the following: Lab 3-6: Gram stain Lab 3-7: Acid Fast stain Lab 3-8: Capsule stain Lab 3-9: Endospore stain Metabolic Tests: Lab 5-1: O-F Glucose (aerobic versus fermentative organisms) Lab 5-2: Phenol Red broth for
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My partner and I were given unknown number 3 in the laboratory. After performing various tests over the course of a few weeks on our unknown‚ we came to the conclusion that our unknown organism is Klebsiella pneumoniae (K. pneumoniae). K. pneumoniae is a gram negative bacillus shaped microorganism. We observed that K. pneumoniae is a nonmotile organism. We performed multiple tests on our unknown culture‚ therefore we are very confident that it is correctly identified. We identified that K. pneumoniae
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Tests done Test | Purpose | Reagents | Result (Positive) | Result (Negative) | Gram Stain | 1. To determine the cell wall formation ( thick layer peptidoglycan or thin layer peptidoglycan) | Crystal violet‚ iodine‚ 95% Alcohol‚ Safranin | Purple specimen- Gram Positive‚ cell wall has thick peptidoglycan layer | Pink Specimen- Gram Negative‚ cell wall has thin peptidoglycan layer | Catalase Test | To determine the production of catalase enzyme. The enzyme
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At the beginning of the semester‚ samples of bacteria were obtained from various sources. Two bacterial colonies were isolated from plates that were incubated in the lab and these bacteria became the basis for this project. The bacterial cultures were maintained and used for various physiological‚ cultural‚ and biochemical tests and observations over several weeks. The purpose of culturing bacteria over half the course of the semester was to learn various techniques used in microbiology labs and
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for gram (+) or gram (-) microbes‚ while others aren’t specific. On the nonspecific TSA plate a large hazy white colonies was formed. On the other hand‚ there were also small orange colonies fewer in growth. Isolation onto a BAP would allow for a pure culture of just gram (+) which would be used to carry out the rest of the differential test. Isolation onto TSA/ MAC allowed for the growth of gram (-) and would be used for further test. Procedure: The catalase test was performed only on gram (+)
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my unknown identification was to carry out a gram stain to observe the cell shape and arrangement. My unknown turned out to be gram positive bacteria cocci shaped with long chain cell arrangement. The unknown was gram positive because the cells stained blue-violet ‚this indicate that my unknown has ability to retain crystal violet-iodine after alcohol application on the cells which is a gram positive characteristic caused by more peptidoglycan than gram negative organisms on cell walls . To observe
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