ASEPTIC TECHNIQUES AND CELL COUNTING INTRODUCTION:- Microbes are single celled organisms and are so small that we cannot see with our naked eyes‚ microbes can only seen under microscopes .Microbes are the one of the oldest living form on earth‚ fossils of microbes were found which are said to be 3.5 billion years old . Microbe world: what is a microbe [online].[Accessed 9 April 2013].Available from: world wide web:‚microbes are found everywhere in rocks‚air‚water some of the microbes are even
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Pseudomonas and identifying it using phenotypic properties and DNA sequencing as an existent or completely new and undiscovered species of Pseudomonas. Overview: Genus Pseudomonas is a chemoheterotrophic bacteria found in soil and water. They are Gram negative‚ motile‚ paired rods that are also oxidase-positive. Pseudomonas species are well known for their ability to metabolize many different organic nutrients‚ allowing them to be viable in multiple‚ diverse conditions. The Pseudomonas organisms
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MBK Lab 01 – Lab Report Name: ____________________ Section: ___________________ EXPERIMENT 1 TITLE: Observing Bacteria and Blood OBJECTIVE: To gain functional knowledge of microscope operations through practical applications of a microscope in the observation of bacteria and blood. PROCEDURES: Using the microscope‚ an oil immersion lens and observing Bacteria Cultures in Yogurt . Preparing a Blood Slide and observing Blood: After reviewing the section of the manual
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product can be stained with the gram staining. Gram staining is the method of differentiating bacteria into 2 large groups of Gram-positive bacteria and Gram-negative bacteria based on their cell wall. Most of the bacteria or yeast in the starter cultures used are Gram-positive. Gram staining requires 4 types of solutions used in the procedure: crystal violet‚ mordant‚ alcohol and safranin. The Gram-positive microbes will have blue stain while Gram-negative stains pink after the procedure. Methylene
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slides for gram staining and viewing under a microscope. {Either my gram-stain slides were bad or the microscopes I chose for viewing were not good. No bacteria were found under the microscope.} Prepare another gram stain of the unknown bacteria. {Gram-positive‚ coccus shaped bacteria were observed under the microscope. Also noted‚ the slide may have been inadvertently contaminated during the gram stain process by another person in the laboratory.} [I do think that the gram-stain result is
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Microbiology Lecture Exam #1 Outline Chapter 1: Microbiology Introduction I. Microbiology Definition II. History of Microbiology (Christian Gram‚ Robert Koch‚ Antony van Leeuwenhoek‚ Robert Hook‚ Edward Jenner‚ Louis Pasteur‚ Elie Metchnikoff‚ Dmitri Iwanowski‚ Paul Ehrlich‚ Alexander Fleming) II. Scope of Microbiology A. Infectious Agents 1. Prions 2. Viroids 3. Viruses B. Prokaryotes
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Identification of Bacterial Pathogens basic skills in diagnostic bacteriology Dr.T.V.Rao MD Dr.T.V.Rao MD 1 Identification of Microorganisms • For many students and professionals the most pressing topic in microbiology is how to identify unknown specimens. • Why is this important? • Labs can grow‚ isolate and identify most routinely encountered bacteria within 48 hrs of sampling. • The methods microbiologist use fall into three categories: ♣Phenotypic- morphology (micro and
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Online BIO 150 Introductory Microbiology #3 Lab Report NAME __ Lab Group 2_____ Answer the following questions as you work your way through the lab material typing in your answers. Then submit your finished lab report as a Microsoft Word document. This lab report is worth 100 points towards your final lab grade. Each Q is worth 2 points unless otherwise noted. Also‚ per the Honor Code‚ this work must be your own. This is due Mon. 10/8 at 11:59 PM. The theme of this lab is the identification
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What type of lens does a microscope use to produce a magnified image? How does a lens produce a magnified image? 3) What are some ways to increase contrast in microscopy? 4) Define: simple stain‚ differential stain and special stain. 5) What are the steps in a gram stain? 6) How can staining aid in the diagnosis of TB? 7) In general‚ can viruses be imaged with a light microscope? 8) Are all bacteria microscopic (beyond the ability to see with the naked eye)
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Lab Practical One Report Unknown Bacteria Staining Gram Acid Fast Spore The first practical for microbiology was staining of an unknown bacteria. Professor Goode provided an unlabeled bacteria in which each individual were to conduct gram‚ acid fast‚ and spore staining tests from memory. When the gram staining test was conducted a positive results was obtained. When views on oil bacteria appeared purple and rod-like. I noted this result and looked at the lab key to see what the results narrowed
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