there are distinct differences between the cell walls of gram-positive and gram-negative bacteria. In the case of gram-positive bacteria‚ the cell wall is comprised of 60-90% peptidoglycan and is very thick. There are numerous layers of teichoic acid bound with peptidoglycan thereby creating very thick cell membranes which causes the cell wall to take up large quantities of basic dye and appears purple. (Hands-on-Labs. (2012)). Conversely‚ gram-negative bacteria cell walls are much thinner with an
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Question 1 | | 1 / 1 point | Microbes are not responsible for ________. | | synthesis of acetone and alcohol | | | breaking down wastes | | | synthesis of vitamins | | | antibody production | | | oxygen generation via photosynthesis | View Feedback | | Correct! | Question 2 | | 1 / 1 point | The cell theory states that ________ are made up of cells. | | protozoa | | | archaea | | | plants | | | all living things | | | animals | Question
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Gram Negative Unknown Lab Report Abstract The Unknown Gram Negative bacterium inoculated in a Tryptic Soy broth medium was randomly selected from a group of other unknowns. In order to identify this unknown the seven different types of biochemical tests will be conducted on this unknown bacterium to identify it out of 6 possible bacteria; Escherichia coli(E. coli)‚ Enterobacter aerogenes (E.aerogenes)‚ Klebsiella pneumoniae (K.pneumoniae)‚ Proteus mirabilis(P.miranilis)‚ Pseudomonas aeruginosa(P
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bacteria? Gram Stain This separation is based on differences in what physical trait? The structure of the cell walls. What is the iodine used for in this staining technique? Crystal violet is first used to stain the sample and iodine is used to affix it. Why is it important to heat fix the bacteria prior to the staining procedure? This procedure is done to kill any bacteria that may be on the slide already and this will insure a clean slide. If you had a bacteria sample that you knew to be gram positive
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ABSTRACT In todays world of ever evolving genetic mutations the incidence of drug resistant forms of microorganisms are on the rise. The importance of identifying these pathogens and their related epidemiology has become increasingly more important. The purpose of this study was to identify an unknown bacterium in a controlled laboratory environment over a 5 week period. Utilizing a variety of differential testing and staining methods learned in the microbiology course‚ students were to determine
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Experimentation-outlines particular events predicted by hypothesis‚ then sets up experiments to test for the events Results-support or refute hypothesis (you never prove anything)‚ some part of hypothesis may be discarded or modified to fit results Conclusion or theory-repeated investigation of results History of Microbiology Spores and Sterilization Some microbes in dust and air were resistant to high heat—later identified as spores The term “sterile” was introduced which meant completely eliminating
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I was able to come to the conclusion that my unknown is Klebsiella pneumoniae through the use of Bergey’s Manual. After performing a gram stain of my unknown I was able to determine that my unknown was a gram-negative rod. While testing the oxygen requirements my test tube had growth throughout the tube‚ indicating my unknown was a facultative anaerobe‚ which can grow in the presence or absence of oxygen. The most important test was whether my unknown was motile or nonmotile. My test tube only showed
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has been used to teach the students how to stain and identify bacteria. There are several types of staining through which the bacteria can be identified based on the color and shape. The staining methods used in the lab are Gram Staining‚ Capsule Staining‚ Endospore Staining‚ and Acid Fast staining. One of the most significant method of staining is the Gram Staining‚ as it is highly dependent (McCarthy‚ 25). In the specific experiment that was done‚ Gram Staining was used and the bacteria that was
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between Gram Positive and Gram Negative Shapes of bacteria and examples What shapes have you learnt? GRAM STAIN: The process! You should work on your lab notes. LECTURE 2 GRAM STAIN Developed by a Danish called Christian Gram in 1884 Stain a heat-fixed smear with a dye like crystal violet and fix with Iodine Then wash with ethanol or acetone Finally counterstain with a dye of different colour such as safranin (what is its colour? You have used it before!) 2 5 Gram positive Gram negative
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Pure Culture Techniques In this first lab‚ you will be learning some very fundamental and important techniques. As is the case with most things‚ shorts cuts usually get you in trouble. This is especially true in Microbiology. The techniques you will be learning tonight‚ if mastered correctly‚ will make your life and learning experience in Microbiology much easier‚ if you don’t pay attention and practice these techniques incorrectly‚ well then……? Today you will be learning the following techniques:
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