the VirtualUnknown software and investigate how the components of different media can control bacterial growth. Deliverables: Each week‚ you will prepare a lab report detailing your experiments for the week. Include all data‚ observations‚ and conclusions in the single report. You should be sure to address the questions in the lab steps in the appropriate sections of the lab report. Your lab report will have the following format: Title Introduction: The first section of your lab report will be the
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If there is any update‚ it will be posted no later than this Saturday (2/8/14) Identify the following individuals’ major contributions to science Ehrlich – magic bullet Hooke –cell theory and microscope Jenner - cowpox Lister- disinfectant (phenol) Koch- kochs postulate Lancefield- immunology to classify via serotypes Linnaeus- nomenclature Pasteur- pasteurization‚ vaccine‚ microbes in nonliving matter‚ aseptic techniques‚ fermentation. van Leeuwenhoek- described live organisms with Hooke’s microscope
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spp. and Moraxella catarrhalis‚ ii. differentiate the Neisseria spp. and Moraxella catarrhalis associated with humans‚ iii. describe the isolation media used for Neisseria gonorrhoeae and give reasons why they are used‚ iv. recognize gram-negative diplococcic in urethral and cervical smears‚ and‚ v. identify: Neisseria meningitides‚ Neisseria gonorrhoeae‚ Neisseria lactamica‚ and Moraxella catarhalis. Method: Lab manual page(s) 27-8 Results: Part A) A‚ B‚ C‚ D unknown
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Introduction The working hypothesis being tested is if any of the three products; Scope Mouthwash (Scope)‚ LISTERINE® Antiseptic Mouthwash (Listerine)‚ Bactine Original First Aid Liquid (Bactine)‚ and Povidone-Iodine Solution‚ 10% Topical Microbicide Antiseptic (Iodine) are an appropriate antiseptic to can kill a sample bacterial contaminant found in the local environment‚ and if so‚ which is the most effective? The purpose of this experiment is to determine the antibacterial effectiveness of Scope
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culture was performed on a Tryptic Soy Agar Plate for isolation and purity and then incubated at 37 degrees Celsius for 24 hours. A gram stain was performed to determine whether the bacterium was a gram negative or a gram positive. After performing the gram stain‚ I concluded that by the appearance of purple spherical clusters resembling grapes that is was a gram positive cocci. A Catalase test was then performed using hydrogen peroxide. A positive catalase test was observed when bubbles started
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Gongora 12th December‚ 2014 MCB 3020L- Section 018 Unknown #16 Abstract This report contains the background information on gram positive and gram negative bacteria‚ which will aid in understanding the use of specific laboratory experiments to distinguish between the two types of bacteria. Included are the materials and methods used to identify the gram positive and gram negative bacteria and methods which also differentiate between microbes of each group. The implications of the methods of identification
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differential stain (gram stain) to determine the unknown microorganism cell shape and arrangement. The gram staining is done by first putting a sample of the unknown bacteria on a slide and it was heat fixed taking care not to over or under fixed the slide. The slide was flooded with crystal violet and let stand for 30 seconds and then rinsed with water. Secondly‚ the slide was treated with a decolorizing agent (ethanol) and after a minute it was washed with water and countered stain with safranin
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TYPES OF AGAR Mac Conkey‘s Agar plate MacConkey (also McConkey) agar is a culture medium designed to grow Gram-negative bacteria and stain them for lactose fermentation. It contains bile salts (to inhibit most Grampositive bacteria‚ except Enterococcus and some species of Staphylococcus)‚ crystal violet dye (which also inhibits certain Gram-positive bacteria)‚ neutral red dye (which stains microbes fermenting lactose)‚ lactose and peptone. QUALITY CONTROL Results after 24 hrs at 35º C Organisms
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question and how it functions. Not all of the tests were performed on every culture‚ however‚ as some of the tests were used only for gram (+) or gram () bacteria‚ while others were even more specific and used only for cocci bacteria. The tests performed and what constitutes a positive and negative test are as follows. The oxidase test was performed only on gram (-) bacteria and was used to test for the presence of cytochrome oxidase. Living bacteria were placed on a paper towel and saturated
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cell wall are and . The scientific names of any two (2) microorganisms that are not classified as prokaryotes are and The two (2) genera of bacteria that are capable of producing spores are and b) c) d) An example of a Gram positive bacteria is example of a Gram negative bacteria. and is an e) Three (3) common differential staining methods used in microbiology are ‚ and "Ubiquitous" in microbiology means The minimum inhibitory concentration (MIC) means Of more than three thousand
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