colony characterization and were estimated by their morphological and biochemical characters. As being a monsoon the occurrence of variation of different species were high. The microorganisms isolated from the soil were of staphylococcus strain and were gram positive‚ aerobic‚ coccus shaped bacteria. The fungal species were also identified‚ of which Aspergillus and Penicillium were dominant‚ followed by mucur‚ as sub dominant .This project aims to find out the water and soil quality of River and as it
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1.0 Introduction Figure 1: Restored Book Restoration can be defines as to return or make as before‚ to reestablish something‚ to bring back the material as the original form. In the other word‚ restoration is the process of improving the condition of every kind of archival and library materials as far as practicable. Restorative Techniques is an innovative supplier of surface cleaning systems and products. Its technical expertise of materials and substrates is based both on professional knowledge
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William Howard Taft Born: 9/15/1857 Birthplace: Cincinnati‚ Ohio William Howard Taft was born in Cincinnati on Sept. 15‚ 1857. A Yale graduate‚ he entered Ohio Republican politics in the 1880s. In 1886 he married Helen Herron. From 1887 to 1890‚ he served on the Ohio Superior Court; 1890–92‚ as solicitor general of the United States; 1892–1900‚ on the federal circuit court. In 1900 McKinley appointed him president of the Philippine Commission and in 1901 governor general. Taft had great success
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testing in order to determine the identity of the unknown organism. The tests performed to identify the unknown organism included Gram Staining‚ Fluid thioglycollate/aero-tolerance test‚ Methyl Red-Vogues Proskauer‚ SIM test‚ Lysine Decarboxylase test‚ Lactose Fermentation test‚ Simmons Citrate test‚ and TSI test. The Gram staining technique showed that the organism was Gram negative and bacilli shaped. The fluid thioglycollate test showed that the organism was a facultative anaerobe‚ meaning ATP can
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characteristics. Afterwards‚ a Gram stain was performed from a sample of the agar plate and the slide was viewed under the microscope. Once‚ the microscopic visual was captured‚ a catalase test followed. Next‚ for further data‚ MSA results were recorded‚ along with antibiotic observations of demo plates. Lastly‚ with the information gathered‚ it was identified that the unknown sample was Streptococcus salivarius. Introduction: In this lab‚ the exploration of an unknown gram positive bacteria was conducted
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viewed unstained‚ endospores of living bacilli appear edged in black and are very bright and refractile. Endospores strongly resist application of simple stains or dyes and hence appear as nonstaining entities in Gram-stain preparations. However‚ once stained‚ endospores are quite resistant to decolorization. This is the basis of several spore stains such as the Schaeffer-Fulton staining method which also differentiates the spores from sporangia and vegetative cells. However this method is not suitable
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because they provide accurate data. 2. Through this letter I would like to present my opinion regarding Grams staining method and reliability of this method. According to me‚ Grams staining process is a simple technique that assists in recognition of etiological agent and therefore can be called as one of the most significant staining practices in microbiology. Though‚ Gram is concerned that the staining process developed by him is imperfect because not all bacteria can be stained by it
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aerogenes ABSTRACT The objective of this report was to identify an unknown microorganism through several differential media tests. Over the course of a couple weeks‚ ten tests were performed. First‚ a gram stain was performed‚ indicating the bacterium was gram negative. An aerotolerance test determined that the bacterium was a facultative anaerobe. Next‚ a negative result in the methyl red test indicated that no mixed acid fermentation occurred. The DNase test was performed and
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1. Gram Stain * Verify if bacteria are present or not. * Controls – positive (purple) – S.aureus negative ( red/pink) – E.coli 2. Endospore Stain Positive controller – B. magneterium Green spore- Positive Pink (vegetative ) – Negative 3. Acid fast Positive control – M. smeagmatis Blue – negative Pink /red- positive 4. Motility Positive control - P.vulgaris A. Non motile is negative test B. Motile is a positive test 5. Carbohydrates fermentation (test for gram -) ( glucose
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When gram stained gram-negative bacteria stains pink or red; it is colorless and requires safranin as a counterstain. The cell wall of the bacterial cell is a complex‚ semirigid structure responsible for the shape of the cell (Tortora‚ Funke & Case‚ 2013). Gram positive bacteria occupy a multilayered thick peptidoglycan‚ and teichoic acid. Teichoic acids assist with distinguishes gram positive bacteria from other bacteria. The Gram positive bacteria are easier to kill. The gram reaction of
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