Introduction The acid-fast stain was developed in 1882 by Paul Ehrlich. Ehrlich was working with Mycobacterium tuberculosis‚ the bacilli responsible for tuberculosis‚ and found a technique that renders M. tuberculosis distinguishable from nearly all other bacteria. Acid-fast staining is‚ therefore‚ known as a differential stain. Mycobacterium and some Nocardia species are considered acid fast because‚ during the acid-fast procedure‚ they are able to retain the primary dye even when decolorized
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bacteria are not gram positive‚ but this lab will examine only this kind because of their influence in human health. Staphylococcus Aureus is a gram-positive coccus bacterium found in humans. It is frequently found on the mucus membrane in the nasal cavity and on the skin. It also infects many people in the hospital‚ also called a nosocomial infection. S. aureus has a large association with clinically significant infections and
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Unknown Lab Report #1 Unknown #1 April 25‚ 2012 Microbiology Spring 2012 MCB2010C Unknown #1 Introduction Identity of a microorganism has proven to be very significant. Doing so can help identify diseases and created treatment and cures for such diseases. As a result‚ various laboratory tests were performed to an unknown microbe (Unknown #1) found in the water of a nearby pond. By identify the microbe‚ the safety of the water will be known to those around it. Materials and Methods
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1. Gram Stain * Verify if bacteria are present or not. * Controls – positive (purple) – S.aureus negative ( red/pink) – E.coli 2. Endospore Stain Positive controller – B. magneterium Green spore- Positive Pink (vegetative ) – Negative 3. Acid fast Positive control – M. smeagmatis Blue – negative Pink /red- positive 4. Motility Positive control - P.vulgaris A. Non motile is negative test B. Motile is a positive test 5. Carbohydrates fermentation (test for gram -) ( glucose
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Title: Zinc and Copper II Sulfate Lab Purpose: To determine which mole ratio of Zinc and Copper II Sulfate produces the greatest temperature change in degrees celsius. Background: This experiment will be looking for color change‚ temperature change‚ and precipitation change. Some background knowledge I know is how to balance equations. I also know side effect of a chemical change‚ in this experiment there was a color and temperature change. Other background knowledge is using and applying the
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The purpose of this exercise was to use our skills we have learned in the lab to identify two unknown organisms. PROCEDURE I was given unknown #76. I performed the streak plating method with my unknown organisms. To perform this exercise I needed a TSA plate and labeled the bottom of it with my name‚ group number and organism. I also divided the bottom into three sections. After that‚ I sterilized my inoculating loop using the bunsen burner flame. After letting the loop cool‚ I reached into my
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Purpose: To find out the densities and to find out the name of the unknown metals. (Based on the extensive and intensive properties) Check up the words mass‚ volume‚ density‚ extensive properties‚ and intensive properties. Where do the units for mass and volume) come from and what do they mean? What is the density of distilled water? What is Archimedes principle? Does temperature affect the density of a solid? Liquid? Gas? Materials: Safety glasses‚ 10‚ 25 or 50 mL graduated cylinders
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Pseudomonas and identifying it using phenotypic properties and DNA sequencing as an existent or completely new and undiscovered species of Pseudomonas. Overview: Genus Pseudomonas is a chemoheterotrophic bacteria found in soil and water. They are Gram negative‚ motile‚ paired rods that are also oxidase-positive. Pseudomonas species are well known for their ability to metabolize many different organic nutrients‚ allowing them to be viable in multiple‚ diverse conditions. The Pseudomonas organisms
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will broaden range &may increase melting point Lab Questions 1. Define the following A. Melting point B. Intermolecular forces C. Hydrogen bonding 2. Arrange the following IMF according to there strength Dipole-Dipole interaction forces‚ Hydrogen bonding‚ Van der Waals forces 3. How does the atmospheric pressure affect the melting point? 4. What is the purpose of determining the melting point in this lab? 5. Why is this method not used for finding the melting
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In a positive test for starch the colour of the substance would change to black c) In a positive test for protein the colour of the substance would be pink or purple/violet d) In a positive test for lipids the substance would leave a translucent stain on the brown paper bag 2. The purpose of testing the four test tubes was to ensure that the colour change that occurred in the substance was due to a chemical change and not the substance being added to it. 3. The student saw a colour change happen
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