Microbiology Lab Quiz #2 Study Guide Exercise 9 -- The Gram Stain Compare and contrast simple and differential staining techniques. Simple Staining Simple staining is useful in determining the basic morphology of an organism. Simple staining involves only one reagent i.e. crystal violet‚ basic fuchsin‚ or methylene blue. Simple dyes used to stain bacteria have a positive charge cationic (+)‚ therefore‚ they are attracted to the bacteria that are usually negatively charged anionic (-) Depends
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CJ Tavner 2/04/2013 Chemistry Lab: Equilibrium and LeChatelier’s Princinple Objective: Put stresses on the systems; observe how the equilibrium’s systems react to a stress. Materials and Procedures: A. Materials 1. NaCl(s) 2. KSCN‚ 0.002M 3. Bromythymol blue indicator solution 4. AgNO3‚ 0.1 5. CoCL2 x 6H2O(s) 6. HCl‚ 12M 7. HCL‚ 0.1M 8. NaOH‚ 0.1M 9. Fe(NO3)3‚ 0.2M 10. C2H5OH(l) 11. Na2HPO4(s) 12.
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wikipedia.org/wiki/Salicylate http://en.wikipedia.org/wiki/Aspirin) Synthesis (procedure)- for the procedure I prepared a boiling bath using a hot plate‚ and filled a 400ml beaker with tap water. Then I got a 125ml Erlenmeyer flask and put in 2.00 grams of Salicylic acid using a scale. For the third step I added 5ml of Acetic Anhydride using a graduated cylinder. Then I added 5 drops of concentrated Sulfuric acid to the Acetic Anhydride and Salicylic acid in the Erlenmeyer flask using a dropper. I
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Biology 160 Lab Manual Tacoma Community College Last update 06/03/2013 This page left intentionally blank Table of Contents Table of Contents i Laboratory 1: Principles of the Scientific Method 4 In-Lab Report Sheet 12 This page has been left intentionally blank 27 Laboratory 2: Scientific Methodology & Enzyme Activity 28 In-Lab Report Sheet 28 Objectives 33 Introduction 33 Parts of the Swift M5 Microscope 33
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| Gram Negative Unknown | Biology 3444-006 | | Lena Wallace | 11/7/2011 | | Abstract: The purpose of this lab was to identify an unknown bacteria culture using differential tests. The identification of the unknown culture was accomplished by identifying the bacteria based on its specific metabolic characteristics and morphology. It is suggested that culture 11 is a sample of Enterobacter aerogenes. Introduction: This experiment was centered on metabolic and biochemical testing
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Observing Bacteria and Blood Abstract The purpose of this exercise is to gain knowledge on basic skills to correctly operate a microscope by viewing blood and bacteria through different magnifications Hypothesis This exercise will help me gain knowledge and the correct way to use a microscope. This will also help me for future observations of microbes. Methodology Properly set up microscope and made sure it was functioning properly. All lenses were wipe with lens paper and nine different
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(dialysis tubing). The experiment will show how molecules in solution move from areas of higher concentration to areas of lower concentration in the attempt to reach homeostasis in different circumstances. Introduction: The main purpose of this lab was to observe diffusion and osmosis. This is demonstrated using dialysis tubing and a combination of monosaccharaides‚ disaccharides‚ water (H20)‚ and sodium chlorine
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Preparation of an Ester Acetylsalicylic Acid (Aspirin) OBJECTIVE: To become familiar with the techniques and principle of esterification. DISCUSSION: Aspirin is a drug widely used as an antipyretic agent (to reduce fever)‚ as an analgesic agent (to reduce pain)‚ and/or as an anti-inflammatory agent (to reduce redness‚ heat or swelling in tissues). Chemically‚ aspirin is an ester. Esters are the products of reaction of acids with alcohols‚ as shown in the following equation using
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PRE LAB REPORT Reynaldo Riboul TLC and Column Chromatography October 6‚ 2013 Table of Chemicals: Chemical Hazards Mol. Wt. Density Grams Moles Acetone Flammable‚ Irritant 58.08 g mol−1 0.791 g cm−3 2.0 g 0.0344 Hexane Flammable‚ Irritant‚ Dangerous to Environment 86.18 g mol−1 .6548 g mL−1 9.0 g 0.1044 Fluorene Very toxic to aquatic life with long lasting effects 166.223 g/mol 1.202 g/mL 0.3 g 0.00180 Fluorenone Irritant 180.20 g mol−1 1.13 g/cm3 0.3 g 0.00166
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unknown bacteria lab assignment was to select an unknown bacteria culture and‚ through a series of metabolic tests‚ identify which bacteria genus resided in the pure culture received. A nutrient broth inoculated with bacterial culture (numbered 45‚ henceforth referenced as U45) was selected and a streak plate was made to isolate a pure culture for use throughout the assignment. From the streak plate‚ several slides were made to determine the morphology of unknown 45. A Gram stain‚ used to indicate
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