Describe the major differences between gram positive and gram negative bacteria cell walls. The gram negative bacteria cell wall is a thin peptidoglycan layer and an outer cell membrane with a lipopolysaccharide layer. The gram positive bacteria cell wall is a single thick peptidoglycan layer. This wall forms in a mesh like formation of three layers of alternating material. 2. From the procedure that you have carried out‚do you feel that the Gram positive stain is a simple procedure? No‚ because
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The Gram staining method is named after the Danish bacteriologist who originally developed it in 1882‚ Hans Christian Gram. The Gram staining process is one of the most important staining techniques in microbiology. It is almost always the first test performed for the identification of bacteria. The primary stain of the Gram’s method is either crystal violet or methylene blue‚ each almost equally effective. The microorganisms that retain the crystal violet and iodine mixture appear purplish brown
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Conclusions: We learned that some nasty stains can come out with some unorthodox products. We used on our stain Isopropyl alcohol (rubbing alcohol)‚ bleach‚ and shout. The product that did the best at pulling out stains was the Isopropyl alcohol. Isopropyl alcohol is a clear‚ colorless‚ flammable‚ mobile liquid‚ (CH3)2CHOH‚ used in antifreeze compounds‚ in lotions and cosmetics‚ and as a solvent for gums‚ shellac‚ and essential oils. It did great job at pulling stains right out of the cloth. This products
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able to attain at least 75% proficiency and should be able to: A. describe staining; B. enumerate the common stains; C. differentiate between an acidic dye and a basic dye; D. compare simple‚ differential and special stains; and E. list the steps in preparing a Gram stain and describe the appearance of a gram-positive and gram- negative cells after each step.\ II. SUBJECT MATTER: Biological Techniques Topic: Staining A. Materials:
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MICROBIOLOGY 3444-007 Gram Negative Unknown Unknown Bacteria #14 LeNaiya Kydd 4/2/2014 Abstract In order to be able to identify the unknown organism that was given to us‚ we had to conduct a number of different tests. These biological tests are used because they help us be able to identify the properties of the unknown we have and be able to compare our observed results with actual results of all the potential organisms. When all of the data of the test are put together it is easy to
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GRAM STAIN THEORY: The most widely used staining procedure in microbiology is the Gram stain‚ discovered by the Danish scientist and physician Hans Christian Joachim Gram in 1884. Gram staining is a differential staining technique that differentiates bacteria into two groups: gram-positives and gram-negatives. The procedure is based on the ability of microorganisms to retain color of the stains used during the gram
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Gram Staining of Bacteria Since bacteria is really small microbiologist use stains to help them see bacteria more clearly under the microscope. Many of the stains they use color the bacteria cells directly and are called direct stains. Bacteria cells have a slightly negative charge while direct stains have a slightly positive charge that helps the stain bin to the bacteria. The strength of the binding from the stains depends on the make-up of the cell wall itself. Gram staining is the most helpful
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Permanent Stain I stood peeking through the small living room doorway and watched my mother beat my sister. My mother had a full grip of her hair in one hand‚ and a thick‚ coiled wire in the other. She took a blow to her delicate skin‚ releasing all of her held in anger. I see my sister curled up on our dirty‚ kitchen floor‚ trapped between our wooden door and filthy fridge. I hear her cry as if she cried her lungs out for a stop to the blows she’s forced to take‚ and for a stop to the thought
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Medical Mycology: Yeast and Pneumocystis| Reading Assignment:|Mahon‚ Chapter 10‚ pgs 215-219‚ Chapter 27‚ pgs 626-629‚ 634-636‚ Appendix B Lecture Notes: Medical Mycology| |U of W Tutorial on Mycology (organisms listed in objectives)‚ www.medtraining.org[->0]| _____________________________________________________________________ 1. Discuss the difference between yeasts and molds. Fungi seen in the clinical laboratory can be generally separated into two groups based on the appearance of the
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1: Victoria ’s Ugly Stain Introduction: The purpose of this assignment is to give you an opportunity to apply some of the critical reading strategies you have evaluated. Resources: Go to Writing on the Run! to review the “Writing Process.” You may also want to review your English 11 Introduction Assignment for a tutorial on how to write a paragraph. Instructions: Complete the assignment by following the steps outlined below. STEP A: Read the article “Victoria’s Ugly Stain”; then answer questions
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