culture was performed on a Tryptic Soy Agar Plate for isolation and purity and then incubated at 37 degrees Celsius for 24 hours. A gram stain was performed to determine whether the bacterium was a gram negative or a gram positive. After performing the gram stain‚ I concluded that by the appearance of purple spherical clusters resembling grapes that is was a gram positive cocci. A Catalase test was then performed using hydrogen peroxide. A positive catalase test was observed when bubbles started
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LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
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PEES 4200W- Physiology of Exercise Lab #2: Metabolism and Energy Expenditure 1. The Respiratory exchange ratio is the ratio of the volume of carbon dioxide produced and oxygen consumed. The ratio indicates the energy that the subject is expending for indirect calorimetry‚ how efficient the subject ’s body is at utilizing the oxygen inhaled‚ as well as the main substrate being used for energy during varying intensities of exercise. During rest the volume of carbon dioxide was 0.73L/min‚ the volume
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An Essay On Poop Stains Issues surrounding poop stains can never be over analyzed. I find my self constantly drawn back to the subject of poop stains. While it is becoming a hot topic for debate‚ it is important to remember that ‘what goes up must come down.’ Since it was first compared to antidisestablishmentarianism much has been said concerning poop stains by so called ’babies’‚ whom I can say no more about due to legal restrictions. With the primary aim of demonstrating my considerable intellect
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Pseudomonas and identifying it using phenotypic properties and DNA sequencing as an existent or completely new and undiscovered species of Pseudomonas. Overview: Genus Pseudomonas is a chemoheterotrophic bacteria found in soil and water. They are Gram negative‚ motile‚ paired rods that are also oxidase-positive. Pseudomonas species are well known for their ability to metabolize many different organic nutrients‚ allowing them to be viable in multiple‚ diverse conditions. The Pseudomonas organisms
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chemicals before use. Abide by specific warnings and directions. 3. Collect all materials needed for a procedure before proceeding. 4. Perform reactions under the hood when directed. Chemicals may be weighed and prepared at balance or lab tables‚ but tests should be carried out under the hood. 5.Acids and caustic chemicals are stored in the hood. Please do not take these chemicals from the hood. Procedure: PART 1: Metathetical reactions Precipitation reactions A1. Add a
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Organic Chemistry II Lab 9 Fermentation of a Carbohydrate: Ethanol from Sucrose * Introduction Ethanol is one of the oldest alcohols and also the least toxic one. Industrially‚ ethanol is made most economically by hydration of ethylene. However‚ ethanol that is intended for human consumption must‚ by law‚ be prepared by fermentation. By either method‚ ethanol‚ of course‚ has the same formula‚ structure‚ and properties. The fermentation takes place with the assistance of enzymes from yeast
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concentrations of solutes in and out of the cell. Filtration is what is used to remove solid particles and they can be removed by passing through a liquid and gas. The information above is very important because it is exactly what everything in this lab will be about. It explains in detail what every single little definition and important
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of the Pineapple peeling as stain remover. We observe that people who can’t afford commercialized stain remover use vinegar as alternative. And mostly of the vinegar are made up in coconut‚ apple and etc. We want to find out if pineapple peeling extract is effective as stain remover. That’s why we come up with this project/ experiment. Statement of the Problem and Objectives 1. To prove the effectiveness of the Pineapple Peeling as a stain remover 2. To know how long
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Crystallization: Lab Report Theory/Introduction The purpose of this lab is to understand the concept of purification through crystallization. Crystallization is the process of purifying a substance‚ with the most adequate solvent. The process of crystallization came to be based on the principle of solubility. First‚ we must choose a solvent. The solvent is crucial to crystallization because it’s what will allow impurities to dissolve. Therefore‚ when selecting a solvent it is important that it
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