Amanda Lab Report 1 Microbiology Laboratory Title – Differentiating Organisms using the Gram Stain Introduction The experiment conducted was based upon the known attributes of two different groups of bacteria‚ those that are gram positive‚ and those that are gram negative. Using a specific staining procedure‚ it is possible to differentiate the two types under a microscope The gram stain method of differentiation is possible because of differences in the cell membrane between the two categories
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INTRODUCTION The Gram Negative lab had two parts; the aim of the first part is to determine the concentration of gram negative bacteria in a water sample collected from a creek near Providence Road‚ Strickling. Gram negative bacteria have a cytoplasmic membrane‚ a thin peptidoglycan layer‚ and an additional outer membrane composed of phospholipids and lipopolysaccharides. Because gram negative bacteria have a relatively thin cell wall when compared to gram positive organisms‚ they are consequently
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Gram Stain: Gram is an empirical method to distinguish the species of bacteria into two groups Gram-positive and Gram-negative based on the physicochemical properties of the cells. First‚ a smear was prepared by use a sterile transfer loop that been flamed to removes some bacteria from slant agar and placed on the slide; mixed with one drop of water and let air dried. After dried‚ heat fixation the slide by passed the slide over a flame quickly 2-3 times to stick bacteria to the slide. Next‚ the
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The purpose of this lab is to isolate a bacterial population from the normal throat flora. A streak plate method will be used to obtain a pure culture of a Gram positive coccus genus of bacteria. Several biochemical tests will be performed to aid in the identification of this unknown bacterium. Biochemical tests are a series of tests used to identify certain bacterium The various tests that are used in this lab are the catalase test‚ oxidase test‚ blood hemolytic test‚ MSA‚ blood agar‚ and PEA/ab
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Gram Staining Introduction Prokaryotes are a large group of organisms with no membrane bound organelles. They consist of two domains: Archaea and Bacteria. These organisms are only found in extreme environments such as volcanoes. Prokaryotes are still being researched and are a very diverse group. In this lab we focused on trying to identify if the bacteria found had a lot of peptidoglycan by gram staining. Testing this could be done by using a Petri dish full of agar and testing different bacteria
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MICROBIOLOGY 3444-007 Gram Negative Unknown Unknown Bacteria #14 LeNaiya Kydd 4/2/2014 Abstract In order to be able to identify the unknown organism that was given to us‚ we had to conduct a number of different tests. These biological tests are used because they help us be able to identify the properties of the unknown we have and be able to compare our observed results with actual results of all the potential organisms. When all of the data of the test are put together it is easy to
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Bio Lab Report Title: Gram-Negative and Gram-Positive contamination in yogurt. Introduction: There is bacteria that is beneficial and harmful in the human. Yogurt was choosed to be the best example that contains this bacteria. What happens to be the difference between these two types of bacteria‚ and how are they harmful? The bacteria is considered “Gram-Negative‚” and the substance is considered contaminated when there is more negative bacteria than positive bacteria. This will prove or disapprove
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Exercise 3.9 Endospore Stain (Differential Stain) Eula Lewis Bio 2921 February 27‚ 2013 Zere Ezaz‚ Ph. D. Objective: 1. To learn to endospore stain 1. To distinguish endospores‚ free spore‚ and vegetative cells Theory: A dormant form of bacterium that allows it to survive poor environmental conditions are called endospores. Their tough outer covering is made of keratin and is also the reason they are resistant to heat and chemicals. The strength of keratin also makes it resist
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Micro Biology Lab Gram Staining Lab Report Purpose The purpose of this lab was to experiment with a staining technique called Gram Staining. Gram Stains is used to identify and classify bacteria‚ it is a differential stain that allows you to classify bacteria as either gram-positive or gram-negative. The foloowing is a breif summerary of the gram staining procedure. Apply the primary stain Apply Mordant Apply decolorizing agent Apply the secondary stain Bacteria that
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GRAM STAINING EXPERIMENT CONDUCTED ON 9/29/2013 Introduction: The Gram stain is a useful stain for identifying and classifying bacteria. The Gram stain is a differential stain that allows you to classify bacteria as either gram positive or gram negative. This gram stain technique was discovered by Hans Christina Gram in 1884. The gram stain procedure separates all bacteria into one of two groups - into gram-negative bacteria which do not stain purple and into gram-positive
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