1 1. Explain the principle of positive staining. (3 marks) Positive staining is done by staining the cell walls of Gram-positive bacteria with crystal violet. The cell wall of Gram-positive bacteria is made up of a thick layer of peptidoglycan which undergoes dehydration during decolourisation causing the pores to shrink. This shrinkage of pores traps CV-I complex and stain the cell purple. 2. Using a flow chart‚ outline the process of gram staining. (5 marks) Flood the slide with crystal
Free Bacteria Staining Gram staining
stain. This study aims to determine the potential of methanolic extract of eggplant (Solanum melogena) peels as a dye pigment in differential stain. Eggplant contains anthocyanin in the peels. Eggplants were peeled and then weighed. Fifty (50) grams of peels were homogenized with acidified
Premium Bacteria PH Acetic acid
contaminated dirt by running it through a series of identification techniques. Some of these techniques were simple staining‚ gram staining‚ and isolation techniques. The hypothesis of this experiment was that the bacterium is of the bacillus family due to the rod shape. Due to multiple complications with the PCR‚ a new hypothesis couldn’t be formulated. Methods: In this experiment‚ a 1 gram sample of contaminated dirt was taken and mixed with 100 mL of sterile water then shaken. After allowing the sediment
Premium Staining
purpose of staining samples?A) To increase the contrast and visibility of the specimen B) To kill the specimen C) To see the specimen without the aid of a microscope D) To make the specimen appear larger in the microscope2.Which of the following are bacterial SHAPES? (Select all that are correct. Be sure to select the appropriate scientific name. You lose points for choosing incorrectly or for not choosing a correct choice.)A) Vibrio B) Strepto C) Spirillum D) Staphylo E) Cocci F) Bacillus G) Diplo3
Free Bacteria Staining Gram staining
different cultures were observed. In order to proceed identification‚ those cultures were isolated as separate organisms‚ using inoculating streak technique and incubated until the next class period. The expected result was supposed to show Gram positive and Gram negative bacterium growing on a separate media. Having checked two Nutrient plates‚ signs of visible growth of two different bacterium were present. Due to the fact‚ that one type of bacteria didn’t appear to be fully
Premium Microbiology Bacteria Staining
Exercise 8-B Differential Staining Gram Staining and Acid Fast Staining Introduction: Differential Staining‚ one which facilitates differentiation of various elements in a specimen is a general term that can refer to a number of specific processes. Using multiple stains can better differentiate between different microorganisms or cellular components of a single organism. Gram’s Stain is a widely used method of staining bacteria as an aid to their identification. It was originally devised by Hans
Premium Staining Bacteria Gram staining
particular order. Name this Procedure Identify the two types of bacteria present by shape and gram stain. In a gram stain what is the primary stain? The mordant? The counterstain? How does this differ from a simple stain? Identify the shape and gram stain of the bacteria present in this gram stain. Identify the shape and gram stain of the bacteria pictured. Think on this one! Name the gram stain and shape of the bacteria pictured. Name the genus of the organism pictured. Note the size
Premium Staining Gram staining Bacteria
bacteria and how they stain‚ whether it be gram positive or gram negative. Hypothesis: If I read the textbook and use the resource websites‚ then I will be able to better understand prokaryotes and describe the structural characteristics of viruses. By understanding prokaryotes‚ I can compare them to eukaryotes and learn more about the different types of prokaryote species. Experiment: Materials: Campbell Biology Textbook Lab 2 Prokaryote packet Website for gram stains: http://learn.chm.msu.edu/vibl/content/gramstain
Premium Bacteria Microbiology
Materials 1. Nutrient agar plates of a) Sarcina lutea and Serratia marcescens b) Bacillus subtilis and Escherichia coli c) Streptococcus fecalis and Pseudomonas fluoroscens 2. 24 hour pour plate preparation of a) Serratia marcescens b) Sarcina lutea c) Bacillus subtilis d) Escherichia coli 3. 24 hour agar slant of the following cultures incubated at 25ºC a) Bacillus subtilis b) Escherichia coli c) Serratia marcescens d) Pseudomonas fluoroscens 4
Free Bacteria Staining
tuberculosis. It is a small rod shaped bacillus which can survive in a dried or disinfected state for several weeks thanks to its thick lipid cell wall. Moreover‚ its thick lipid wall allows them to survive in the macrophages by impeding the fusion of lysosome with phagosome. This trouble-making characteristic of MTB made its identity obscured to be the diagnosed at the time when it was first discovered. This was because MTB was neither Gram negative or positive when it was Gram stained. Finally‚ the scientists
Premium Tuberculosis Bacteria Infectious disease