Microbiology Lab Quiz #2 Study Guide Exercise 9 -- The Gram Stain Compare and contrast simple and differential staining techniques. Simple Staining Simple staining is useful in determining the basic morphology of an organism. Simple staining involves only one reagent i.e. crystal violet‚ basic fuchsin‚ or methylene blue. Simple dyes used to stain bacteria have a positive charge cationic (+)‚ therefore‚ they are attracted to the bacteria that are usually negatively charged anionic (-) Depends
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incubated at 37C for 24- 48 hours‚ and two separate morphologies were then observed and recorded‚ unknown A and unknown B‚ a Gram stain was then performed for each unknown. After determining the Gram reaction‚ alternate staining procedures were performed as needed. Unknown A required further endospore staining‚ whereas unknown B required no further staining procedures. Once all staining methods were complete‚ specific biochemical tests were performed. The biochemical tests were chosen from the unknown
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_Control_______ variable. Through the semester‚ you have used two bacteria that produce colorful colonies: one produce yellow colonies‚ another one pink. What are the names of these two bacteria? Are they Gram+ or Gram-? Micrococcus Luteus‚ yellow colonies‚ gram + Serratia Marcescens‚ pink colonies‚ gram - There was one bacterial
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Bibliography: © The McGraw−Hill Companies‚ 2002 E X E RC I S E © The McGraw−Hill Companies‚ 2002 Gram Positive 38. General Unknown © The McGraw−Hill Companies‚ 2002
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ASEPTIC TECHNIQUES AND CELL COUNTING INTRODUCTION:- Microbes are single celled organisms and are so small that we cannot see with our naked eyes‚ microbes can only seen under microscopes .Microbes are the one of the oldest living form on earth‚ fossils of microbes were found which are said to be 3.5 billion years old . Microbe world: what is a microbe [online].[Accessed 9 April 2013].Available from: world wide web:‚microbes are found everywhere in rocks‚air‚water some of the microbes are even
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Microbiology Lecture Exam #1 Outline Chapter 1: Microbiology Introduction I. Microbiology Definition II. History of Microbiology (Christian Gram‚ Robert Koch‚ Antony van Leeuwenhoek‚ Robert Hook‚ Edward Jenner‚ Louis Pasteur‚ Elie Metchnikoff‚ Dmitri Iwanowski‚ Paul Ehrlich‚ Alexander Fleming) II. Scope of Microbiology A. Infectious Agents 1. Prions 2. Viroids 3. Viruses B. Prokaryotes
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Review all chapters in your exercise manual beginning with the introduction INTRODUCTION: (Covered 9.4.14 II Week 1) Biosafety levels1: basic level of containment. Hand washing or wearing gloves 2: Appropriate for working with human body fluids. Autoclave‚ sharps containers‚ lab coats 3: appropriate for working with pathogens that can be transmitted via respiratory route. Self-closing‚ double doors and sealed windows 4: Highest level. Aerosol pathogens; pathogens with no vaccine/treatment. Separate
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similar in their chemical composition and chemical reactions. Prokaryotes have peptidoglycan in the cell wall. Prokaryotes are Bacteria that are unicellular. Most Bacteria multiply by binary fission. There are three kinds of bacteria shapes: coccus‚ bacillus and spiral.
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A Comparative Study of Dalandan and Kamias as Decolorizer in Gram’s Staining Staphylococcus aureus and Escherichia coli An Undergraduate Research Presented to The College of Medical Technology Centro Escolar University In Partial Fulfillment Of the Requirements of the Degree Bachelor of Science in Medical Technology By: Aduana‚ Frances Mae D. Barnachea‚ Erika Joy V. Decleto‚ Pearl Yvette P. Lagmay‚ Ira Alexis C. Paris‚ Dublin Justin Patron‚ Chronielle Albert C. Radovan‚ Denzel James C
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state the meaning of each name. • Streptococcus is spherical in shape and commonly seen in strep throat • Lactobacillus is bacillus or rod shaped and found in probiotics • Spirillum Pulli is spirillum or spiral shaped‚ and evident in rat bite fever Exercise 3: Gram stain What is the function of the iodine solution in the Gram stain? If it were omitted‚ how would staining results be affected? Omitting the iodine in the gram’s stain would mean‚ the crystal violet that goes into the cell would
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