Effectiveness of Basella rubra (Alugbati) Fruit Extract as a Counterstain in Gram Staining Investigators: Astilla‚ Yasmin Marie Cala‚ Maria Victoria Bianca Ko‚ Mariell Lizardo‚ Francis Dominic Mariano‚ Maria Cristina Querol‚ Maria Zennah Quinto‚ Camille Rosales‚ Ana Mariella Valmonte‚ Kyla Nicole Villalon‚ Gian Angelo 4MT-2 Statement of the Problem Can Basella rubra fruit extract be used as a counterstain in Gram staining? Introduction & Background According to H. J. Croxx‚ when microscopists
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LAB REPORT NUMBER TWO DATE: 3/25/2010 inal attachment Lab Experiment number 11 PURPOSE: To learn the Gram stain technique‚ the reason for the stain‚ and how to identify the results of the organisms stained. MATERIALS: Bunsen burner‚ inoculating loop‚ staining tray‚ glass slides‚ bibulous paper‚ lens paper‚ oil‚ and microscope METHODS: Apply Crystal Violet (Primary stain) for 1 minute. Rinse with D-water Apply Iodine (Mordant) for 1 minute. Rinse with D-water. Apply Alcohol (Decolorize) for
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Pets as carriers of soil inhabitant microbes into our homes GIZELL VASQUEZ Biology department‚ Pathogenic microbiology‚ Crosby Jones‚ Angelo State University‚ San Angelo‚ TX 76909 There are approximately 78.2 million owned dogs in the United States (1)‚ and according to the United States government the current population of the nation is 308.7 million. The portion of dogs to humans is 4:1. These statistics are of importance because these pets physiologically observed their environment by smell
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Bacterial Smears Are Fixed before Staining to? Answer It is important to heat fix the bacterial smear before staining so as to‚ kill the bacteria‚ firmly adhere the smear on to the microscopic slide to prevent washing off during staining‚ and to allow the sample to readily take up the stain. Reference: www2.hendrix.edu What is the purpose of heat- fixing the smear? It helps the cells adhere to the slide so that they can be stained. The purpose of heat fixing is to kill the organisms without
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colored colony. Gram-staining was utilized as a first step to differentiate between gram-positive and gram-negative. After determining that it was a Gram negative single bacillus‚ biochemical tests performed and their purpose and results are shown in table and flow chart form. Table 1: biochemical Test Results Test | Purpose | Reagents | Observations | Results | Gram stain | To determine the Gram reaction of the bacterium | Crystal violet‚ Iodine‚ Alcohol‚ Sarasin | Pink rod | Gram-negative rods
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1- Synthesis of silver nanoparticles by Bacillus sp.: The microorganisms in the most contaminated toilet Sink were isolated by dilution technique. The dominant bacterial strains are identified as Bacillus sp.‚ on the basis of morphological and biochemical characteristics. Gram’s staining: Gram positive and rod shape. They were serially diluted and spread on nutrient agar plates. The starch has been hydrolyzed and a clear zone by the addition of iodine solution. Visual identification: When bacterial
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Bacteria Prokaryotic & Eukaryotic Cells 1-7.Bacteria cells reproduce ASEXUALLY BY BINARY FISSION. Asexually means the offspring comes from one parent Draw what Binary Fission of a bacteria looks like: 8. Label the parts of this prokaryotic cell. [pic] 9. Name several structures that are found in eukaryotic‚ but NOT prokaryotic cells. 10. What type of cells are the most numerous on Earth? 11. What are the most common type of prokaryotic cells? 12. How old are the earliest
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identified using a method called Gram staining‚ whereby a stain is used to differentiate different types of organisms. Bacteria with and without a cell wall are classified as Gram-positive or Gram-negative‚ respectively. Bacterial types vary by shape and size. Coccus Coccus refers to spherical shaped bacteria that may live as individual cells‚ or pair up to form chains or bunches. Staphylococcus and Streptococcus are the most common bacteria in this group and usually are Gram-positive. These bacteria perform
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Micrococcus luteus. There were ten bacteria species possibilities: Stahylococcus epidermidis‚ Bacillus subtilis‚ Escherichia coli‚ Serratia marcescens‚ Sarcina lutea‚ Pseudomonas fragi‚ Micrococcus luteus‚ Alcaligenes faecalis‚ Clostridium sporogenes‚ and Micrococcus roseus. There were several qualitative tests that could be conducted to determine the identity of the unknown species‚ for example‚ Gram staining‚ Fermentation‚ Catalase‚ Oxidase‚ Starch Hydrolysis‚ Litmus Milk‚ MOI medium‚ and the Gelatin
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bacteria and Archaea and eukarya are types of eukaryotes. * Archaea are more closely related to eukaryotes. * Prokaryotes have a membrane and nucleoid and most have a cell wall. * There are 3 main types of shapes of prokaryotes: * Bacillus – rod shapes * Spirilum – spiral shaped * Coccus – spherical * If they cells are a similar shape‚ that means they are adapted to live in a similar environment‚ and not necessarily closely related. * P are smaller and do not
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