results. This helps to slowly eliminate any bacteria that do not correspond with the results. I obtained tube number twelve to run various tests on. There are only two microorganisms‚ one is Gram positive and one is Gram negative‚ inside the test tube. The Gram positive possibilities are to be narrowed down from Bacillus cereus‚ Clostridium perfringens‚ Corynebacterium xerosis‚ Enterococcus faecalis‚ Streptococcus agalactaie‚ Staphylococcus aureus‚ and
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I were given unknown number 3 in the laboratory. After performing various tests over the course of a few weeks on our unknown‚ we came to the conclusion that our unknown organism is Klebsiella pneumoniae (K. pneumoniae). K. pneumoniae is a gram negative bacillus shaped microorganism. We observed that K. pneumoniae is a nonmotile organism. We performed multiple tests on our unknown culture‚ therefore we are very confident that it is correctly identified. We identified that K. pneumoniae is a facultative
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tube by running a series of experiments to detect which specific Gram negative organism we had. To detect your gram positive from the mixed culture was given as extra credit points also. A Gram stain was performed and isolation streak plate in order to isolate and observe the unknown organism. Before the series of test‚ a dichotomous key had to be written up in order to know what steps and tests to run to identify the unknown Gram negative organism. I had to run three experiments in all before I
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by Gram staining Gram positive: purple-blue‚ mostly peptidoglycan susceptible to penicillin Gram negative: pink-red‚ less peptidoglycan‚ has lipopolysaccharides‚ susceptible to EDTA Gram negative can be further differentiated into chlorophyllous (cyanobacteria) and achlorophyllous. Based on Gram staining Gram negative Escherichia coli Rhizobium Spirillum Pseudomonas Nostoc - cyanobacteria Oscillatoria - cyanobacteria Gram positive Staphylococcus aureus Streptococcus Bacillus Nostoc:
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prokaryotes. Bacillus (rod-shaped) Coccus (spherical/ovoid-shaped) Spirillum (long and helical-shaped) 7.) The bacterial cell wall is the single most important contributor to cell shape. 8.) Prokaryotic cell walls are constructed of many layers‚ which is formed from peptidoglycan polymers cross-linked by peptide side chains. 9.) Describe a Gram Stain. Staining technique that divides bacteria into gram-negative or gram-positive based on retention of a violet dye 10.) Distinguish between gram positive
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gyMicrobiology: is a study of microorganisms (microbes‚ germs) and their activities Microorganism: An organism or a virus too small to be seen without a microscope (smaller than 0.5mm) Misconceptions of Microorganisms * All microorganisms are pathogenic (disease producing) FALSE * The majority of bacteria are harmless and beneficial TRUE Aspects of microbiology * Basic biological science * About living cell and how they work * About microbial diversity and their evolution
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Screening and identification of rhizobacteria isolated from heavy metal contaminated soils INTRODUCTION As a result of human activities such as mining‚ agriculture and industrial activity‚ heavy metal pollution has become one of the most serious environmental problems today. Elevated levels of heavy metals not only decrease soil microbial activity and crop production‚ but also threaten human health through the food chain. Soil microorganisms can degrade organic contaminants‚ while metals need
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in the staining methods‚ the cells and their borders were very distinct while the one in the wet mounts were not as distinct. It was a bit challenging to determine the figures in the cheek smear‚ but the bacteria form in the yeast wet mount were easily recognized. The direct staining was the following technique used. The cells were very easy to assess‚ well demarcated and had a very distinctive color. Instead of the background and around the cell in the cheek smear‚ the indirect staining way seemed
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plates were moved to a 4℃ environment to be stored until they were Gram stained using the process outlined in Friedman’s lab handout (Friedman 2017). Liquid cultures were made (from the streak plates stored) every seven days on the day before Gram staining. Observations of the milk samples‚ including pH‚ odor‚ and color‚ were recorded daily. The liquid cultures and plates were Gram stained every seven days. Observations of the Gram stained samples made under the microscope were
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MBK Lab 01 – Lab Report Name: ____________________ Section: ___________________ EXPERIMENT 1 TITLE: Observing Bacteria and Blood OBJECTIVE: To gain functional knowledge of microscope operations through practical applications of a microscope in the observation of bacteria and blood. PROCEDURES: Using the microscope‚ an oil immersion lens and observing Bacteria Cultures in Yogurt . Preparing a Blood Slide and observing Blood: After reviewing the section of the manual
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