Week 1 Chapter 3 Staining Lab Quiz SCI/250 SCI250 Week 1 Chapter 3 Staining Lab Quiz (6.7 points each) Check or underline the correct answer Section: Multiple Choice 1. Which of the following stains is used frequently to identify Mycobacterium and other bacteria whose cell walls contain high amounts of lipids? Gram stain Schaeffer-Fulton stain Acid-fast stain Lipid stain Spore Stain 2. Which of the following stains is used to classify microorganisms based on their cell wall
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required for bone tissue engineering to eliminate bacterial infection in orthopedic implants which generally leads to implant failure. Table 4 showed antibacterial zone of inhibition of CMZH I-III and CZH against gram-positive bacteria Bacillus cereus and Lysinibacillus fusiformis and gram-negative bacteria Escherichia coli XL1B. It was observed that the antibacterial inhibition zone increased from CMZH I to CMZH III against all three bacterial strain where OMMT content has been increased from 5 to
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provide accurate data. 2. Through this letter I would like to present my opinion regarding Grams staining method and reliability of this method. According to me‚ Grams staining process is a simple technique that assists in recognition of etiological agent and therefore can be called as one of the most significant staining practices in microbiology. Though‚ Gram is concerned that the staining process developed by him is imperfect because not all bacteria can be stained by it‚ but in my opinion
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enzymes‚ and biochemical reactions. Qualitative observations were made on the tests in identification process. The data from experimentation provided sufficient results to suggest that the identity of unknown bacterium was the gram-positive spore forming bacteria‚ Bacillus Subtilis. Introduction. One of the main responsibilities of a medical lab is to determine the identity of pathogenic bacteria. It is important to determine the specific type of bacterium causing disease so the physician
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The steaks that were done on this plate seemed to show that E. coli did not have lipases because it did not have clear zones. Staphylococcus aureus seemed to have these clear zones meaning it can secrete lipases. The bacteria Bacillus subtilis did not grow well to see if there was a clearing. After doing some research‚ it was shown that B. subtilis is lipase positive so there should have also been some clear sones like the Staphylococcus aureus (Watson). The DNAse agar could not
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Anthrax is an infectious disease and it is caused by Bacillus anthracis. It is also called Splenic Fever‚ or Charbon. There are three types of Anthrax‚ there is cutaneous (anthrax of the skin‚) inhalation anthrax‚ and gastrointestinal anthrax. Anthrax is a large‚ rod shaped bacterium. It mostly affects animals but also can be transmitted to humans. Anthrax’s first incident was in 1500 B.C. in the early writings of Mesopotamia and the Book of Genesis. The Old Testament description of the 5th
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coli). Bacillus subtilis is a gram-positive rod-shaped bacterium‚ typically found in vegetation and soil. (Entrez Genome Project). E. coli is gram-negative bacterium commonly found in the gut of warm-blooded organisms. (National Health Service) HYPOTHESIS If we were to compare the antibacterial effects of various antibiotics (Penicillin
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Gram Characteristics of Bacteria Introduction Bacteria are interesting little critters in that they are not easily visualized without some sort of stain. In classical methodology‚ two different kinds of stains have been used to identify bacteria: acidic and basic dyes. Acidic dyes are so called because they consist of a salt that has a cation that transfers no color‚ but the anion portion is colored and does give off color. Basic dyes consist of an anion that does not give off any color and a cation
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become directly stained. In direct staining‚ the organisms must be fixed by a process such as heat. Fixing the slide prevents the organism form washing off the slide before visualization. This is accomplished by passing a smear of the bacteria through flame. The heat sets the proteins of the organism thus causing the bacteria to attach to the slide. The organism can become damaged from the setting process and the use of heat prior to staining. In indirect staining‚ the negatively charged colored portion
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Microbiology report Gram stain Objectives: The aim of this experiment was to be able to stain bacteria correctly and be able to see it under the light microscope. In 1883 Hans Christian Gram discovered an important staining method that is used extensively today. The stain is called the Gram Stain. This experiment was done in order to differentiate microbes into two basic groups: Gram positive microbes and Gram negative microbes. The purpose of this experiment was to learn the gram staining method and to
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